| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
Monoethyl phthalate is an orally active PDX-1 activator. It targets aromatase (CYP19A1) and peroxisome proliferator-activated receptors (PPAR) to promote cell proliferation. As a urinary biomarker, it indicates the risk of thyroid cancer and benign nodules.
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|---|---|
| ln Vitro |
In vitro, monoethyl phthalate acts as a PDX-1 activator and targets aromatase and PPAR to promote cell proliferation. It is used in cell-based assays to study the effects of phthalate metabolites on endocrine function and cell proliferation. The compound's activity is assessed by measuring cell proliferation and gene expression changes.
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| ln Vivo |
In vivo, monoethyl phthalate is the primary hydrolytic metabolite of diethyl phthalate and is excreted in urine. It serves as a urinary biomarker of phthalate exposure, indicating the risk of thyroid cancer and benign nodules. It has been detected in various biological fluids and is used in reproductive toxicity research.
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| Enzyme Assay |
In vitro assays for monoethyl phthalate typically involve its use as a reference standard in LC-MS/MS methods for quantifying phthalate metabolites in urine or plasma. The compound is also used in cell-based assays to study its effects on PDX-1 activation, aromatase activity, and PPAR signaling. Cell proliferation is measured by MTT or BrdU incorporation assays.
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| Cell Assay |
For in vitro cell assays, cells are cultured and treated with monoethyl phthalate at concentrations ranging from 0.1-100 µM. Cell proliferation is assessed by MTT or BrdU incorporation assays. PDX-1 activation is measured by luciferase reporter assays. Aromatase activity is assessed by measuring estrogen production. PPAR signaling is evaluated by qPCR of target genes.
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| Animal Protocol |
For in vivo animal studies, monoethyl phthalate is typically administered to rodents via oral gavage to study its metabolism and excretion. Urine and blood samples are collected, and the compound is quantified by LC-MS/MS. Toxicological studies assess the effects of phthalate exposure on reproductive and endocrine function. The compound is also measured as a biomarker in exposure studies.
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| ADME/Pharmacokinetics |
Monoethyl phthalate (MW 194.18) has the molecular formula C10H10O4. It is a white, water-soluble powder. The compound is stable under recommended storage conditions. As the primary metabolite of diethyl phthalate, it is used as a urinary biomarker of phthalate exposure. It is a phthalic acid monoester and a benzoic acid derivative.
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| Toxicity/Toxicokinetics |
The compound is for research use only. Monoethyl phthalate has implications in reproductive toxicity research. As a metabolite of phthalates, it may have endocrine-disrupting effects at high concentrations. Standard laboratory safety precautions should be followed when handling this compound.
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| References | |
| Additional Infomation |
Monoethyl phthalate is a phthalic acid monoester formed by the condensation of a carboxyl group of phthalic acid with ethanol. It is a metabolite. It is both an ethyl ester and a monoethyl phthalate. Monoethyl phthalate (mEtP) is a metabolite or degradation product of diethyl phthalate and is used in perfumes, colognes, deodorants, soaps, shampoos, lotions, and other personal care products, especially those containing fragrances.
Monoethyl phthalate (CAS# 2306-33-4) is the primary hydrolytic metabolite of diethyl phthalate and a urinary biomarker of phthalate exposure. It has not been approved as a therapeutic agent. The compound is used in research on phthalate metabolism, reproductive toxicity, and endocrine disruption. It indicates the risk of thyroid cancer and benign nodules and is widely used in environmental and public health research. |
| Molecular Formula |
C10H10O4
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|---|---|
| Molecular Weight |
194.18
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| Exact Mass |
194.057
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| CAS # |
2306-33-4
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| Related CAS # |
Monoethyl phthalate-d4;1219806-03-7
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| PubChem CID |
75318
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| Appearance |
Colorless to light yellow liquid
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
339.6±25.0 °C at 760 mmHg
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| Melting Point |
46-48ºC
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| Flash Point |
135.3±16.7 °C
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| Vapour Pressure |
0.0±0.8 mmHg at 25°C
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| Index of Refraction |
1.547
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| LogP |
1.66
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
14
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| Complexity |
224
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CCOC(=O)C1=CC=CC=C1C(=O)O
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| InChi Key |
YWWHKOHZGJFMIE-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C10H10O4/c1-2-14-10(13)8-6-4-3-5-7(8)9(11)12/h3-6H,2H2,1H3,(H,11,12)
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| Chemical Name |
2-ethoxycarbonylbenzoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.1499 mL | 25.7493 mL | 51.4986 mL | |
| 5 mM | 1.0300 mL | 5.1499 mL | 10.2997 mL | |
| 10 mM | 0.5150 mL | 2.5749 mL | 5.1499 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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