| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| Other Sizes |
| Targets |
Not applicable. L-Leucine-1-13C,15N is not used to measure pharmacological activity. It is employed as an internal standard and stable isotope tracer. Its purpose is to facilitate the precise quantification of unlabeled L-Leucine or as a probe for NMR studies of protein structure and binding.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
Not applicable. L-Leucine-1-13C,15N itself is not used for measuring biological activity. It is an analytical standard and tracer. However, the parent compound L-Leucine is a potent activator of the mTOR signaling pathway. The labeled form is used to study the metabolism of leucine itself. |
| ln Vivo |
Not applicable. L-Leucine-1-13C,15N is an analytical standard and tracer. In vivo, it can be used to study protein synthesis and amino acid metabolism. A tracer dose (e.g., 1-10 mg/kg) is administered, and the enrichment of the label in plasma and tissue samples is tracked by mass spectrometry.
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| Enzyme Assay |
L-Leucine-1-13C,15N is used as an internal standard for accurate quantification of unlabeled L-Leucine. A stock solution (1 mg/mL) is prepared in 0.1 M HCl. A calibration curve is generated by spiking blank biological matrix (e.g., plasma) with known concentrations of L-Leucine (0.1-1000 ng/mL) and a fixed amount of L-Leucine-1-13C,15N (e.g., 50 ng/mL). Proteins are precipitated with acetonitrile containing the internal standard. After centrifugation, the supernatant is analyzed by LC-MS/MS in MRM mode. The mass transitions are: L-Leucine (m/z 132 → 86) and L-Leucine-1-13C,15N (m/z 134 → 87). The peak area ratio (analyte/IS) is plotted to generate a calibration curve. This method is used for absolute quantitation of leucine in plasma, urine, and tissues.
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| Cell Assay |
For cellular studies, L-Leucine-1-13C,15N is used as a tracer to measure metabolic flux through the mTOR pathway or protein synthesis. Cells are cultured in leucine-free medium supplemented with 10-100 uM of the labeled leucine for 0.5-24 hours. The label is incorporated into newly synthesized proteins. Cells are lysed, proteins are hydrolyzed, and the isotopic enrichment of the amino acid pool is measured by LC-MS/MS. This allows for the calculation of protein synthesis rates. For NMR studies, cells can be grown in medium containing this labeled compound for isotopic labeling of proteins, which is then used for structural studies of protein dynamics.
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| Animal Protocol |
L-Leucine-1-13C,15N is a high-purity, stable isotope tracer used for metabolic studies. Animals (e.g., mice or rats) are administered a tracer dose (e.g., 10 mg/kg) of the labeled leucine via oral gavage or intravenous injection. Blood samples are collected at various time points. The enrichment of the label in plasma free leucine and its metabolites is measured by GC-MS or LC-MS. These data can be used for kinetic modeling of leucine metabolism, including its rate of appearance (Ra) and oxidation. To measure protein synthesis in a specific tissue, such as muscle, a flooding dose of the tracer is administered, and the tissue is harvested after 15-60 minutes. The incorporation of the label into tissue proteins is measured by acid hydrolysis followed by mass spectrometry.
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| ADME/Pharmacokinetics |
L-Leucine-1-13C,15N (MW 133.16) is a high-purity, stable isotope-labeled compound (98 atom % ¹3C, 98 atom % ¹⁵N). It has a mass shift of +2 Da from the natural isotopic abundance form of leucine. Its physicochemical properties are identical to unlabeled L-Leucine. It is water-soluble and stable. Its specific activity is not relevant as it is a stable isotope.
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| Toxicity/Toxicokinetics |
L-Leucine-1-13C,15N is a non‑toxic, stable isotope‑labeled compound. At the concentrations used as an internal standard (ng/mL levels) or as a tracer (mg/kg levels), it poses no toxicological risk. The unlabeled parent compound, L-Leucine, is an essential amino acid safe for consumption.
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| References | |
| Additional Infomation |
L-Leucine-1-13C,15N is a stable double isotope-labeled internal standard for accurate quantitation of L-Leucine by LC-MS. CAS: 80134-83-4.
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| Molecular Formula |
C513CH1315NO2
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|---|---|
| Molecular Weight |
133.16
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| Exact Mass |
133.095
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| CAS # |
80134-83-4
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| Related CAS # |
L-Leucine;61-90-5
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| PubChem CID |
16213457
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| Appearance |
White to off-white solid powder
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| Density |
1.035 g/cm3
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| Melting Point |
>300ºC(lit.)
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| LogP |
1.144
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
9
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| Complexity |
101
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| Defined Atom Stereocenter Count |
1
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| SMILES |
CC(C)C[C@@H]([13C](=O)O)[15NH2]
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| InChi Key |
ROHFNLRQFUQHCH-XAAHKOKXSA-N
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| InChi Code |
InChI=1S/C6H13NO2/c1-4(2)3-5(7)6(8)9/h4-5H,3,7H2,1-2H3,(H,8,9)/t5-/m0/s1/i6+1,7+1
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| Chemical Name |
(2S)-2-(15N)azanyl-4-methyl(113C)pentanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 7.5098 mL | 37.5488 mL | 75.0976 mL | |
| 5 mM | 1.5020 mL | 7.5098 mL | 15.0195 mL | |
| 10 mM | 0.7510 mL | 3.7549 mL | 7.5098 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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