| Size | Price | Stock | Qty |
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| Targets |
D-Sorbitol-d8 does not have a specific pharmacological target. Instead, it is used as an analytical tool. As a stable isotope-labeled internal standard, its purpose is to mimic the chemical behavior of the unlabeled D-sorbitol during sample preparation and analysis, while being distinguishable by mass spectrometry due to its mass shift. In metabolic tracing studies, it follows the same biochemical pathways as unlabeled sorbitol, allowing researchers to track its metabolic fate without using radioactive isotopes.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
D-Sorbitol-d8 is not used in conventional biological activity assays. Its utility is purely analytical, functioning as an internal standard to correct for variability during sample processing and to enable precise quantification of endogenous sorbitol. By co-eluting with the analyte and behaving identically during extraction and ionization, it accounts for matrix effects and ion suppression in mass spectrometry. Its high isotopic purity ensures accurate correction for recovery and ionization efficiency. |
| ln Vivo |
D-Sorbitol-d8 is not administered for therapeutic purposes. Its primary use is as an internal standard in analytical studies to quantify sorbitol levels in biological samples, and as a tracer to map polyol pathway flux (sorbitol → fructose) in vivo. By using the deuterated analog, researchers can accurately measure the concentration of endogenous or exogenously administered sorbitol in pharmacokinetic, metabolic, and flux studies.
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| Enzyme Assay |
For non-cell-based assays, D-Sorbitol-d8 is used as an analytical standard. Its identity and purity are confirmed using techniques such as NMR and mass spectrometry. The compound is typically supplied with a chemical purity of ≥99% (CP) and an isotopic enrichment of ≥98 atom% D. It is not used in receptor binding assays. Its role is to serve as a quantitative internal standard in the development and validation of bioanalytical methods for quantifying sorbitol in biological fluids, foods, and other matrices.
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| Cell Assay |
For in vitro cellular assays, D-Sorbitol-d8 is not typically used as a pharmacological agent. However, it can be incorporated into cell culture media as part of sample preparation for LC-MS/MS analysis to quantify sorbitol uptake or metabolism. The compound is added to biological samples (e.g., cell lysates, culture media) at a known concentration to serve as an internal standard, correcting for variability during sample processing and analysis. It can also be used to study polyol pathway activity in cultured cells.
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| Animal Protocol |
For in vivo animal studies, D-Sorbitol-d8 is used as an internal standard and metabolic tracer. In pharmacokinetic and metabolic studies, blood or tissue samples collected from animals are spiked with a known amount of the compound. The samples are then processed and analyzed by LC-MS/MS. The ratio of the signal of the analyte to that of the internal standard is used to calculate the concentration of the analyte. As a tracer, it can be administered to animals to map the polyol pathway and study sorbitol dehydrogenase activity.
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| ADME/Pharmacokinetics |
D-Sorbitol-d8 has a molecular weight of 190.22 g/mol and a molecular formula of C6H6D8O6. It is chemically identical in structure and reactivity to unlabeled D-sorbitol (CAS 50-70-4). The compound is commercially available with high isotopic purity and is stable for research use. The deuterium atoms provide a +8 Da mass shift, enabling clear separation from unlabeled sorbitol in mass spectrometry. D-Sorbitol-d8 is typically stored at room temperature or -20°C for long-term stability.
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| Toxicity/Toxicokinetics |
The toxicity profile of D-Sorbitol-d8 is not applicable, as it is used as an analytical standard and tracer at very low concentrations. The deuterium substitution is not expected to introduce new toxicities, as deuterium is a stable, non-radioactive isotope of hydrogen generally considered safe for research applications. The compound is not intended for human consumption. Standard safety precautions should be observed when handling the compound.
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| References |
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| Additional Infomation |
D-Sorbitol-d8 (CAS 287962-59-8) is a deuterium-labeled form of D-Sorbitol, a sugar alcohol also known as D-Glucitol. It is used as an internal standard for the accurate quantification of unlabeled sorbitol in biological matrices via LC-MS/MS and GC-MS. The compound is synthesized via the catalytic hydrogenation of D-Glucose-d8. Its primary applications are in clinical biomarker assays, metabolic research, and quality control for 'sugar-free' regulatory claims. It is not approved for therapeutic use and is available for research purposes only.
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| Molecular Formula |
C6H14O6
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| Molecular Weight |
190.221056461334
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| Exact Mass |
190.129
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| CAS # |
287962-59-8
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| Related CAS # |
D-Sorbitol;50-70-4
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| PubChem CID |
119081674
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| Appearance |
White to off-white solid powder
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| LogP |
-3.1
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| Hydrogen Bond Donor Count |
6
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
12
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| Complexity |
105
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| Defined Atom Stereocenter Count |
4
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| SMILES |
C([2H])([2H])(O)[C@@]([2H])(O)[C@@]([2H])(O)[C@]([2H])(O)[C@@]([2H])(O)C([2H])([2H])O
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| InChi Key |
FBPFZTCFMRRESA-IUAGDLHUSA-N
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| InChi Code |
InChI=1S/C6H14O6/c7-1-3(9)5(11)6(12)4(10)2-8/h3-12H,1-2H2/t3-,4-,5-,6-/m1/s1/i1D2,2D2,3D,4D,5D,6D
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| Chemical Name |
(2R,3R,4R,5R)-1,1,2,3,4,5,6,6-octadeuteriohexane-1,2,3,4,5,6-hexol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.2571 mL | 26.2854 mL | 52.5707 mL | |
| 5 mM | 1.0514 mL | 5.2571 mL | 10.5141 mL | |
| 10 mM | 0.5257 mL | 2.6285 mL | 5.2571 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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