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| Other Sizes |
| Targets |
Direct Red 80 targets collagen and amyloid proteins in histological samples. It stains collagen from solution in saturated aqueous picric acid and stains amyloid from an alkaline solution. The dye binds to the fibrillar structures of these proteins, enabling their visualization in tissue sections. Its polyazo structure allows it to interact with the protein structures through electrostatic and hydrophobic interactions, providing selective staining of collagen and amyloid deposits.
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| ln Vitro |
The staining of collagen in saturated aqueous picric acid solutions and the staining of amyloid in alkaline solutions are two crucial applications of Direct Red 80 (Sirius Red) in histopathology. The building is reddened [1]. Following Direct Red 80 labeling, collagen synthesis and distribution were assessed using confocal laser microscopy (CLM). Different forms of collagen do not affect Direct Red 80's effectiveness. The samples were diluted with 0.1% Direct Red 80 in saturated aqueous picric acid acid, rinsed in ddH2O, then prepped with 0.2% phosphomolybdic acid (PMA) solution for 40 minutes. After that, they were incubated for 30 minutes. Lastly, 0.01 M HCl was used to wash each sample for two minutes. Colorless cytoplasm is prepared using PMA, and non-specific staining is removed with HCl [1].
In vitro, Direct Red 80 is used for staining collagen and amyloid in tissue sections. It stains collagen from solution in saturated aqueous picric acid and stains amyloid from an alkaline solution. The dye is also used as a model compound in adsorption studies and has been utilized to compare the efficacy of textile dye removal in aqueous solutions. Its polyazo structure and anionic nature make it useful for various histopathological applications. |
| ln Vivo |
In vivo, Direct Red 80 has no direct therapeutic applications. It is primarily used in research and diagnostic settings for histological staining of collagen and amyloid in tissue sections. The dye does not release benzidine upon degradation, making it safer for laboratory use. It is used as a cardiac muscle stain and as a model compound in adsorption studies. Its primary in vivo application is in research involving tissue analysis and histopathology.
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| Enzyme Assay |
For non-cell-based receptor binding assays, Direct Red 80 is not typically used for receptor binding studies. Instead, its primary application is in histological staining. The dye can be evaluated for its binding affinity to collagen and amyloid proteins using spectrophotometric methods. Increasing concentrations of the dye are incubated with purified collagen or amyloid proteins, and the amount of bound dye is measured by absorbance at the dye's characteristic wavelength (typically around 500-600 nm). Binding affinity is calculated from binding isotherms using nonlinear regression analysis.
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| Cell Assay |
For in vitro cellular assays, Direct Red 80 is not typically used for cell-based functional assays. Instead, it is used for staining cells or tissue sections in histopathological applications. For collagen staining, tissue sections are incubated with Direct Red 80 in saturated aqueous picric acid solution. For amyloid staining, tissue sections are incubated with the dye in an alkaline solution. Stained sections are then examined under a microscope to visualize collagen fibers (red staining) or amyloid deposits (red staining). The intensity of staining can be quantified using image analysis software.
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| Animal Protocol |
For in vivo animal studies, Direct Red 80 is not typically used for therapeutic applications. It is used in research settings for tissue staining following animal sacrifice. Animals are euthanized, and tissues are collected, fixed, sectioned, and stained with Direct Red 80 for histological analysis. The dye is used to stain collagen and amyloid in tissue sections from various organs to assess fibrosis, amyloidosis, or other pathological conditions. The staining protocol follows standard histopathological procedures.
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| ADME/Pharmacokinetics |
Direct Red 80 has a molecular weight of 1373.05 g/mol and a molecular formula of C45H26N10Na6O21S6. It is a polyazo dye that does not release benzidine upon degradation. The dye is soluble in water and is typically used as a staining solution in histopathology. It is used as a cardiac muscle stain and as a model compound in adsorption studies. The compound should be stored according to the manufacturer's recommendations.
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| Toxicity/Toxicokinetics |
The toxicity profile of Direct Red 80 is favorable compared to many traditional direct dyes because it does not release benzidine upon degradation. Benzidine is a known carcinogen, and its absence from the degradation products of Direct Red 80 makes it safer for laboratory use. The compound is for research use only and not for human consumption. Standard safety precautions should be observed when handling the dye, including appropriate personal protective equipment.
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| References | |
| Additional Infomation |
Sirius Red F3B is an organosodium salt formed by the condensation of Sirius Red F3B (acid form) with six equivalents of sodium hydroxide. It is a polyazo dye primarily used for staining collagen and amyloid proteins. It can be used as both a fluorescent dye and a histological dye. Its molecular structure contains a Sirius Red F3B(6-) group.
Direct Red 80 (Sirius Red; Sirius Red) is a polyazo dye primarily used in staining methods for collagen and amyloid proteins. It stains collagen from solution in saturated aqueous picric acid and stains amyloid from an alkaline solution. The dye does not release benzidine upon degradation, making it safer than many traditional direct dyes. It has a molecular formula of C45H26N10Na6O21S6 and a molecular weight of 1373.05 g/mol. It is used as both a fluorescent dye and a histological dye. |
| Molecular Formula |
C45H26N10NA6O21S6
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|---|---|
| Molecular Weight |
1373.07
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| Exact Mass |
1371.9
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| CAS # |
2610-10-8
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| Related CAS # |
25188-41-4 (Parent)
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| PubChem CID |
75783
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| Appearance |
Brown to reddish brown solid powder
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| Density |
1.85 g/cm3
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| LogP |
14.765
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
29
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| Rotatable Bond Count |
10
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| Heavy Atom Count |
88
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| Complexity |
2790
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C1=CC(=CC=C1N=NC2=CC(=C(C=C2)N=NC3=C(C=C4C=C(C=CC4=C3O)NC(=O)NC5=CC6=CC(=C(C(=C6C=C5)O)N=NC7=C(C=C(C=C7)N=NC8=CC=C(C=C8)S(=O)(=O)[O-])S(=O)(=O)[O-])S(=O)(=O)[O-])S(=O)(=O)[O-])S(=O)(=O)[O-])S(=O)(=O)[O-].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+]
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| InChi Key |
YIQKLZYTHXTDDT-UHFFFAOYSA-H
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| InChi Code |
InChI=1S/C45H32N10O21S6.6Na/c56-43-33-13-5-27(17-23(33)19-39(81(71,72)73)41(43)54-52-35-15-7-29(21-37(35)79(65,66)67)50-48-25-1-9-31(10-2-25)77(59,60)61)46-45(58)47-28-6-14-34-24(18-28)20-40(82(74,75)76)42(44(34)57)55-53-36-16-8-30(22-38(36)80(68,69)70)51-49-26-3-11-32(12-4-26)78(62,63)64;;;;;;/h1-22,56-57H,(H2,46,47,58)(H,59,60,61)(H,62,63,64)(H,65,66,67)(H,68,69,70)(H,71,72,73)(H,74,75,76);;;;;;/q;6*+1/p-6
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| Chemical Name |
hexasodium;4-hydroxy-7-[[5-hydroxy-7-sulfonato-6-[[2-sulfonato-4-[(4-sulfonatophenyl)diazenyl]phenyl]diazenyl]naphthalen-2-yl]carbamoylamino]-3-[[2-sulfonato-4-[(4-sulfonatophenyl)diazenyl]phenyl]diazenyl]naphthalene-2-sulfonate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: < 1 mg/mL
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.7283 mL | 3.6415 mL | 7.2829 mL | |
| 5 mM | 0.1457 mL | 0.7283 mL | 1.4566 mL | |
| 10 mM | 0.0728 mL | 0.3641 mL | 0.7283 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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