| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg | |||
| Other Sizes |
| Targets |
IC50: 140 μM (monoamine oxidase)[1]
Glicoricone targets two primary pathways: the central nervous system via monoamine oxidase (MAO) and the endocrine system via the Estrogen Receptor (ER). It binds to ER and acts as an antagonist, while also inhibiting the activity of MAO with an IC50 of 140 microM. |
|---|---|
| ln Vitro |
Glicoricone is an inhibitor of monoamine oxidase (MAO), with an IC50 of 140 microM. It binds to the estrogen receptor (ER) and shows estrogen antagonist activity. These activities make it a unique tool for studying the complex pharmacology of licorice-derived compounds.
|
| ln Vivo |
As a dual MAO inhibitor and ER antagonist, Glicoricone offers a multi-targeted approach for in vivo research. It is being studied for its potential effects on mood and anxiety (via MAO inhibition) and on hormone-sensitive tissues (via ER antagonism). Its pharmacological profile contributes to the diverse effects of licorice root extract.
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| Enzyme Assay |
Cell-free MAO inhibition assays are performed using purified MAO-A or MAO-B enzymes. The assay uses kynuramine as a substrate; MAO converts it to 4-hydroxyquinoline, which is a fluorescent product. Glicoricone is pre-incubated with the enzyme, and the reaction is started by adding the substrate. After 60 minutes at 37degC, the increase in fluorescence (excitation 310 nm, emission 400 nm) is measured to determine the IC50.
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| Cell Assay |
Cell-based ER antagonist assays are performed using T47D or MCF-7 breast cancer cells stably transfected with an estrogen response element (ERE)-luciferase reporter. Cells are treated with 1 nM 17beta-estradiol (E2) and varying concentrations of Glicoricone for 24 hours. The cells are then lysed, and luciferase activity is measured. The IC50 is determined by the compound's ability to block E2-induced luciferase expression.
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| Animal Protocol |
In vivo data for Glicoricone is limited as a pure compound, as it is often studied as part of licorice extract. Animal models for estrogen receptor antagonism (e.g., ovariectomized rat uterotrophic assay) and MAO inhibition (e.g., mouse forced swim test) are used. These studies help determine its potency and bioavailability relative to standard drugs.
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| ADME/Pharmacokinetics |
The pharmacokinetic profile of Glicoricone is a subject of ongoing research. As a natural product, it is expected to have moderate oral bioavailability following extraction from licorice. Understanding its metabolism and distribution is key to interpreting its multi-faceted effects in vivo.
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| Toxicity/Toxicokinetics |
Glicoricone is considered a relatively safe phytochemical, as it is present in licorice consumed by humans. However, at high doses, its ER antagonist and MAO inhibitory activities could lead to hormonal changes or drug-drug interactions. Standard safety precautions for handling natural products apply.
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| References |
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| Additional Infomation |
Glicoricone is one of the isoflavone compounds. It has been reported that Glicoricone exists in plants of the genus Glycyrrhiza (uralensis) and Glycyrrhiza (with relevant data).
Glicoricone is a unique natural product tool that provides insight into the "multi-targeted" pharmacology of botanical extracts. It is used to study the role of licorice derivatives in complementary medicine and to develop novel pharmaceuticals for complex diseases like depression and hormone-related cancers. It is intended for research use only. |
| Molecular Formula |
C21H20O6
|
|---|---|
| Molecular Weight |
368.3799
|
| Exact Mass |
368.125
|
| CAS # |
161099-37-2
|
| PubChem CID |
10361658
|
| Appearance |
White to yellow solid powder
|
| Melting Point |
192 °C
|
| LogP |
4
|
| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
6
|
| Rotatable Bond Count |
4
|
| Heavy Atom Count |
27
|
| Complexity |
608
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
O(C([H])([H])[H])C1=C(C(=C([H])C(=C1C1=C([H])OC2C([H])=C(C([H])=C([H])C=2C1=O)O[H])O[H])O[H])C([H])([H])/C(/[H])=C(\C([H])([H])[H])/C([H])([H])[H]
|
| InChi Key |
SSDIPYMSXRNGMZ-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C21H20O6/c1-11(2)4-6-13-16(23)9-17(24)19(21(13)26-3)15-10-27-18-8-12(22)5-7-14(18)20(15)25/h4-5,7-10,22-24H,6H2,1-3H3
|
| Chemical Name |
3-[4,6-dihydroxy-2-methoxy-3-(3-methylbut-2-enyl)phenyl]-7-hydroxychromen-4-one
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7146 mL | 13.5729 mL | 27.1459 mL | |
| 5 mM | 0.5429 mL | 2.7146 mL | 5.4292 mL | |
| 10 mM | 0.2715 mL | 1.3573 mL | 2.7146 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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