| Size | Price | Stock | Qty |
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| 100mg |
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| 500mg |
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| Other Sizes |
| Targets |
5-AAM-2-CP is a metabolite of Acetamiprid, which targets nicotinic acetylcholine receptors (nAChRs). As a metabolite, it may not have significant direct activity at nAChRs, but it is used as a biomarker for Acetamiprid exposure. Acetamiprid itself acts as an agonist of nAChRs, leading to neurotoxic effects in insects.
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| ln Vitro |
In vitro, 5-AAM-2-CP is the hydrolyzed product of Acetamiprid and can be tested in both blood and urine. It is not typically used for biological activity assays. Its primary utility is as a biomarker for Acetamiprid exposure in toxicological and environmental studies. It is used as an analytical standard for the quantification of Acetamiprid metabolites.
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| ln Vivo |
5-AAM-2-CP, the hydrolyzed N-desmethylacetamiprid product (5-(N-acetylaminomethyl)-2-chloropyridine), Both blood and urine can undergo the metabolism of acetamiprid to 5-AAM-2-CP[1].
In vivo, 5-AAM-2-CP is a major metabolite of Acetamiprid found in blood and urine. Its levels are used as a biomarker for Acetamiprid exposure in humans and animals. It is not used for therapeutic purposes. Its primary relevance is in toxicology and environmental monitoring. |
| Enzyme Assay |
For non-cell-based assays, 5-AAM-2-CP is used as an analytical standard. Its identity and purity are confirmed using techniques such as NMR, HPLC, and mass spectrometry. It is not used in receptor binding assays. Its role is to serve as a reference standard for the identification and quantification of Acetamiprid metabolites in biological and environmental samples.
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| Cell Assay |
For in vitro cellular assays, 5-AAM-2-CP is not typically used for cell-based functional assays. It may be used in toxicological studies to assess the effects of Acetamiprid metabolites on cells. However, its primary use is as an analytical standard and a biomarker rather than a pharmacological tool.
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| Animal Protocol |
For in vivo animal studies, 5-AAM-2-CP is not typically administered for therapeutic purposes. It may be measured in biological samples from animals exposed to Acetamiprid to assess its metabolism and elimination. Its levels in blood and urine serve as biomarkers of exposure.
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| ADME/Pharmacokinetics |
5-AAM-2-CP has a molecular weight of 184.62 and a molecular formula of C8H9ClN2O. It has a purity of 99.58%. The compound is soluble in DMSO at 50 mg/mL (270.83 mM). It should be stored as a powder at -20°C for up to 3 years or in solvent at -80°C for 1 year. It is for research use only and is not intended for human consumption.
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| Toxicity/Toxicokinetics |
The toxicity profile of 5-AAM-2-CP has not been extensively reported. As a metabolite of a neonicotinoid insecticide, its toxicity is related to the parent compound's insecticidal activity. The compound is for research use only and is not intended for human consumption. Standard safety precautions should be observed when handling the compound.
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| References |
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| Additional Infomation |
5-AAM-2-CP (CAS 175424-74-5) is one of the major metabolites of Acetamiprid, a neonicotinoid insecticide used worldwide. Acetamiprid is a nAChR agonist. 5-AAM-2-CP is the hydrolyzed product of Acetamiprid and can be tested in both blood and urine. It is used as a biomarker for Acetamiprid exposure. It is available for research purposes only.
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| Molecular Formula |
C8H9CLN2O
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|---|---|
| Molecular Weight |
184.62
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| Exact Mass |
184.04
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| CAS # |
175424-74-5
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| PubChem CID |
11008612
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| Appearance |
Off-white to light yellow solid powder
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| LogP |
1.762
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| tPSA |
41.99
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
12
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| Complexity |
163
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CC(=O)NCC1=CN=C(C=C1)Cl
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| InChi Key |
PKLYKZAYVXYVQX-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C8H9ClN2O/c1-6(12)10-4-7-2-3-8(9)11-5-7/h2-3,5H,4H2,1H3,(H,10,12)
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| Chemical Name |
N-[(6-chloropyridin-3-yl)methyl]acetamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ~100 mg/mL (~541.7 mM; with ultrasonication)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (13.54 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (13.54 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (13.54 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.4165 mL | 27.0827 mL | 54.1653 mL | |
| 5 mM | 1.0833 mL | 5.4165 mL | 10.8331 mL | |
| 10 mM | 0.5417 mL | 2.7083 mL | 5.4165 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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