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| 5mg |
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| Other Sizes |
| Targets |
Medroxyprogesterone-d3 targets the progesterone receptor through its non-deuterated parent compound, Medroxyprogesterone, which is a potent progesterone receptor agonist. By binding to and activating the progesterone receptor, medroxyprogesterone modulates the transcription of progesterone-responsive genes involved in reproductive function, endometrial maturation, and hormonal regulation. The compound has been used in hormone replacement therapy and as a component of oral contraceptives. Its deuterated form serves as an internal standard for analytical quantification.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
As a deuterated internal standard, Medroxyprogesterone-d3 does not have intrinsic in vitro biological activity that is separately characterized from its non-deuterated parent compound. Medroxyprogesterone, the non-labeled form, is a synthetic progestin and a potent progesterone receptor agonist. In vitro, it binds to and activates progesterone receptors, modulating the transcription of progesterone-responsive genes. Its activity is typically assessed in cell-based reporter assays using progesterone response element (PRE)-luciferase constructs. The deuterated form serves as an internal standard in these studies. |
| ln Vivo |
Medroxyprogesterone-d3 is not used for in vivo pharmacological activity assessment as a standalone compound. Its primary application is as an internal standard for the quantification of medroxyprogesterone in biological samples. Medroxyprogesterone, the non-deuterated form, is an orally active progestogen used in hormone replacement therapy and as a component of oral contraceptives. It is also used in veterinary practice as an estrus regulator. The deuterated internal standard enables accurate measurement of medroxyprogesterone levels in pharmacokinetic and pharmacodynamic studies.
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| Enzyme Assay |
In vitro enzyme/receptor binding assays for Medroxyprogesterone-d3 typically involve its use as an internal standard in competitive binding assays rather than as a test compound itself. The non-deuterated medroxyprogesterone can be assessed for binding affinity to progesterone receptor using radioligand binding assays with appropriate tracers. Assays are conducted in buffered solutions at physiological pH with appropriate receptor preparations. Binding affinity is expressed as IC₅₀ or Kd values. The deuterated compound serves as a quality control standard.
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| Cell Assay |
In vitro cell-based assays using Medroxyprogesterone-d3 typically employ the compound as an internal standard for LC-MS/MS quantification in cell culture media or cell lysates rather than as a test compound. For studying medroxyprogesterone's biological activity, cell lines expressing progesterone receptor are used to assess receptor activation through reporter gene assays with PRE-luciferase constructs. Gene expression analysis by qPCR can also be employed. Standard cell culture conditions (37°C, 5% CO₂) with appropriate media are used.
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| Animal Protocol |
In vivo animal studies utilizing Medroxyprogesterone-d3 typically involve administration of the deuterated compound as a tracer or internal standard for pharmacokinetic or pharmacodynamic studies. The compound is used to quantify endogenous or exogenous medroxyprogesterone levels in plasma, urine, and tissue samples from animal models of reproductive function or hormone therapy. Typical study designs involve sample collection at various time points, followed by LC-MS/MS analysis using Medroxyprogesterone-d3 as the internal standard. All procedures comply with institutional animal care and use guidelines.
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| ADME/Pharmacokinetics |
Medroxyprogesterone-d3 has a molecular weight of 347.51 g/mol and a molecular formula of C₂₂H₂₉D₃O₃. It is used as an internal standard for the quantification of medroxyprogesterone by LC-mass spectrometry. As an isotope-labeled compound, its chromatographic and mass spectrometric behavior is nearly identical to that of non-deuterated medroxyprogesterone, allowing for precise correction of analytical variability. The compound is typically stored under conditions recommended for stable isotope-labeled standards.
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| Toxicity/Toxicokinetics |
Medroxyprogesterone-d3 is intended for research and analytical use only and is not approved for human therapeutic applications. As a stable isotope-labeled internal standard, it is used in trace quantities in analytical methods and does not present significant toxicological concerns at these levels. The non-deuterated parent compound, medroxyprogesterone, is a synthetic progestin with well-characterized safety and efficacy profiles. Standard laboratory safety precautions should be observed when handling Medroxyprogesterone-d3.
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| References | |
| Additional Infomation |
Medroxyprogesterone-d3 (CAS#: 162462-69-3) has a molecular formula of C₂₂H₂₉D₃O₃ and a molecular weight of 347.51 g/mol. It is the deuterium-labeled form of Medroxyprogesterone (CAS#: 520-85-4). Medroxyprogesterone is a synthetic progestin and potent progesterone receptor agonist. It is used in hormone replacement therapy and has been used in oral contraceptives. Medroxyprogesterone-d3 is intended for use as an internal standard for GC- or LC-MS quantification. This compound is not a drug and is strictly a research reagent.
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| Molecular Formula |
C22H29D3O3
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| Molecular Weight |
347.50616
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| Exact Mass |
347.253
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| CAS # |
162462-69-3
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| Related CAS # |
Medroxyprogesterone;520-85-4
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| PubChem CID |
46782109
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| Appearance |
Off-white to gray solid powder
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| Melting Point |
204-206°C
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| LogP |
3.5
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
25
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| Complexity |
664
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| Defined Atom Stereocenter Count |
7
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| SMILES |
CC1CC2C(CCC3(C2CCC3(C(=O)C)O)C)C4(C1=CC(=O)CC4)C
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| InChi Key |
FRQMUZJSZHZSGN-ZLUPVJNASA-N
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| InChi Code |
InChI=1S/C22H32O3/c1-13-11-16-17(20(3)8-5-15(24)12-19(13)20)6-9-21(4)18(16)7-10-22(21,25)14(2)23/h12-13,16-18,25H,5-11H2,1-4H3/t13-,16+,17-,18-,20+,21-,22-/m0/s1/i1D3
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| Chemical Name |
(6S,8R,9S,10R,13S,14S,17R)-17-acetyl-17-hydroxy-10,13-dimethyl-6-(trideuteriomethyl)-2,6,7,8,9,11,12,14,15,16-decahydro-1H-cyclopenta[a]phenanthren-3-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.8776 mL | 14.3881 mL | 28.7762 mL | |
| 5 mM | 0.5755 mL | 2.8776 mL | 5.7552 mL | |
| 10 mM | 0.2878 mL | 1.4388 mL | 2.8776 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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