| Size | Price | Stock | Qty |
|---|---|---|---|
| 10mg |
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| 100mg | |||
| Other Sizes |
| Targets |
Rhodamine 101 chloride does not have a specific biological target in the traditional sense of a drug target. As a fluorescent dye, its primary function is to bind to cellular components and emit fluorescence upon excitation. The compound's cationic nature allows efficient binding to negatively charged cellular components, including nucleic acids, membranes, and proteins. This property enables the dye to be used for labeling and visualizing biological structures in various applications. Rhodamine 101 is also used as a reference standard for labeled bioconjugates. The dye's fluorescence properties make it a valuable tool for biological imaging and detection applications.
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|---|---|
| ln Vitro |
In vitro, Rhodamine 101 chloride is used as a fluorescent labeling reagent in various biological assays. The dye exhibits exceptional photostability and brightness, making it suitable for long-term imaging applications. Its maximum excitation and emission wavelengths of 565 nm and 595 nm, respectively, are compatible with standard fluorescence detection systems. The compound's cationic nature allows efficient binding to negatively charged cellular components, enabling precise visualization of biological structures. Rhodamine 101 chloride is used in microscopy, flow cytometry, fluorescence correlation spectroscopy, and ELISA applications. It is also used as a reference standard for 5-ROX or 6-ROX labeled bioconjugates.
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| ln Vivo |
Rhodamine 101 chloride is not typically used for in vivo applications as a therapeutic agent. However, fluorescent dyes like Rhodamine 101 are used in preclinical imaging studies for tracking cells, visualizing tissues, and monitoring biological processes. The dye's bright fluorescence and photostability make it suitable for in vivo imaging applications where long-term signal stability is required. However, the use of Rhodamine 101 chloride in vivo is limited by potential toxicity and the need for appropriate delivery methods. The compound is primarily used as a research tool rather than as a therapeutic agent.
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| Enzyme Assay |
The in vitro receptor binding or enzyme assays for Rhodamine 101 chloride are not applicable, as the compound is a fluorescent dye rather than a drug targeting specific receptors or enzymes. Instead, the compound is used as a detection reagent in various assays. In fluorescence-based assays, Rhodamine 101 chloride is used as a label for biomolecules, and its fluorescence is measured to quantify binding or enzymatic activity. The dye's excitation and emission properties are characterized using spectrophotometry. The compound's photostability and brightness are assessed under various conditions to ensure optimal performance in imaging applications.
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| Cell Assay |
Cellular assays using Rhodamine 101 chloride typically involve staining cells with the dye and visualizing them using fluorescence microscopy or flow cytometry. Cells are cultured on coverslips or in suspension, incubated with the dye at appropriate concentrations, and washed to remove unbound dye. Stained cells are then examined using a fluorescence microscope with appropriate excitation and emission filters (565 nm excitation, 595 nm emission). For flow cytometry, stained cells are analyzed to measure fluorescence intensity. The dye's cationic nature allows efficient binding to negatively charged cellular components, enabling precise visualization of cellular structures. These protocols are standard for fluorescent dye applications.
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| Animal Protocol |
Rhodamine 101 chloride is not used in typical in vivo animal studies as a therapeutic agent. However, the dye may be used in preclinical imaging studies for tracking cells or visualizing tissues in animal models. In such studies, the dye is administered locally or systemically, and its distribution and fluorescence are monitored using appropriate imaging systems. The compound's bright fluorescence and photostability make it suitable for these applications. However, the use of Rhodamine 101 chloride in vivo is limited by potential toxicity and the need for appropriate delivery methods. Detailed in vivo protocols for this compound are not well-documented.
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| ADME/Pharmacokinetics |
Rhodamine 101 chloride has a molecular weight of 527.05 and a molecular formula of C32H31ClN2O3. The compound is a solid at room temperature. As a fluorescent dye, its key properties are its excitation and emission wavelengths (565 nm and 595 nm, respectively). The dye is soluble in appropriate solvents for biological applications. Detailed pharmacokinetic parameters such as absorption, distribution, metabolism, and excretion are not relevant for a fluorescent dye used primarily as a research tool. The compound should be stored under appropriate conditions to maintain its fluorescence properties.
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| Toxicity/Toxicokinetics |
Toxicological data for Rhodamine 101 chloride are limited. As a fluorescent dye used primarily for research purposes, the compound is not intended for therapeutic use. Standard safety precautions should be followed when handling the compound, including the use of appropriate personal protective equipment. The compound's toxicity profile has not been systematically evaluated in preclinical studies. Researchers should consult the Safety Data Sheet (SDS) for specific safety information. The compound is for research use only and not for human or veterinary use.
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| References | |
| Additional Infomation |
Rhodamine 101 chloride (Rhodamine 640 chloride) is a highly fluorescent xanthene dye used primarily as a research tool in biological imaging and detection applications. The compound has a molecular formula of C32H31ClN2O3 and a molecular weight of 527.05. It is one of the brightest fluorescent dyes and is often used as a reference standard for labeled bioconjugates. The dye has excitation and emission maxima of 565 nm and 595 nm, respectively. Rhodamine 101 chloride is widely used in microscopy, flow cytometry, fluorescence correlation spectroscopy, and ELISA. The compound is not approved for clinical use and is available only for research purposes.
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| Molecular Formula |
C32H31N2O3+.CL-
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|---|---|
| Molecular Weight |
527.05314
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| Exact Mass |
526.202
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| CAS # |
64339-18-0
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| PubChem CID |
14844402
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| Appearance |
Pale purple to purple solid powder
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| Melting Point |
>300ºC (decomposes)
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| LogP |
3.763
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
38
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| Complexity |
1080
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C1CC2=CC3=C(C4=C2N(C1)CCC4)OC5=C6CCC[N+]7=C6C(=CC5=C3C8=CC=CC=C8C(=O)O)CCC7.[Cl-]
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| InChi Key |
ANORACDFPHMJSX-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C32H30N2O3.ClH/c35-32(36)22-10-2-1-9-21(22)27-25-17-19-7-3-13-33-15-5-11-23(28(19)33)30(25)37-31-24-12-6-16-34-14-4-8-20(29(24)34)18-26(27)31;/h1-2,9-10,17-18H,3-8,11-16H2;1H
|
| Chemical Name |
2-(3-oxa-23-aza-9-azoniaheptacyclo[17.7.1.15,9.02,17.04,15.023,27.013,28]octacosa-1(27),2(17),4,9(28),13,15,18-heptaen-16-yl)benzoic acid;chloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
Ethanol : ~14.29 mg/mL (~27.11 mM)
H2O : ~2 mg/mL (~3.79 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.43 mg/mL (2.71 mM) (saturation unknown) in 10% EtOH + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 14.3 mg/mL clear EtOH stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.43 mg/mL (2.71 mM) (saturation unknown) in 10% EtOH + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 14.3 mg/mL clear EtOH stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 1.43 mg/mL (2.71 mM) (saturation unknown) in 10% EtOH + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.8974 mL | 9.4868 mL | 18.9735 mL | |
| 5 mM | 0.3795 mL | 1.8974 mL | 3.7947 mL | |
| 10 mM | 0.1897 mL | 0.9487 mL | 1.8974 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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