| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| Other Sizes |
| Targets |
Stearoyl-L-carnitine-d3 chloride is used as an internal standard for the quantification of stearoyl-L-carnitine. It does not have a distinct pharmacological target separate from the unlabeled compound.
|
|---|---|
| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
As a deuterated internal standard, Stearoyl-L-carnitine-d3 chloride does not exhibit pharmacological activity distinct from that of unlabeled stearoyl-L-carnitine. The deuterium labeling does not significantly alter the biological activity of the compound. Its primary use is in analytical chemistry for the quantification of stearoyl-L-carnitine in biological samples. |
| ln Vivo |
Stearoyl-L-carnitine-d3 chloride is not used as a pharmacologically active compound in vivo. It is used as an internal standard in pharmacokinetic and metabolomic studies to accurately measure the concentration of stearoyl-L-carnitine in biological samples.
|
| Enzyme Assay |
In vitro assays for Stearoyl-L-carnitine-d3 chloride typically involve its use as an internal standard in analytical methods such as GC-MS or LC-MS. The compound is added to samples prior to extraction and analysis, and the ratio of the labeled to unlabeled compound is used to calculate the concentration of stearoyl-L-carnitine.
|
| Cell Assay |
In vitro cell-based assays using Stearoyl-L-carnitine-d3 chloride typically involve the incubation of cultured cells with the labeled compound to study fatty acid metabolism. Cells are cultured in media containing the labeled stearoyl-L-carnitine, and after a designated incubation period, metabolites are extracted and analyzed by mass spectrometry to determine the incorporation of the deuterium label into various metabolites.
|
| Animal Protocol |
In vivo animal studies with Stearoyl-L-carnitine-d3 chloride typically involve the administration of the labeled compound to rodents or other model organisms via oral gavage or intravenous injection. Following administration, blood, tissues, and urine are collected at various time points, and the samples are analyzed by mass spectrometry to trace the metabolic fate of the labeled stearoyl-L-carnitine.
|
| ADME/Pharmacokinetics |
Stearoyl-L-carnitine-d3 chloride is used as an internal standard for the quantification of stearoyl-L-carnitine in biological matrices using GC-MS or LC-MS. As a deuterated compound, it exhibits practically identical chemical properties to unlabeled stearoyl-L-carnitine, making it an ideal internal standard. Its use improves the accuracy and precision of analytical methods.
|
| Toxicity/Toxicokinetics |
No specific toxicity data are publicly available for Stearoyl-L-carnitine-d3 chloride. As a stable isotope-labeled form of an endogenous metabolite, the compound is expected to have a low toxicity profile similar to that of unlabeled stearoyl-L-carnitine.
|
| References |
|
| Additional Infomation |
Stearoyl-L-carnitine-d3 chloride is a deuterium-labeled form of stearoyl-L-carnitine intended for use as an internal standard for the quantification of stearoyl-L-carnitine by GC-MS or LC-MS. It is crucial for studies involving fatty acid metabolism, mitochondrial function, and metabolic pathway analysis. The compound is not a drug and is strictly for research purposes.
|
| Molecular Formula |
C25H47D3CLNO4
|
|---|---|
| Molecular Weight |
467.14
|
| Exact Mass |
466.361
|
| CAS # |
2245711-27-5
|
| Related CAS # |
Stearoyl-L-carnitine chloride;32350-57-5
|
| PubChem CID |
137700548
|
| Appearance |
White to light yellow solid powder
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
5
|
| Rotatable Bond Count |
22
|
| Heavy Atom Count |
31
|
| Complexity |
431
|
| Defined Atom Stereocenter Count |
1
|
| SMILES |
[2H]C([2H])([2H])[N+](C)(C)C[C@@H](CC(=O)O)OC(=O)CCCCCCCCCCCCCCCCC.[Cl-]
|
| InChi Key |
YSVYWVPJJXVIFM-UORIVOMASA-N
|
| InChi Code |
InChI=1S/C25H49NO4.ClH/c1-5-6-7-8-9-10-11-12-13-14-15-16-17-18-19-20-25(29)30-23(21-24(27)28)22-26(2,3)4;/h23H,5-22H2,1-4H3;1H/t23-;/m1./s1/i2D3;
|
| Chemical Name |
[(2R)-3-carboxy-2-octadecanoyloxypropyl]-dimethyl-(trideuteriomethyl)azanium;chloride
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1407 mL | 10.7034 mL | 21.4069 mL | |
| 5 mM | 0.4281 mL | 2.1407 mL | 4.2814 mL | |
| 10 mM | 0.2141 mL | 1.0703 mL | 2.1407 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
