| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| Other Sizes |
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| ln Vitro |
γ-Aminobutyric acid (30 μM) depolarizes cortical progenitor cells (E16 cells), causes inward currents in ventricular zone (VZ) cells, and inhibits DNA synthesis at a half-maximum response concentration of 5 μM [3]. Gamma-aminobutyric acid (1–5 μM; 18 h) increases cortical plate (cp) cell motility and inhibits migration, while G protein activation is involved in chemotactic signaling. GAD is expressed by cp neurons. Growth is restricted and cell cycle arrest occurs in the S phase due to the activation of GABA A receptors by γ-aminobutyric acid [5].
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|---|---|
| ln Vivo |
Mice's ability to sleep can be improved by gamma-aminobutyric acid (33.95, 102.25, 306.75 mg/kg; po; single dose) [6]. ? In rats (DEHP) exposed to di(2-ethylhexyl) phthalate, gamma-aminobutyric acid (1, 2, 4? mg/kg/d; oral; 30 days) decreases anxiety, improves food consumption, and repairs exposure-related damage [7].
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| Cell Assay |
Cell Migration Assay[4]
Cell Types: Cortical Plate (cp) Neuronal Tested Concentrations: 1-5 μM Incubation Duration: 18 hrs (hours) Experimental Results: Promotes motility via G protein activation and blocks attractants via GABAA receptor-mediated depolarization induced migration. |
| Animal Protocol |
Animal/Disease Models: Pathogen-free (SPF) Bagg albino (Balb/c) mice (18–20 g, 8 weeks old) [6]
Doses: 33.95, 102.25, 306.75 mg/kg single dose; administered at 20 mL/kg; Measured results in hrs (hrs (hours)): more effectively extend sleep time, increase sleep rate, and shorten sleep latency. Animal/Disease Models: SD (SD (Sprague-Dawley)) rats induced by DEHP (500 mg/kg) [7] Doses: 1, 2, 4 mg/kg Route of Administration: po (oral gavage); combined administration; 30 days Experimental Results: Treated with DEHP Levels of nitric oxide and nitric oxide synthase are diminished in rats. |
| References |
[1]. Chen S, et al. Effects of dietary gamma-aminobutyric acid supplementation on the intestinal functions in weaning piglets. Food Funct. 2019 Jan 2.
[2]. Okada R, et al. Gamma-aminobutyric acid (GABA)-mediated neural connections in the Drosophila antennal lobe. J Comp Neurol. 2009 May 1;514(1):74-91. |
| Molecular Formula |
C4H9NO2
|
|---|---|
| Molecular Weight |
103.12
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| Exact Mass |
103.0633
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| CAS # |
56-12-2
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| SMILES |
O=C(O)CCCN
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| Synonyms |
DF468; DF 468; Aminalon
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~50 mg/mL (~484.87 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (969.74 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication (<60°C).
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 9.6974 mL | 48.4872 mL | 96.9744 mL | |
| 5 mM | 1.9395 mL | 9.6974 mL | 19.3949 mL | |
| 10 mM | 0.9697 mL | 4.8487 mL | 9.6974 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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