| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
β adrenergic receptor
Not applicable for the deuterated internal standard. Unlabeled phenylethanolamine A is a beta-adrenergic receptor agonist that acts as a byproduct during the synthesis of ractopamine. It has been illegally used as a growth promoter in livestock in some countries. As a beta-agonist, it binds to and activates beta-adrenergic receptors, leading to increased muscle mass and decreased fat deposition in animals. |
|---|---|
| ln Vitro |
Phenylethanolamine A-d3 is not used for measuring intrinsic biological activity; its role is analytical. Unlabeled phenylethanolamine A acts as a beta-adrenergic receptor agonist, with pharmacological properties similar to other beta-agonists such as clenbuterol and ractopamine. It binds to beta1 and beta2 adrenergic receptors, activating downstream signaling pathways that promote protein synthesis and lipolysis.
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| ln Vivo |
Phenylethanolamine A-d3 itself is not used for in vivo activity studies. Unlabeled phenylethanolamine A, when administered to livestock (typically via feed), promotes lean muscle growth and reduces fat deposition, similar to other beta-agonists used in animal agriculture. However, its use is illegal in many countries due to concerns about residues in animal-derived food products and potential adverse effects in consumers.
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| Enzyme Assay |
Phenylethanolamine A-d3 is not used in non-cellular binding assays as a test compound. For binding studies of unlabeled phenylethanolamine A, a standard beta-adrenergic receptor binding assay is used. Membrane preparations from cells expressing human beta1 or beta2 adrenergic receptors are incubated with a radioligand (e.g., [3H]CGP-12177) and varying concentrations of the unlabeled compound. After incubation, bound and free radioligand are separated by filtration, and radioactivity is counted to determine binding affinity (Ki).
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| Cell Assay |
No standard cell-based protocol is applicable for the deuterated standard. For functional characterization of unlabeled phenylethanolamine A, CHO cells expressing human beta2-adrenergic receptors are used. Cells are treated with varying concentrations of the compound, and intracellular cAMP levels are measured by HTRF or ELISA. An increase in cAMP indicates successful activation of the beta-adrenergic receptor/Gs signaling pathway.
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| Animal Protocol |
Phenylethanolamine A-d3 is used as an internal standard in residue analysis rather than as a test compound in animal efficacy studies. A typical protocol for residue monitoring involves extracting phenylethanolamine A from animal tissues (e.g., liver, muscle) or feed using a QuEChERS method. The deuterated internal standard is added at the beginning of the extraction process to correct for recovery losses. Extracts are analyzed by LC-MS/MS for quantification.
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| ADME/Pharmacokinetics |
Phenylethanolamine A-d3 is chemically identical to the unlabeled analyte except for a +3 Da mass shift due to three deuterium atoms, resulting in virtually identical extraction recovery, chromatographic retention time, and ionization efficiency. It is stable under standard LC-MS conditions. Unlabeled phenylethanolamine A has a molecular weight of 344.4 g/mol and has been detected in animal-derived food products.
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| Toxicity/Toxicokinetics |
No direct toxicological data is provided for the deuterated standard, as it is used at trace analytical levels. Unlabeled phenylethanolamine A, as a beta-adrenergic agonist, can cause tachycardia, tremors, nervousness, and metabolic disturbances in exposed animals or humans. The presence of illegal residues in animal-derived foods is a public health concern, and regulatory authorities have established maximum residue limits (MRLs) for beta-agonists.
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| References | |
| Additional Infomation |
Phenylethanolamine A-d3 is an essential analytical reference standard for food safety monitoring. The compound is used by regulatory laboratories to detect and quantify illegal use of the beta-agonist phenylethanolamine A in livestock production. Phenylethanolamine A is known to be a byproduct of ractopamine synthesis and has been found as an adulterant in animal feed. The deuterated internal standard enables accurate, regulatory-compliant analysis by LC-MS/MS.
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| Molecular Formula |
C19H21D3N2O4
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|---|---|
| Molecular Weight |
347.42
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| Exact Mass |
347.192
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| CAS # |
2507994-61-6
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| Related CAS # |
Phenylethanolamine A;1346746-81-3
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| PubChem CID |
146673161
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| Appearance |
White to off-white solid powder
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| LogP |
3.2
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
8
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| Heavy Atom Count |
25
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| Complexity |
385
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O(C([2H])([2H])[2H])C1C=CC(=CC=1)C(CNC(C)CCC1C=CC(N(=O)=O)=CC=1)O
|
| InChi Key |
DVUFPRMEKXKECP-BMSJAHLVSA-N
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| InChi Code |
InChI=1S/C19H24N2O4/c1-14(3-4-15-5-9-17(10-6-15)21(23)24)20-13-19(22)16-7-11-18(25-2)12-8-16/h5-12,14,19-20,22H,3-4,13H2,1-2H3/i2D3
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| Chemical Name |
2-[4-(4-nitrophenyl)butan-2-ylamino]-1-[4-(trideuteriomethoxy)phenyl]ethanol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.8784 mL | 14.3918 mL | 28.7836 mL | |
| 5 mM | 0.5757 mL | 2.8784 mL | 5.7567 mL | |
| 10 mM | 0.2878 mL | 1.4392 mL | 2.8784 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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