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|---|---|---|
| Other Sizes |
| Targets |
Amido Black 10B does not have a specific biological target. Its mechanism of action is based on its chemical properties as a diazo dye, which allows it to bind non-specifically to proteins through electrostatic and hydrophobic interactions. This property enables its use as a general protein stain in various electrophoretic and blotting applications.
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| ln Vitro |
In vitro, Amido Black 10B is used to stain proteins for visualization and quantification. It binds to proteins in gels and on membranes, allowing for the detection of protein bands. It is also utilized in cell proliferation assays and histochemical studies. The dye's staining intensity is proportional to the amount of protein present, making it useful for semi-quantitative analysis.
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| ln Vivo |
In vivo applications of Amido Black 10B are not typical, as it is primarily a research reagent used in vitro. It is not administered to living organisms for therapeutic or diagnostic purposes. Its use is confined to laboratory settings for protein analysis.
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| Enzyme Assay |
Cell-free assays for Amido Black 10B are not applicable as it is not an enzyme inhibitor. Its properties as a dye are characterized by its binding affinity to proteins in solution or on membranes. The compound's staining efficiency can be assessed spectrophotometrically by measuring the absorbance of the dye-protein complex.
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| Cell Assay |
Cellular experiments with Amido Black 10B are not standard, as it is primarily used for staining proteins in gels and membranes. It can be used in cell proliferation assays, but its main application is in protein biochemistry.
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| Animal Protocol |
In vivo animal experiments are not applicable for Amido Black 10B as it is a staining reagent used ex vivo, not a therapeutic agent.
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| ADME/Pharmacokinetics |
Amido Black 10B is a dye and does not have conventional pharmacokinetic properties. It is a solid with a molecular weight of 616.49 g/mol. For storage, it should be kept in a cool, dry place, protected from light. It is soluble in water at approximately 30 g/L at 20°C.
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| Toxicity/Toxicokinetics |
Amido Black 10B is a highly toxic azo dye. It can cause respiratory problems in humans and may also cause skin and eye irritation. It is not intended for human consumption or therapeutic use. Standard safety precautions, including the use of gloves and eye protection, are essential when handling this compound.
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| References | |
| Additional Infomation |
Ci Acid Black 1 is a dark brown granular solid. (NTP, 1992) Amide Black 10B is an organosodium salt formed by the condensation of Amide Black 10B (acidic form) with two equivalents of sodium hydroxide. It is used as a histological dye. Its structure contains 4-amino-5-hydroxy-3-[(p-nitrophenyl)azo]-6-(phenylazo)-naphthalene-2,7-disulfonate.
Amido Black 10B (CAS: 1064-48-8) is a diazo dye also known by synonyms including Naphthol Blue Black, Acid Black 1, and C.I. 20470. It is a histological, protein, and nucleic acid dye. In addition to its use in biochemical research, it is also employed in criminal investigations to detect blood in latent fingerprints. |
| Molecular Formula |
C22H14N6NA2O9S2
|
|---|---|
| Molecular Weight |
616.4909
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| Exact Mass |
616.005
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| CAS # |
1064-48-8
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| Related CAS # |
3121-74-2 (parent cpd);83006-55-7 (hydrochloride salt);85294-31-1 (di-lithium salt);1064-48-8 (Parent)
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| PubChem CID |
135442942
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| Appearance |
Brown to black solid powder
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| Density |
1.05 g/mL at 20 °C
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| Melting Point |
greater than 662 °F (NTP, 1992)
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| Flash Point |
30 °C
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| LogP |
7.94
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
14
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
41
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| Complexity |
1110
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C1=CC=C(C=C1)N=NC2=C(C3=C(C(=C(C=C3C=C2S(=O)(=O)[O-])S(=O)(=O)[O-])N=NC4=CC=C(C=C4)[N+](=O)[O-])N)O.[Na+].[Na+]
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| InChi Key |
AOMZHDJXSYHPKS-UHFFFAOYSA-L
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| InChi Code |
InChI=1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2
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| Chemical Name |
disodium;4-amino-5-hydroxy-3-[(4-nitrophenyl)diazenyl]-6-phenyldiazenylnaphthalene-2,7-disulfonate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.6221 mL | 8.1104 mL | 16.2209 mL | |
| 5 mM | 0.3244 mL | 1.6221 mL | 3.2442 mL | |
| 10 mM | 0.1622 mL | 0.8110 mL | 1.6221 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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