| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
| Targets |
Guanfacine-13C,15N3 targets the same pathways as the parent compound guanfacine. Guanfacine is an orally active noradrenergic α2A agonist that is selective for the α2A receptor subtype. Guanfacine has hypotensive and sedative effects. The 13C and 15N labeling does not alter the target specificity, as the isotopic substitution does not affect receptor binding.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
In vitro, Guanfacine-13C,15N3 is not used for biological activity assays but as an internal standard in analytical chemistry. Its utility lies in its ability to serve as a quantitative tracer for the detection and quantification of guanfacine in biological samples using mass spectrometry. The 13C and 15N labeling provides a distinct mass shift, allowing for accurate quantification and correction for matrix effects. |
| ln Vivo |
In vivo, Guanfacine-13C,15N3 is not administered for therapeutic purposes. Its primary use is as an internal standard in analytical studies to quantify guanfacine levels in biological samples. Guanfacine itself is used for the research of a variety of prefrontal cortex (PFC) cognitive disorders, including Tourette's syndrome and attention deficit hyperactivity disorder (ADHD).
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| Enzyme Assay |
For non-cell-based assays, Guanfacine-13C,15N3 is used as an analytical standard. Its identity and purity are confirmed using techniques such as NMR, HPLC, and mass spectrometry. It is not used in receptor binding assays as it is not intended for pharmacological study. Its role is to serve as a quantitative tracer in analytical method development.
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| Cell Assay |
For in vitro cellular assays, Guanfacine-13C,15N3 is not typically used, as it is an analytical standard. However, it may be used in cell culture media as a part of sample preparation for LC-MS/MS analysis to quantify guanfacine uptake or metabolism. The compound is added to biological samples at a known concentration to serve as an internal standard, correcting for variability during sample processing and analysis.
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| Animal Protocol |
For in vivo animal studies, Guanfacine-13C,15N3 is used as an internal standard for the quantification of guanfacine in pharmacokinetic and metabolic studies. Blood or tissue samples collected from animals dosed with guanfacine are spiked with a known amount of Guanfacine-13C,15N3. The samples are then processed and analyzed by LC-MS/MS. The ratio of the signal of the analyte to that of the internal standard is used to calculate the concentration of the analyte.
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| ADME/Pharmacokinetics |
Guanfacine-13C,15N3 has a molecular weight of 250.07 and a molecular formula of C813CH9Cl215N3O. It is a stable isotope-labeled compound. The compound is a controlled substance. It is for research use only and is not intended for human consumption. The compound should be stored under recommended conditions.
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| Toxicity/Toxicokinetics |
The toxicity profile of Guanfacine-13C,15N3 is not applicable, as it is used as an analytical standard at very low concentrations. The 13C and 15N labeling is not expected to introduce new toxicities, as these are stable, non-radioactive isotopes. The compound is not intended for human consumption. Standard safety precautions should be observed when handling the compound.
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| References |
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| Additional Infomation |
Guanfacine-13C,15N3 is the 13C and 15N labeled form of Guanfacine. Guanfacine is an orally active noradrenergic α2A agonist used for the research of prefrontal cortex cognitive disorders, including Tourette's syndrome and ADHD. Guanfacine-13C,15N3 is intended for use as an internal standard for the quantification of guanfacine by GC- or LC-MS. It is available for research purposes only.
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| Molecular Formula |
C813CH9CL215N3O
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|---|---|
| Molecular Weight |
250.07
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| Exact Mass |
249.007
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| CAS # |
1189924-28-4
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| Related CAS # |
Guanfacine;29110-47-2
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| PubChem CID |
46780454
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| Appearance |
Typically exists as solid at room temperature
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| LogP |
3.185
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
1
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
15
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| Complexity |
256
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[15NH]=[13C]([15NH2])[15NH]C(CC1=C(Cl)C=CC=C1Cl)=O
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| InChi Key |
INJOMKTZOLKMBF-DATIIYMNSA-N
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| InChi Code |
InChI=1S/C9H9Cl2N3O/c10-6-2-1-3-7(11)5(6)4-8(15)14-9(12)13/h1-3H,4H2,(H4,12,13,14,15)/i9+1,12+1,13+1,14+1
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| Chemical Name |
N-[bis(15N)(azanyl)(113C)methylidene]-2-(2,6-dichlorophenyl)acetamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.9989 mL | 19.9944 mL | 39.9888 mL | |
| 5 mM | 0.7998 mL | 3.9989 mL | 7.9978 mL | |
| 10 mM | 0.3999 mL | 1.9994 mL | 3.9989 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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