| Size | Price | Stock | Qty |
|---|---|---|---|
| 500mg |
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| Other Sizes |
| Targets |
Chymotrypsinogen targets the same substrates as chymotrypsin, hydrolyzing peptide bonds with aromatic or large hydrophobic side chains. As a zymogen, chymotrypsinogen itself is inactive and must be converted to chymotrypsin to exert its proteolytic activity.
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|---|---|
| ln Vitro |
Chymotrypsinogen is used in the non-invasive determination of solid-state protein conformation using near infrared spectroscopy. It is also used in tissue repair due to its ability to resolve inflammatory symptoms.
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| ln Vivo |
Because chymotrypsinogen can alleviate inflammatory symptoms, it has been employed in tissue repair[2].
Chymotrypsinogen has been used in vivo in tissue repair due to its ability to resolve inflammatory symptoms. |
| Enzyme Assay |
The in vitro assay for Chymotrypsinogen typically involves its activation to chymotrypsin. Chymotrypsinogen is incubated with trypsin to generate active chymotrypsin. The activity of the generated chymotrypsin is then measured using a chromogenic substrate.
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| Cell Assay |
In vitro cellular assays are not typically performed with Chymotrypsinogen, as the compound is a zymogen used in biochemical research.
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| Animal Protocol |
Chymotrypsinogen has been used in vivo in tissue repair.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of Chymotrypsinogen are not relevant, as the compound is not used as a systemic therapeutic agent. It is used as a research reagent.
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| Toxicity/Toxicokinetics |
Toxicological data for Chymotrypsinogen are not extensively available, as the compound is used as a research reagent.
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| References | |
| Additional Infomation |
Chymotrypsinogen is the inactive precursor of chymotrypsin, a serine protease. It has a CAS number of 9035-75-0. No clinical trials or regulatory approvals have been reported.
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| CAS # |
9035-75-0
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|---|---|
| Appearance |
Typically exists as solid at room temperature
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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