| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
Human Endogenous Metabolite
Calcium phytate targets divalent and trivalent metal ions (e.g., Ca2+, Fe3+, Zn2+, Pb2+) via its multiple phosphate groups. It functions as a potent chelator, forming stable complexes. It also modulates the bioavailability of dietary minerals such as calcium, iron, and zinc. |
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| ln Vitro |
In vitro, calcium phytate is a complexing agent used to remove traces of heavy metal ions. It also acts as a hypocalcemic agent (calcium-lowering). It exhibits antioxidant, anticancer, and anti-inflammatory properties. The compound can chelate metal ions, reducing oxidative stress and inhibiting lipid peroxidation. In vitro studies have also demonstrated its potential in preventing kidney stone formation and modulating blood glucose levels.
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| ln Vivo |
In vivo, calcium phytate (20 g/kg; feed administration; 8 days) has a mitigating effect in a mouse model of lead poisoning. It reduces blood and tissue lead levels due to its metal-chelating properties. It also has a hypocalciuric effect: phytin and magnesium citrate reduce urinary calcium excretion in rats fed high-calcium diets. A six-day treatment with phytin decreases urinary calcium output. It may also have beneficial effects on kidney stone formation, blood glucose modulation, and cancer prevention in animal models.
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| Enzyme Assay |
For non-cellular assays (metal chelation), a colorimetric assay for iron chelation is used. The compound is incubated with FeSO4 (100 uM) in 50 mM HEPES (pH 7.0) for 30 minutes at 25degC. The ferrous iron concentration is determined using Ferrozine (3 mM) which forms a magenta complex (absorbance at 562 nm). The percentage of chelation is calculated as (1 - [Fe2+ in sample]/[Fe2+ in control])×100. For antioxidant assays, DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging is measured at 517 nm.
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| Cell Assay |
For cell-based assays, human intestinal epithelial cells (Caco-2) or hepatocytes can be used. Cells are seeded in 24-well plates (1×10⁵ cells/well) in DMEM with 10% FBS. After 24 hours, cells are treated with calcium phytate (0-500 uM) for 24-48 hours. Cell viability is assessed by MTT assay. For oxidative stress studies, cells are pre-treated with calcium phytate for 24 hours, then exposed to H2O2 (100-200 uM) for 4 hours. ROS is measured by DCFH-DA (10 uM, 30 min). For mineral transport studies, Caco-2 monolayers grown on Transwell inserts are used, and mineral (⁶⁵Zn or ⁵⁹Fe) transport is measured after addition of calcium phytate.
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| Animal Protocol |
For in vivo animal experiments, a mouse model of lead poisoning is used. Male ICR mice (6-8 weeks old) are given lead acetate (0.5% in drinking water) for 8 days. Calcium phytate is mixed into the feed at 20 g/kg (approximately 400 mg/kg/day based on feed intake) for 8 days. At the end of the study, blood, liver, and kidneys are collected. Lead levels in tissues are measured by atomic absorption spectrophotometry. For calcium metabolism studies, rats (e.g., Sprague-Dawley) are fed high-calcium diets (1-2% Ca) with or without calcium phytate (1-2% of diet) for 6-14 days. Urine and feces are collected in metabolic cages. Calcium excretion is measured by atomic absorption. Blood calcium levels are measured by colorimetric assay.
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| ADME/Pharmacokinetics |
Phytin has a molecular formula of C₆H₆Ca₆O24P₆ and a molecular weight of approximately 888.42. It is a white to off-white solid powder, insoluble in water (solubility ~8.7 mg/mL). It contains six calcium ions per molecule, giving it high negative charge density and strong metal-chelating capacity. It is stable as a dry powder and should be stored at -20degC in a sealed, dry environment.
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| Toxicity/Toxicokinetics |
Calcium phytate has low acute toxicity. The calcium salt form is less soluble than other phytate salts, which limits systemic absorption. In animal studies, it is well-tolerated at doses up to 20 g/kg in feed. However, long-term high intake can reduce the bioavailability of dietary minerals (iron, zinc, calcium). It is generally recognized as safe (GRAS) for use as a food additive. Standard laboratory safety precautions should be followed.
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| References | |
| Additional Infomation |
It is a complexing agent used to remove trace heavy metal ions. It also has a calcium-lowering effect.
Calcium phytate (phytin) is a dietary component and research compound, not an approved drug. It has not undergone clinical trials for therapeutic use. It is used in research to study mineral metabolism (calcium, iron, zinc bioavailability), heavy metal detoxification (lead poisoning), and as a complexing agent. It has also been investigated for its antioxidant, anticancer, and anti-inflammatory properties. In the food industry, phytin is used as a preservative and a chelator. As an endogenous metabolite, it is classified as a natural product. |
| Molecular Formula |
C6H14CAMGO24P6
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|---|---|
| Molecular Weight |
720.399
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| Exact Mass |
887.543
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| CAS # |
3615-82-5
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| Related CAS # |
Phytic acid;83-86-3;Phytic acid dodecasodium hydrate;123408-98-0;Phytic acid hexasodium;34367-89-0
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| PubChem CID |
19234
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| Appearance |
White to off-white solid powder
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| Density |
2.42g/cm3
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| Boiling Point |
1190.7ºC at 760mmHg
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| Flash Point |
673.9ºC
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| LogP |
2.125
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
24
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
38
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| Complexity |
749
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C1(C(C(C(C(C1OP(=O)(O)O)OP(=O)(O)O)OP(=O)(O)O)OP(=O)(O)O)OP(=O)(O)O)OP(=O)(O)O.[Ca]
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| InChi Key |
WPEXVRDUEAJUGY-UHFFFAOYSA-B
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| InChi Code |
InChI=1S/C6H18O24P6.6Ca/c7-31(8,9)25-1-2(26-32(10,11)12)4(28-34(16,17)18)6(30-36(22,23)24)5(29-35(19,20)21)3(1)27-33(13,14)15;;;;;;/h1-6H,(H2,7,8,9)(H2,10,11,12)(H2,13,14,15)(H2,16,17,18)(H2,19,20,21)(H2,22,23,24);;;;;;/q;6*+2/p-12
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| Chemical Name |
hexacalcium;(2,3,4,5,6-pentaphosphonatooxycyclohexyl) phosphate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O: 8.7 mg/mL
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.3881 mL | 6.9406 mL | 13.8812 mL | |
| 5 mM | 0.2776 mL | 1.3881 mL | 2.7762 mL | |
| 10 mM | 0.1388 mL | 0.6941 mL | 1.3881 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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