| Size | Price | Stock | Qty |
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| 100mg |
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| 500mg |
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| Other Sizes |
| Targets |
Rhodamine 101 inner salt does not have a defined pharmacological target as it is a fluorescent dye and biochemical reagent. The compound is widely used in various biological applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy, and ELISA. Its fluorescence properties enable detection and imaging in biological research.
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| ln Vitro |
In vitro activity of Rhodamine 101 inner salt is assessed in fluorescence-based applications. The compound exhibits bright fluorescence with high photostability. It is used as a reference model for analysing extra fluorochromic properties of rhodamine derivatives. The dye is also used in various fields including medical, environmental, and industrial research.
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| ln Vivo |
No specific in vivo pharmacological activity data have been documented for Rhodamine 101 inner salt as a therapeutic agent. The compound is used as a fluorescent dye and biochemical reagent. It is not administered to animals for pharmacological evaluation. Its applications are limited to in vitro imaging and detection research.
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| Enzyme Assay |
For non-cellular assays, Rhodamine 101 inner salt is characterized by standard analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity and purity. The compound can be evaluated as a fluorescent standard in spectroscopic assays. Typical protocols involve dissolving the compound in appropriate solvents and measuring fluorescence. Purity: ≥94-95%. Excitation: 565 nm, Emission: 595 nm.
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| Cell Assay |
For in vitro cell-based studies, Rhodamine 101 inner salt is used as a fluorescent probe in various applications including fluorescence microscopy, flow cytometry, and ELISA. Cells are stained with the dye and analyzed using appropriate fluorescence detection methods. The compound is typically dissolved in ethanol at 0.1 mg/mL. Appropriate controls should be included.
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| Animal Protocol |
For in vivo animal studies, Rhodamine 101 inner salt is not typically administered as a test compound. The compound is used as a fluorescent dye for in vitro applications. If used in animal studies for imaging purposes, it would be administered via appropriate routes with suitable formulations. Storage: -20°C. Shipping: room temperature.
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| ADME/Pharmacokinetics |
Rhodamine 101 inner salt has a molecular weight of 490.60 and molecular formula C32H30N2O3. Excitation maximum: 565 nm, Emission maximum: 595 nm. Purity: ≥94-95%. Storage: -20°C in the dark. Solubility: ethanol at 0.1 mg/mL.
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| Toxicity/Toxicokinetics |
Rhodamine 101 inner salt is for research use only and not for human or veterinary use. Standard laboratory safety practices should be followed when handling this chemical. Appropriate personal protective equipment including gloves and safety glasses should be worn. Specific LD50 values and detailed toxicological profiles have not been extensively reported.
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| Additional Infomation |
Rhodamine 101 is an imine betaine and an organic heterocyclic compound. It can be used as a fluorescent dye.
Rhodamine 101 inner salt (CAS 116450-56-7) is also known as Rhodamine 640 and RH101. It is a bright fluorescent dye with excitation and emission maxima of 565 and 595 nm, respectively. Applications include fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy, and ELISA. No clinical trials or therapeutic approvals exist. |
| Molecular Formula |
C32H30N2O3
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|---|---|
| Molecular Weight |
490.59
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| Exact Mass |
490.225
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| CAS # |
116450-56-7
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| Related CAS # |
Rhodamine 101 chloride; 64339-18-0; Rhodamine 101 hydrochloride
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| PubChem CID |
2735129
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| Appearance |
Brown to reddish brown solid
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| LogP |
4.9
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
37
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| Complexity |
1070
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C1CC2=CC3=C(C4=C2N(C1)CCC4)OC5=C6CCC[N+]7=C6C(=CC5=C3C8=CC=CC=C8C(=O)[O-])CCC7
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| InChi Key |
MUSLHCJRTRQOSP-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C32H30N2O3/c35-32(36)22-10-2-1-9-21(22)27-25-17-19-7-3-13-33-15-5-11-23(28(19)33)30(25)37-31-24-12-6-16-34-14-4-8-20(29(24)34)18-26(27)31/h1-2,9-10,17-18H,3-8,11-16H2
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| Chemical Name |
2-(3-oxa-23-aza-9-azoniaheptacyclo[17.7.1.15,9.02,17.04,15.023,27.013,28]octacosa-1(27),2(17),4,9(28),13,15,18-heptaen-16-yl)benzoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.0384 mL | 10.1918 mL | 20.3836 mL | |
| 5 mM | 0.4077 mL | 2.0384 mL | 4.0767 mL | |
| 10 mM | 0.2038 mL | 1.0192 mL | 2.0384 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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