| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
Semicarbazide-sensitive amine oxidase (SSAO).
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|---|---|
| ln Vitro |
Aminoacetone hydrochloride is a substrate for SSAO, an enzyme that plays a role in the metabolism of biogenic amines. Its activity is measured by monitoring the enzymatic conversion of aminoacetone, which can be used to determine SSAO activity in various biological samples. This makes it a useful reagent for studying SSAO function and its involvement in physiological and pathological processes.
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| ln Vivo |
As an endogenous metabolite, Aminoacetone is involved in normal metabolic pathways. Its levels can be affected by various physiological and pathological conditions. It is used in research to study metabolic disorders and the role of SSAO in diseases such as diabetes and cardiovascular disease.
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| Enzyme Assay |
In vitro enzyme assays are performed to measure the activity of SSAO using Aminoacetone hydrochloride as a substrate. The enzyme is incubated with the substrate, and the production of the reaction product (e.g., methylglyoxal and ammonia) is measured using spectrophotometric or fluorometric methods. The rate of product formation reflects SSAO activity.
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| Cell Assay |
For in vitro cell-based assays, cells or tissues expressing SSAO are incubated with Aminoacetone hydrochloride. The metabolism of the substrate by the enzyme is monitored, and the effects of various inhibitors or modulators on SSAO activity can be assessed. This allows researchers to study the regulation of SSAO in a cellular context.
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| Animal Protocol |
In vivo studies may involve the administration of Aminoacetone hydrochloride to animal models to study its metabolic effects and the role of SSAO in disease. However, as an endogenous metabolite, it is often used as a biomarker rather than a therapeutic agent. Its levels in biological fluids can be measured to assess metabolic status.
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| ADME/Pharmacokinetics |
No specific pharmacokinetic data are available for Aminoacetone hydrochloride. As an endogenous metabolite, it is rapidly metabolized and cleared from the body. Its levels are tightly regulated by metabolic pathways. For research purposes, its stability in biological samples and its metabolism are key considerations.
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| Toxicity/Toxicokinetics |
No specific toxicity data are available. As an endogenous compound, it is generally well-tolerated at physiological concentrations. However, at high concentrations, it may have toxic effects due to the production of reactive metabolites like methylglyoxal. The hydrochloride salt form is expected to have similar safety considerations.
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| References | |
| Additional Infomation |
Aminoacetone hydrochloride is a research tool for studying SSAO activity and metabolism. It is a simple and stable substrate that is widely used in enzymatic assays. Its role as an endogenous metabolite links it to various metabolic pathways and diseases. It is not a drug and is not approved for therapeutic use. It is often used in combination with SSAO inhibitors to study the enzyme's function.
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| Molecular Formula |
C3H8CLNO
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|---|---|
| Molecular Weight |
109.55
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| Exact Mass |
109.029
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| CAS # |
7737-17-9
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| Related CAS # |
298-08-8 (Parent)
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| PubChem CID |
3084025
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| Appearance |
Off-white to yellow solid powder
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| Density |
0.926g/cm3
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| Boiling Point |
120.6ºC at 760mmHg
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| Flash Point |
26.8ºC
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| LogP |
1.036
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| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
6
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| Complexity |
42.2
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CC(=O)CN.Cl
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| InChi Key |
RUCLDQBBIJKQHO-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C3H7NO.ClH/c1-3(5)2-4;/h2,4H2,1H3;1H
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| Chemical Name |
1-aminopropan-2-one;hydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 9.1283 mL | 45.6413 mL | 91.2825 mL | |
| 5 mM | 1.8257 mL | 9.1283 mL | 18.2565 mL | |
| 10 mM | 0.9128 mL | 4.5641 mL | 9.1283 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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