| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg | |||
| Other Sizes |
| Targets |
L-Asparagine-d3 hydrate has no independent pharmacological target as a stable isotope internal standard. L-Asparagine itself is a non-essential amino acid that serves as a substrate for protein synthesis, a nitrogen carrier, and a precursor for the synthesis of other amino acids and nucleotides. It is involved in the metabolic control of cell functions in nerve and brain tissue via its role in the asparagine synthetase pathway.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
As an internal standard, L-Asparagine-d3 hydrate is not tested for in vitro pharmacological activity. It is added to biological samples to enable accurate quantification of L-asparagine levels. The non-labeled L-asparagine is an essential nutrient for certain cell types and can affect cell growth and survival. In cancer cells lacking asparagine synthetase, L-asparagine depletion by asparaginase is used as a therapy for acute lymphoblastic leukemia. |
| ln Vivo |
L-Asparagine-d3 hydrate is used as an internal standard and does not have independent in vivo activity. The non-labeled L-asparagine is involved in amino acid metabolism and protein synthesis. L-asparagine levels in plasma and tissues vary with physiological and pathological states. It is used in tracer studies to investigate nitrogen metabolism and the asparagine synthetase pathway in vivo.
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| Enzyme Assay |
For in vitro experiments using L-Asparagine-d3 hydrate as an internal standard, the compound is dissolved in an appropriate solvent (e.g., water, 0.1% formic acid in water) to prepare a stock solution at a concentration of 1-10 mg/mL. The internal standard is then added to biological samples (cell lysates, culture media, plasma) at a fixed concentration (e.g., 10-100 ng/mL) before protein precipitation and extraction. Samples are then analyzed by LC-MS/MS.
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| Cell Assay |
For cell-based studies, L-Asparagine-d3 hydrate is not used as a treatment but as an analytical reagent. Cells are cultured in standard medium (RPMI-1640 or DMEM with 10% FBS). For studies of asparagine metabolism, cells may be treated with L-asparaginase to deplete asparagine. After treatment, the internal standard L-Asparagine-d3 hydrate is added to cell lysates or culture supernatants. Following protein precipitation with methanol or acetonitrile, the samples are analyzed by LC-MS/MS to quantify L-asparagine levels.
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| Animal Protocol |
For in vivo pharmacokinetic or metabolomic studies, L-Asparagine-d3 hydrate is not administered to animals independently. Instead, it is used as an internal standard for quantifying L-asparagine in biological samples obtained from animals treated with experimental compounds. After collection of plasma, tissue homogenates, or other biological fluids, the internal standard is added to each sample at a fixed concentration, followed by protein precipitation, centrifugation, and LC-MS/MS analysis to determine absolute L-asparagine concentrations.
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| ADME/Pharmacokinetics |
L-Asparagine-d3 hydrate is an internal standard and has no PK parameters established independently. L-Asparagine is a naturally occurring amino acid with a plasma half-life of approximately 20-30 minutes in humans. It is distributed to all tissues and is metabolized by asparaginase to aspartate and ammonia. The deuterated version follows identical ADME properties and is used to calibrate analytical methods for the endogenous amino acid.
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| Toxicity/Toxicokinetics |
L-Asparagine is a naturally occurring non-essential amino acid with low toxicity. The LD50 in rodents is >5,000 mg/kg. The deuterated version is chemically identical except for isotopic substitution and exhibits the same safety profile. At high doses, L-asparagine may cause gastrointestinal disturbances. L-Asparagine-d3 hydrate is intended for research use only and should be handled with standard laboratory precautions. No specific toxicity data is available for the labeled compound.
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| References | |
| Additional Infomation |
L-Asparagine-d3 hydrate is not a drug but a deuterium-labeled stable isotope internal standard. It has no approved therapeutic status, no clinical trial history, and is not intended for human consumption. It is used exclusively as an analytical standard for the quantification of L-asparagine by GC- or LC-MS in pharmaceutical research, metabolomics, and amino acid analysis applications. Available with ≥95% purity and high isotopic enrichment (≥98 atom% D).
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| Molecular Formula |
C4H7D3N2O4
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|---|---|
| Molecular Weight |
150.133201122284
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| Exact Mass |
153.082
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| CAS # |
2483831-59-8
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| Related CAS # |
L-Asparagine;70-47-3
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| PubChem CID |
162642156
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| Appearance |
White to off-white solid powder
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
10
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| Complexity |
134
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| Defined Atom Stereocenter Count |
1
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| SMILES |
[C@@]([2H])(N)(C(=O)O)C([2H])([2H])C(=O)N.O
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| InChi Key |
RBMGJIZCEWRQES-HWSDRXMZSA-N
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| InChi Code |
InChI=1S/C4H8N2O3.H2O/c5-2(4(8)9)1-3(6)7;/h2H,1,5H2,(H2,6,7)(H,8,9);1H2/t2-;/m0./s1/i1D2,2D;
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| Chemical Name |
(2S)-2,4-diamino-2,3,3-trideuterio-4-oxobutanoic acid;hydrate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.6609 mL | 33.3045 mL | 66.6089 mL | |
| 5 mM | 1.3322 mL | 6.6609 mL | 13.3218 mL | |
| 10 mM | 0.6661 mL | 3.3304 mL | 6.6609 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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