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| 10mg |
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| Targets |
As an isotope-labeled internal standard, Cholesterol-d7 does not have a defined biological target as a standalone compound. Its primary application is as an analytical standard for the quantification of the endogenous sterol cholesterol. Cholesterol, the non-deuterated parent compound, is the major sterol in mammals, making up 20-25% of the structural component of the plasma membrane and serving as a precursor for steroid hormones, bile acids, and vitamin D. The deuterated form serves as an internal standard for accurate measurement in analytical studies.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
As a deuterated internal standard, Cholesterol-d7 does not have intrinsic in vitro biological activity that is separately characterized from its non-deuterated parent compound. Cholesterol, the non-labeled form, is the major sterol in mammals and is essential for membrane integrity and function. In vitro, its effects on membrane fluidity, cell signaling, and lipid metabolism are well studied. The deuterated form serves as an internal standard in analytical studies rather than as a test compound for biological activity assessment. |
| ln Vivo |
Cholesterol-d7 is not used for in vivo pharmacological activity assessment as a standalone compound. Its primary application is as an internal standard for the quantification of cholesterol in biological samples from in vivo studies. Cholesterol, the non-deuterated form, is the major sterol in mammals and plays a critical role in membrane structure, steroid hormone biosynthesis, and bile acid production. The deuterated internal standard enables accurate measurement of cholesterol levels in metabolic and cardiovascular studies.
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| Enzyme Assay |
In vitro assays for Cholesterol-d7 typically involve analytical methods for its detection and quantification rather than biological activity assessment. The compound is used as an internal standard in GC-MS or LC-MS methods for the quantification of cholesterol in plasma, serum, tissues, and other biological matrices. Sample preparation typically involves lipid extraction, saponification, and derivatization for GC-MS analysis, or direct injection for LC-MS analysis. The compound serves as a critical quality control standard in these analytical methods.
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| Cell Assay |
Cholesterol-d7 is not used in cell-based assays as a test compound. Its primary application is as an internal standard for LC-MS or GC-MS quantification in cell culture media or cell lysates from in vitro studies. The compound is used to correct for analytical variability in the quantification of cholesterol in samples from cell-based lipid metabolism, cholesterol efflux, and sterol biosynthesis studies. Standard analytical procedures are employed for its characterization and quantification.
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| Animal Protocol |
Cholesterol-d7 is not used in animal studies as a pharmacological agent. Its primary application is as an internal standard for the quantification of cholesterol in plasma, serum, and tissue samples from metabolic and cardiovascular studies. The compound is typically administered as a tracer or used as a spiked internal standard in sample analysis. Sample collection at various time points is followed by GC-MS or LC-MS analysis using Cholesterol-d7 as the internal standard. All procedures comply with institutional animal care and use guidelines.
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| ADME/Pharmacokinetics |
Cholesterol-d7 is used as an internal standard for the quantification of cholesterol by GC- or LC-mass spectrometry. It has a molecular weight of 393.70 g/mol and a molecular formula of C₂₇H₃₉D₇O. As an isotope-labeled compound, its chromatographic and mass spectrometric behavior is nearly identical to that of non-deuterated cholesterol, allowing for precise correction of analytical variability. The compound is typically stored at 2-8°C refrigerated or under conditions recommended for stable isotope-labeled standards. Its pharmacokinetic properties as a tracer mirror those of endogenous cholesterol.
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| Toxicity/Toxicokinetics |
Cholesterol-d7 is intended for research and analytical use only and is not approved for human therapeutic applications. As a stable isotope-labeled internal standard, it is used in trace quantities in analytical methods and does not present significant toxicological concerns at these levels. The non-deuterated parent compound, cholesterol, is an endogenous sterol with well-characterized physiological roles. Standard laboratory safety precautions should be observed when handling Cholesterol-d7, including the use of appropriate personal protective equipment.
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| References | |
| Additional Infomation |
Cholesterol_d7 is a type of cholesterol.
Cholesterol-d7 (cholesterol d7) (CAS#: 83199-47-7) has a molecular formula of C₂₇H₃₉D₇O and a molecular weight of 393.70 g/mol. It is the deuterium-labeled form of Cholesterol. Cholesterol is the major sterol in mammals, making up 20-25% of plasma membrane structural components. Cholesterol-d7 is intended for use as an internal standard for GC- or LC-MS quantification of cholesterol. This compound is not a drug and is strictly a research reagent for analytical applications. |
| Molecular Formula |
C27H39D7O
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|---|---|
| Molecular Weight |
393.70
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| Exact Mass |
393.399
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| CAS # |
83199-47-7
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| Related CAS # |
Cholesterol;57-88-5;Cholesterol-d6;60816-17-3
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| PubChem CID |
5314029
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| Appearance |
White to off-white solid powder
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| Density |
1g/cm3
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| Boiling Point |
480.6ºC at 760 mmHg
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| Melting Point |
141-143ºC
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| Flash Point |
209.3ºC
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| Index of Refraction |
1.525
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| LogP |
7.388
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
1
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
28
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| Complexity |
591
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| Defined Atom Stereocenter Count |
8
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| SMILES |
[2H]C([2H])([2H])C([2H])(CCC[C@@H](C)[C@H]1CC[C@@H]2[C@@]1(CC[C@H]3[C@H]2CC=C4[C@@]3(CC[C@@H](C4)O)C)C)C([2H])([2H])[2H]
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| InChi Key |
HVYWMOMLDIMFJA-IFAPJKRJSA-N
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| InChi Code |
InChI=1S/C27H46O/c1-18(2)7-6-8-19(3)23-11-12-24-22-10-9-20-17-21(28)13-15-26(20,4)25(22)14-16-27(23,24)5/h9,18-19,21-25,28H,6-8,10-17H2,1-5H3/t19-,21+,22+,23-,24+,25+,26+,27-/m1/s1/i1D3,2D3,18D
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| Chemical Name |
(3S,8S,9S,10R,13R,14S,17R)-10,13-dimethyl-17-[(2R)-6,7,7,7-tetradeuterio-6-(trideuteriomethyl)heptan-2-yl]-2,3,4,7,8,9,11,12,14,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthren-3-ol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.5400 mL | 12.7000 mL | 25.4001 mL | |
| 5 mM | 0.5080 mL | 2.5400 mL | 5.0800 mL | |
| 10 mM | 0.2540 mL | 1.2700 mL | 2.5400 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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