| Size | Price | Stock | Qty |
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| 100mg |
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| 500mg |
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| 1g |
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| Other Sizes |
| Targets |
Cytidine diphosphate targets metabolic enzymes involved in phospholipid biosynthesis. It acts as a carrier for phosphocholine, forming CDP-choline, which is a precursor for phosphatidylcholine synthesis. It also serves as a carrier for diacylglycerol in the synthesis of phosphatidylinositol. As a metabolite, it does not target a specific receptor but is a substrate for multiple enzymes in lipid metabolism.
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| ln Vitro |
In vitro, Cytidine diphosphate is used as a substrate in enzymatic assays for enzymes involved in phospholipid synthesis, such as choline-phosphate cytidylyltransferase (CCT). It is also used in studies of lipid metabolism and as a reference standard in analytical chemistry. It is not typically used for cell-based functional assays but is a critical component in biochemical studies of phospholipid biosynthesis.
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| ln Vivo |
In vivo, Cytidine diphosphate is an essential intermediate in the biosynthesis of phospholipids, which are major components of cell membranes. It plays a critical role in the synthesis of phosphatidylcholine, a major membrane phospholipid, and other lipids. It is an endogenous metabolite found in all living cells and is an Escherichia coli and mouse metabolite.
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| Enzyme Assay |
Cytidine diphosphate can be used in non-cell-based assays as a substrate for enzymes like choline-phosphate cytidylyltransferase. Enzyme activity is measured by monitoring the formation of CDP-choline from CTP and phosphocholine. The compound is also used as an analytical standard for the identification and quantification of nucleotides in biological samples using techniques like HPLC and mass spectrometry.
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| Cell Assay |
For in vitro cellular assays, Cytidine diphosphate is not typically used as a pharmacological agent. It may be used in studies of lipid metabolism, where cells are treated with the compound or its precursors and the synthesis of phospholipids is measured. However, its primary use is as a research tool in biochemical assays rather than as a modulator of cell function.
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| Animal Protocol |
For in vivo animal studies, Cytidine diphosphate is not typically administered for therapeutic purposes. It may be used in metabolic tracing studies where isotopically labeled CDP is administered to track phospholipid synthesis. However, its primary use is as a research tool in biochemistry and cell biology.
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| ADME/Pharmacokinetics |
Cytidine diphosphate has a molecular weight of 403.18 and a molecular formula of C9H15N3O11P2. It is a white crystalline powder. The compound is soluble in water. It should be stored at -20°C for long-term stability. It is supplied with a purity of ≥98%. It is for research use only and is not intended for human consumption.
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| Toxicity/Toxicokinetics |
The toxicity profile of Cytidine diphosphate is not applicable, as it is an endogenous metabolite. It is not considered toxic. The compound is for research use only and is not intended for human consumption. Standard safety precautions should be observed when handling the compound.
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| References | |
| Additional Infomation |
Cytosine diphosphate (CDP) is a pyrimidine ribonucleotide 5'-bisphosphate with cytosine as the nucleobase. It is a metabolite of Escherichia coli and mice. It is a pyrimidine ribonucleotide 5'-bisphosphate and a cytosine 5'-phosphate. It is the conjugate acid of CDP(3-). Cytosine-5'-bisphosphate has been reported to exist in humans, and relevant data are available. CDP is a metabolite found or produced in Saccharomyces cerevisiae. Cytosine 5'-(trihydrodiphosphate). A cytosine nucleotide whose sugar moiety is esterified with two phosphate groups. Synonyms: CRPP; cytosine pyrophosphate.
Cytidine diphosphate (CDP, CAS 63-38-7) is a nucleoside diphosphate that serves as a carrier molecule for phosphocholine, diacylglycerol, and other molecules during phospholipid synthesis. It is a pyrimidine ribonucleoside 5'-diphosphate having cytosine as the nucleobase. It is an endogenous metabolite and a key intermediate in phospholipid biosynthesis. It is available for research purposes only. |
| Molecular Formula |
C9H15N3O11P2
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|---|---|
| Molecular Weight |
403.18
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| Exact Mass |
403.018
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| CAS # |
63-38-7
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| Related CAS # |
Cytidine 5'-diphosphate trisodium salt;34393-59-4
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| PubChem CID |
6132
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| Appearance |
Typically exists as solid at room temperature
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| Density |
2.4±0.1 g/cm3
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| Boiling Point |
767.4±70.0 °C at 760 mmHg
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| Flash Point |
417.9±35.7 °C
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| Vapour Pressure |
0.0±5.9 mmHg at 25°C
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| Index of Refraction |
1.789
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| LogP |
-4.25
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| Hydrogen Bond Donor Count |
6
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| Hydrogen Bond Acceptor Count |
11
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
25
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| Complexity |
690
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| Defined Atom Stereocenter Count |
4
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| SMILES |
O[C@@H]([C@H]([C@H](N1C(N=C(C=C1)N)=O)O2)O)[C@H]2COP(O)(OP(O)(O)=O)=O
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| InChi Key |
ZWIADYZPOWUWEW-XVFCMESISA-N
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| InChi Code |
InChI=1S/C9H15N3O11P2/c10-5-1-2-12(9(15)11-5)8-7(14)6(13)4(22-8)3-21-25(19,20)23-24(16,17)18/h1-2,4,6-8,13-14H,3H2,(H,19,20)(H2,10,11,15)(H2,16,17,18)/t4-,6-,7-,8-/m1/s1
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| Chemical Name |
[(2R,3S,4R,5R)-5-(4-amino-2-oxopyrimidin-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphono hydrogen phosphate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4803 mL | 12.4014 mL | 24.8028 mL | |
| 5 mM | 0.4961 mL | 2.4803 mL | 4.9606 mL | |
| 10 mM | 0.2480 mL | 1.2401 mL | 2.4803 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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