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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
GABA-d2 is an isotopically labeled analog of GABA and does not have a specific pharmacological target. It is used as an analytical internal standard for the quantification of endogenous GABA in biological matrices. The deuterated form has identical chemical properties to native GABA but can be distinguished by mass spectrometry.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
GABA-d2 is not pharmacologically active in its own right; it serves as an analytical standard. It exhibits the same physicochemical properties as native GABA. The compound is used to correct for matrix effects, extraction efficiency, and instrument variability in quantitative analyses. GABA-d2 is used in cell culture experiments as an internal standard for measuring GABA concentrations. Cells are treated with GABA-d2 at known concentrations, and samples are analyzed by LC-MS/MS. The deuterated internal standard corrects for variations in sample preparation and instrument response. |
| ln Vivo |
In vivo, GABA-d2 is used as an internal standard for quantifying GABA concentrations in plasma, cerebrospinal fluid, or tissue samples. It is added to samples prior to analysis. The compound enables accurate measurement of endogenous GABA levels in pharmacokinetic and neurochemical studies.
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| Enzyme Assay |
Analytical method development for GABA quantification uses GABA-d2 as an internal standard. LC-MS/MS methods are validated using the deuterated standard. The compound exhibits identical chromatographic retention time and ionization efficiency as native GABA.
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| Cell Assay |
Cellular assays using GABA-d2 are limited to its use as an analytical internal standard. Cells are cultured and treated with GABA-d2 at known concentrations, followed by sample preparation and LC-MS/MS analysis. The compound is used to validate analytical methods for GABA quantification.
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| ADME/Pharmacokinetics |
As an internal standard, GABA-d2 exhibits identical pharmacokinetic behavior to native GABA. It is used in LC-MS/MS methods to measure GABA concentrations in plasma and cerebrospinal fluid. The deuterated compound enables accurate and reliable quantification of GABA in biological samples.
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| Toxicity/Toxicokinetics |
GABA-d2 is not a therapeutic agent and toxicity studies are not applicable. It is used in trace amounts as an analytical internal standard. Standard laboratory safety practices for handling chemical reference standards should be followed.
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| References |
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| Additional Infomation |
GABA-d2 is an analytical internal standard for GABA quantification in various biological matrices including plasma and cerebrospinal fluid. LC-MS/MS methods using GABA-d2 have been developed for clinical and research applications. The compound enables accurate measurement of GABA levels in pharmacokinetic and neurochemical studies.
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| Molecular Formula |
C4H7D2NO2
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|---|---|
| Molecular Weight |
105.13
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| Exact Mass |
105.076
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| CAS # |
67910-98-9
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| Related CAS # |
γ-Aminobutyric acid;56-12-2
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| PubChem CID |
57685050
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| Appearance |
White to off-white solid powder
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| Density |
1.132 g/cm3
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| Boiling Point |
247.985ºC at 760 mmHg
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| Melting Point |
195ºC (dec.)(lit.)
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| Flash Point |
103.778ºC
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| LogP |
0.51
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
7
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| Complexity |
62.7
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[2H]C([2H])(CCN)C(=O)O
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| InChi Key |
BTCSSZJGUNDROE-CBTSVUPCSA-N
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| InChi Code |
InChI=1S/C4H9NO2/c5-3-1-2-4(6)7/h1-3,5H2,(H,6,7)/i2D2
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| Chemical Name |
4-amino-2,2-dideuteriobutanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O: ≥ 50 mg/mL (475.60 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 9.5120 mL | 47.5602 mL | 95.1203 mL | |
| 5 mM | 1.9024 mL | 9.5120 mL | 19.0241 mL | |
| 10 mM | 0.9512 mL | 4.7560 mL | 9.5120 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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