| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| 250mg |
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| Other Sizes |
| Targets |
D-Glucose-13C-3 does not have a specific pharmacological receptor target. As a stable isotope-labeled compound, it is used as a tracer in metabolic studies. D-Glucose is a key metabolite involved in glycolysis, the pentose phosphate pathway, and other metabolic pathways. The 13C labeling at the C-3 position allows for the tracking of carbon atoms through these pathways.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
In vitro, D-Glucose-13C-3 is not used for biological activity assays. Its primary utility is as a tracer in metabolic studies to track glucose metabolism in cells. Cells are cultured in media containing the labeled glucose, and the incorporation of 13C into downstream metabolites is analyzed. This allows for the study of glycolysis, the TCA cycle, and other metabolic pathways. |
| ln Vivo |
In vivo, D-Glucose-13C-3 is used as a metabolic tracer to study glucose metabolism and insulin sensitivity. By administering the labeled compound and measuring the incorporation of 13C into metabolites using mass spectrometry, researchers can map glucose flux in various tissues. It is used in metabolic studies to understand energy metabolism and related disorders.
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| Enzyme Assay |
For non-cell-based assays, D-Glucose-13C-3 is used as an analytical standard. Its identity and purity are confirmed using techniques such as NMR and mass spectrometry. It is not used in receptor binding assays. Its role is to serve as a quantitative tracer in metabolic studies, enabling the tracking of carbon atoms through glucose metabolic pathways.
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| Cell Assay |
For in vitro cellular assays, cells are cultured in media containing D-Glucose-13C-3 instead of unlabeled glucose. The cells metabolize the labeled glucose through various pathways. After a defined incubation period, cells are harvested, and metabolites are extracted. The incorporation of 13C into metabolites is analyzed using LC-MS/MS or GC-MS to determine metabolic fluxes and pathway activities.
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| Animal Protocol |
For in vivo animal studies, D-Glucose-13C-3 can be administered to rodents via oral gavage or intravenous injection. Blood or tissue samples are collected at various time points post-administration. The samples are processed and analyzed by mass spectrometry to track the distribution and metabolism of the labeled glucose. This allows for the study of glucose metabolism and insulin sensitivity in vivo.
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| ADME/Pharmacokinetics |
D-Glucose-13C-3 has a molecular weight of 181.15 g/mol and a molecular formula of C6H12O6 (with one 13C atom). It is a 13C-labeled compound. The compound is soluble in water. It should be stored at room temperature for short-term use or at -20°C for long-term stability. It is for research use only and is not intended for human consumption.
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| Toxicity/Toxicokinetics |
The toxicity profile of D-Glucose-13C-3 is not applicable, as it is used as a metabolic tracer at very low concentrations. The 13C labeling is not expected to introduce new toxicities, as carbon-13 is a stable, non-radioactive isotope. The compound is not intended for human consumption. Standard safety precautions should be observed when handling the compound.
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| References | |
| Additional Infomation |
D-Glucose-13C-3 (CAS 105931-74-6) is the 13C-labeled form of D-Glucose, where the carbon-3 position is enriched with the 13C isotope. It is used as a tracer in metabolic studies to track glucose metabolism, glycolysis, and the TCA cycle. It is available for research purposes only.
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| Molecular Formula |
C513CH12O6
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|---|---|
| Molecular Weight |
181.15
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| Exact Mass |
181.067
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| CAS # |
105931-74-6
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| Related CAS # |
D-Glucose;50-99-7
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| PubChem CID |
12285870
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| Appearance |
Typically exists as solid at room temperature
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| Density |
1.732g/cm3
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| Melting Point |
150-152ºC(lit.)
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| Index of Refraction |
1.635
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| LogP |
-2.6
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| Hydrogen Bond Donor Count |
5
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
12
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| Complexity |
151
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| Defined Atom Stereocenter Count |
4
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| SMILES |
C([C@@H]1[C@H]([C@@H]([13C@H](C(O)O1)O)O)O)O
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| InChi Key |
WQZGKKKJIJFFOK-UKLRSMCWSA-N
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| InChi Code |
InChI=1S/C6H12O6/c7-1-2-3(8)4(9)5(10)6(11)12-2/h2-11H,1H2/t2-,3-,4+,5-,6?/m1/s1/i5+1
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| Chemical Name |
(3R,4S,5S,6R)-6-(hydroxymethyl)(313C)oxane-2,3,4,5-tetrol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.5203 mL | 27.6014 mL | 55.2029 mL | |
| 5 mM | 1.1041 mL | 5.5203 mL | 11.0406 mL | |
| 10 mM | 0.5520 mL | 2.7601 mL | 5.5203 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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