| Size | Price | Stock | Qty |
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| 10mg |
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| 25mg |
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| Targets |
Acetylcholine-d9 chloride targets nicotinic acetylcholine receptors (nAChRs). As a cholinergic agonist, it mimics the action of endogenous acetylcholine. It stimulates these receptors, modulating the activity of dopaminergic neurons. The deuterium labeling does not alter its binding affinity or pharmacological activity, making it a perfect tracer for the unlabeled molecule.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
In vitro, Acetylcholine-d9 chloride functions as a potent cholinergic agonist, similar to its non-deuterated counterpart. It stimulates nAChRs and modulates the activity of dopaminergic neurons. Its primary utility is as an internal standard for precise quantification in mass spectrometry-based assays. |
| ln Vivo |
In vivo, Acetylcholine-d9 chloride is not administered for therapeutic purposes. Its use is primarily as an analytical tool. When spiked into biological samples (e.g., plasma, brain homogenates), it serves as an internal standard to accurately quantify endogenous acetylcholine levels. This is essential for understanding cholinergic neurotransmission in health and disease.
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| Enzyme Assay |
Non-cell-based assays using Acetylcholine-d9 chloride primarily involve its function as an internal standard in mass spectrometry (LC-MS/MS). A known amount of the deuterated compound is added to biological samples. The ratio of the signal from the endogenous acetylcholine to the signal from the labeled standard is used to calculate the precise concentration of the analyte, correcting for any losses during sample preparation and analysis.
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| Cell Assay |
For in vitro cellular assays, Acetylcholine-d9 chloride can be used to study cholinergic signaling. Cells expressing nAChRs can be treated with the compound, and its effects on downstream signaling pathways can be measured. However, its most common in vitro application is as a standard in neurochemical analysis of cell culture media or lysates.
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| Animal Protocol |
In vivo animal studies utilize Acetylcholine-d9 chloride as an internal standard for pharmacokinetic and neurochemical studies. For example, microdialysis samples collected from the brains of animals can be spiked with the labeled compound to accurately quantify the concentration of acetylcholine released during specific behavioral or pharmacological manipulations.
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| ADME/Pharmacokinetics |
Acetylcholine-d9 chloride has a molecular weight and formula as described for its deuterated forms. It is typically supplied as a solid with high purity (e.g., 99.0%). It is intended for research use only and not for human consumption. It should be stored at -20°C, as recommended for similar compounds.
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| Toxicity/Toxicokinetics |
The toxicity profile of Acetylcholine-d9 chloride is not detailed. As a research chemical used in minute quantities as an analytical standard, its toxicity is not a primary concern. However, as a cholinergic agonist, it could have pharmacological effects if administered in significant amounts. It should be handled with standard laboratory safety practices.
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| References |
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| Additional Infomation |
Acetylcholine-d9 chloride (CAS#: 344298-95-9) is a deuterated internal standard for the neurotransmitter acetylcholine. It is essential for accurate quantification of acetylcholine in complex biological matrices, supporting research in neurochemistry, neuropharmacology, and drug development. Its use allows for precise measurement of cholinergic system function.
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| Molecular Formula |
C7H7D9CLNO2
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|---|---|
| Molecular Weight |
190.72
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| Exact Mass |
190.143
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| CAS # |
344298-95-9
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| Related CAS # |
Acetylcholine chloride;60-31-1
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| PubChem CID |
45038081
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| Appearance |
White to off-white solid powder
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| Melting Point |
149-152ºC
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
11
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| Complexity |
115
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[2H]C([2H])([2H])[N+](CCOC(=O)C)(C([2H])([2H])[2H])C([2H])([2H])[2H].[Cl-]
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| InChi Key |
JUGOREOARAHOCO-WWMMTMLWSA-M
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| InChi Code |
InChI=1S/C7H16NO2.ClH/c1-7(9)10-6-5-8(2,3)4;/h5-6H2,1-4H3;1H/q+1;/p-1/i2D3,3D3,4D3;
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| Chemical Name |
2-acetyloxyethyl-tris(trideuteriomethyl)azanium;chloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
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| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.2433 mL | 26.2164 mL | 52.4329 mL | |
| 5 mM | 1.0487 mL | 5.2433 mL | 10.4866 mL | |
| 10 mM | 0.5243 mL | 2.6216 mL | 5.2433 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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