| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
No specific primary biological target has been identified for Acid Black 2 (Nigrosine), as it functions primarily as a dye and staining reagent rather than a direct pharmacological agent. However, the compound interacts with biological molecules through ionic and hydrophobic interactions. As an acid dye, Acid Black 2 binds to positively charged groups on proteins and other biological macromolecules, enabling its use as a stain for nucleic acids and proteins. The dye's sulfonate groups provide anionic character that facilitates binding to cationic sites on biological structures. In histological applications, the compound stains various tissue components, allowing for visualization of cellular and subcellular structures under the microscope. The compound's ability to bind to specific cellular components is based on its chemical structure rather than a specific receptor-ligand interaction.
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| ln Vitro |
In vitro, Acid Black 2 is extensively used as a biological stain for the detection of nucleic acids and proteins in electrophoresis and microscopy applications. It is also used in histological staining procedures for the visualization of various tissue components. In biochemical research, the compound serves as a reagent for studying protein-nucleic acid interactions and for visualizing biomolecules in gel electrophoresis. The dye's unique chemical properties allow it to bind to specific cellular components, producing color changes that enable visualization. In the textile industry, it is used as a dye for wool, silk, and nylon. The compound is also employed in the development of colorimetric assays for the detection of various analytes. Its ability to form complexes with biomolecules makes it useful for studying protein structure and function.
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| ln Vivo |
In vivo studies are not typically performed with Acid Black 2 (Nigrosine), as it is primarily a staining reagent and dye rather than a pharmacological agent. The compound is not intended for administration to living organisms for therapeutic purposes. However, the dye may be used in vivo for certain experimental applications such as vital staining or tracking of biological processes. In toxicological studies, the compound's potential for systemic absorption and toxicity may be evaluated, but comprehensive in vivo studies on the parent compound are not documented. The compound is primarily used in vitro for research and industrial applications rather than for in vivo experimentation.
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| Enzyme Assay |
Cell-free assays involving Acid Black 2 are focused on its use as a staining reagent. Standard protocols for protein staining in electrophoresis involve preparing a staining solution of Acid Black 2 in methanol, acetic acid, and water. Gels are incubated in the staining solution for a specified time, followed by destaining in a methanol-acetic acid-water solution to remove excess dye. For microscopy applications, tissue sections are incubated with the dye solution, washed, and examined under the microscope. The dye's binding to nucleic acids and proteins can be quantified spectrophotometrically by measuring absorbance at the dye's characteristic wavelength. The compound's interactions with biomolecules can be studied using various techniques including UV-Vis spectroscopy and fluorescence microscopy.
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| Cell Assay |
Cellular assays with Acid Black 2 involve its use as a staining reagent for the visualization of cellular components. In histological staining, cells or tissue sections are incubated with the dye solution, washed, and examined under the microscope. The dye binds to specific cellular components such as nucleic acids and proteins, producing color changes that enable visualization of cellular structures. The compound can also be used in flow cytometry for cell staining applications. In cell viability assays, the dye may be used to assess membrane integrity, as viable cells exclude the dye while dead cells take it up. The dye's staining properties can be optimized by adjusting concentration, pH, and incubation time to achieve specific staining patterns.
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| Animal Protocol |
Animal studies are not typically conducted with Acid Black 2 (Nigrosine), as it is primarily a staining reagent and dye rather than a pharmacological agent. The compound is not intended for administration to animals for therapeutic purposes. However, the dye may be used in certain experimental applications such as vital staining or for tracking biological processes in animal models. In toxicological studies, the compound's potential for systemic absorption, tissue distribution, and toxicity may be evaluated in animal models. The compound's use as a dye means that comprehensive in vivo studies are not typically performed, and its safety profile is primarily based on its use as a research reagent and industrial dye.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for Acid Black 2 (Nigrosine) are not available, as the compound is primarily a dye and staining reagent rather than a drug candidate. With a molecular weight of 616.49 g/mol, the compound is a large molecule that would be expected to have limited membrane permeability and oral bioavailability if administered. The compound is water-soluble, which may facilitate renal excretion if absorbed. However, comprehensive pharmacokinetic studies including absorption, distribution, metabolism, and excretion have not been performed, as the compound is not intended for therapeutic use. For research purposes, the compound is typically handled as a neat chemical and not administered to living organisms for pharmacokinetic profiling. The compound's large size and ionic nature suggest limited systemic absorption following exposure.
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| Toxicity/Toxicokinetics |
Toxicological data for Acid Black 2 (Nigrosine) are limited. The compound is a synthetic dye that is primarily used as a staining reagent and industrial dye. As with many synthetic dyes, the compound may cause skin and eye irritation upon contact. Appropriate safety precautions should be taken when handling the compound, including the use of personal protective equipment such as gloves and safety goggles. The compound should be handled in a well-ventilated area, and contact with skin and eyes should be avoided. In case of exposure, affected areas should be rinsed thoroughly with water. The compound is not classified as a carcinogen or mutagen based on available data, but comprehensive toxicological evaluation has not been performed. The compound requires protection from light during transportation and storage.
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| Additional Infomation |
Ci Acid Black 1 is a dark brown granular solid. (NTP, 1992) Amide Black 10B is an organosodium salt formed by the condensation of Amide Black 10B (acidic form) with two equivalents of sodium hydroxide. It is used as a histological dye. Its structure contains 4-amino-5-hydroxy-3-[(p-nitrophenyl)azo]-6-(phenylazo)-naphthalene-2,7-disulfonate.
Acid Black 2 (Nigrosine) is a research chemical and dye, not an approved drug. It has no clinical trial or marketing approval status for therapeutic use. The compound is widely used as a biological stain for the detection of nucleic acids and proteins in electrophoresis and microscopy applications. It is also used in the textile industry as a dye for wool, silk, and nylon. As a biochemical compound, it can be utilized as a biomaterial or organic/chemical reagent for biomedical research. The compound has a molecular formula of C22H14N6Na2O9S2 and a molecular weight of 616.49 g/mol. It should be stored at -20°C for long-term stability and requires protection from light during transportation and storage. The compound is for research use only and not for human use. |
| Molecular Formula |
C22H14N6NA2O9S2
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| Molecular Weight |
616.4909
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| Exact Mass |
616.005
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| CAS # |
8005-03-6
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| Related CAS # |
3121-74-2 (parent cpd);83006-55-7 (hydrochloride salt);85294-31-1 (di-lithium salt);1064-48-8 (Parent)
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| PubChem CID |
135442942
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| Appearance |
Dark purple to black solid powder
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| Melting Point |
greater than 662 °F (NTP, 1992)
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| LogP |
7.94
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
14
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
41
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| Complexity |
1110
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| Defined Atom Stereocenter Count |
0
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O: 2.86 mg/mL
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.6221 mL | 8.1104 mL | 16.2209 mL | |
| 5 mM | 0.3244 mL | 1.6221 mL | 3.2442 mL | |
| 10 mM | 0.1622 mL | 0.8110 mL | 1.6221 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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