| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
D-Glutamic acid-d5 does not have a specific biological target as it is an analytical standard. Its non-deuterated form, D-glutamic acid, is an enantiomer of L-glutamic acid and is found in bacterial cell walls. D-glutamic acid is not a mammalian neurotransmitter but can be metabolized by enzymes such as D-amino acid oxidase. The labeled compound is used as an internal standard for the quantification of D-glutamic acid in biological samples, not for evaluating biological activity against specific targets.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
The labeled compound itself does not possess intrinsic pharmacological activity in vitro. D-Glutamic acid is a non-essential amino acid that is not typically used in mammalian cell culture as a primary nutrient. In bacterial systems, D-glutamic acid is a component of peptidoglycan. The labeled compound is used as an analytical standard for the quantification of D-glutamic acid in biological samples, not for evaluating its own biological activity. |
| ln Vivo |
D-Glutamic acid-d5 is not used as a therapeutic agent; its non-deuterated form, D-glutamic acid, is a naturally occurring amino acid found in bacteria. In vivo, D-glutamic acid is metabolized by D-amino acid oxidase and other enzymes. It does not exert significant pharmacological effects in mammals. The labeled compound is used as an internal standard for the quantification of D-glutamic acid in biological samples for research purposes.
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| Enzyme Assay |
In vitro assays for D-glutamic acid-d5 focus on its use as an analytical standard. A standard protocol involves preparing a solution of the compound in an appropriate solvent (e.g., water or methanol) and using it as an internal standard for GC- or LC-MS analysis of D-glutamic acid in biological samples (e.g., plasma, urine, or bacterial cultures). The compound is added to samples before derivatization and analysis. Quantification is performed by monitoring specific mass transitions for the labeled and unlabeled compounds.
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| Cell Assay |
In vitro cell culture experiments are not performed with D-glutamic acid-d5. When studying the effects of D-glutamic acid in cellular systems, the non-labeled compound is used. Bacterial cultures are treated with D-glutamic acid, and cell wall synthesis or metabolism is measured. The labeled compound is used as an internal standard for LC-MS analysis of D-glutamic acid concentrations in cell culture media or lysates.
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| Animal Protocol |
In vivo animal studies are not conducted with D-glutamic acid-d5. When used in pharmacokinetic studies, the labeled compound serves as an internal standard for the quantification of D-glutamic acid in animal plasma or tissue samples. In typical protocols, animals are administered D-glutamic acid, and blood samples are collected at various time points. The labeled internal standard is added to the samples before analysis by GC- or LC-MS to ensure accurate quantification.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of D-glutamic acid-d5 itself are not characterized, as it is not a drug substance. D-Glutamic acid is metabolized by D-amino acid oxidase and other enzymes. Its half-life in circulation is short. The labeled compound is used as an internal standard for the quantification of D-glutamic acid in pharmacokinetic studies, not as a drug candidate. It is not intended for human exposure.
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| Toxicity/Toxicokinetics |
D-Glutamic acid-d5 is not a therapeutic agent and has not been evaluated for toxicity in humans. Its non-deuterated form, D-glutamic acid, is a naturally occurring amino acid with low toxicity. The labeled compound is for research use only and should be handled with standard laboratory precautions, including the use of gloves and safety glasses.
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| References | |
| Additional Infomation |
D-Glutamic acid-d5 is a stable isotope-labeled internal standard used for the quantification of D-glutamic acid in biological samples. It is also known as D-glutamic-2,3,3,4,4-d5 acid. The compound is used in analytical method development, quality control, and metabolic studies. The incorporation of deuterium allows for accurate quantification of D-glutamic acid in biological samples using mass spectrometry.
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| Molecular Formula |
C5H4D5NO4
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|---|---|
| Molecular Weight |
152.16
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| Exact Mass |
152.084
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| CAS # |
14341-88-9
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| Related CAS # |
D-Glutamic acid;6893-26-1
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| PubChem CID |
56845930
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| Appearance |
White to off-white solid powder
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| Density |
1.4±0.1 g/cm3
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| Boiling Point |
333.8±32.0 °C at 760 mmHg
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| Flash Point |
155.7±25.1 °C
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| Vapour Pressure |
0.0±1.5 mmHg at 25°C
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| Index of Refraction |
1.522
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| LogP |
-1.43
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
10
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| Complexity |
145
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| Defined Atom Stereocenter Count |
1
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| SMILES |
[2H][C@](C(=O)O)(C([2H])([2H])C([2H])([2H])C(=O)O)N
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| InChi Key |
WHUUTDBJXJRKMK-XSDLJHQPSA-N
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| InChi Code |
InChI=1S/C5H9NO4/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H,7,8)(H,9,10)/t3-/m1/s1/i1D2,2D2,3D
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| Chemical Name |
(2R)-2-amino-2,3,3,4,4-pentadeuteriopentanedioic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.5720 mL | 32.8601 mL | 65.7203 mL | |
| 5 mM | 1.3144 mL | 6.5720 mL | 13.1441 mL | |
| 10 mM | 0.6572 mL | 3.2860 mL | 6.5720 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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