| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| Other Sizes |
| Targets |
Multiple targets including antioxidant enzymes, inflammatory mediators, and signaling pathways. Delphinidin-3-O-arabinoside chloride acts as a potent antioxidant by scavenging free radicals and reducing oxidative stress. The anthocyanin structure, particularly the presence of multiple hydroxyl groups on the B-ring, enables the compound to neutralize reactive oxygen species (ROS) and reactive nitrogen species (RNS). The compound also exhibits anti-inflammatory activity by modulating inflammatory signaling pathways, including the NF-κB pathway, and reducing the production of pro-inflammatory cytokines. Its cardioprotective effects are mediated through the inhibition of LDL oxidation and improvement of endothelial function. The compound's anticancer activity involves the induction of apoptosis and inhibition of cell proliferation.
|
|---|---|
| ln Vitro |
Delphinidin-3-O-arabinoside chloride exhibits significant antioxidant activity. It has been shown to scavenge free radicals and reduce oxidative stress in various in vitro systems. The compound demonstrates DPPH radical-scavenging capacity, ABTS radical scavenging capacity, reducing power, and oxygen radical absorbance capacity (ORAC). It has significantly higher antioxidant activity compared to Fe²⁺ controls. The compound also shows anti-inflammatory activity by reducing the production of inflammatory mediators. Its antimicrobial activity has been demonstrated against various bacterial and fungal strains. The compound's anticancer effects include inhibition of cancer cell proliferation and induction of apoptosis in various cancer cell lines.
|
| ln Vivo |
In vivo studies of delphinidin-3-O-arabinoside chloride are limited. As an anthocyanin, it is expected to be absorbed after oral administration and distributed to various tissues. Anthocyanins are known to undergo extensive metabolism, including deglycosylation, glucuronidation, and sulfation, and are eliminated primarily in urine and bile. The compound's antioxidant and anti-inflammatory activities suggest potential benefits in animal models of oxidative stress, inflammation, and metabolic disease. Further studies are needed to characterize its specific in vivo pharmacokinetic and pharmacodynamic properties.
|
| Enzyme Assay |
Non-cell-based assays for delphinidin-3-O-arabinoside chloride include standard antioxidant assays such as DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging, ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) radical scavenging, ferric reducing antioxidant power (FRAP), and oxygen radical absorbance capacity (ORAC) assays. These assays measure the compound's ability to scavenge free radicals or reduce oxidants in cell-free systems. For compound characterization, standard analytical methods including HPLC-UV, LC-MS/MS, and NMR are used to confirm identity and purity. The compound's stability under various conditions (pH, temperature, light) can be assessed using spectrophotometric methods.
|
| Cell Assay |
Cell-based assays for delphinidin-3-O-arabinoside chloride use various cell lines to assess its biological activities. For antioxidant studies, cells are exposed to oxidative stress inducers (e.g., H₂O₂) and treated with the compound, and intracellular ROS levels are measured using fluorescent probes such as DCFH-DA. For anti-inflammatory studies, macrophage cell lines (e.g., RAW 264.7) are stimulated with LPS and treated with the compound, and inflammatory mediators (NO, TNF-α, IL-6) in culture supernatant are measured. For anticancer studies, cancer cell lines are treated with the compound and cell viability (MTT), apoptosis (Annexin V/PI), and cell cycle progression are assessed.
|
| Animal Protocol |
In vivo studies of delphinidin-3-O-arabinoside chloride are limited. Based on its biological activities, potential animal models include: DSS-induced colitis models for anti-inflammatory evaluation; high-fat diet-induced obesity models for anti-adipogenesis and metabolic studies; and tumor xenograft models for anticancer evaluation. Standard protocols for these models involve administration of the compound (oral, intraperitoneal, or intravenous) followed by measurement of relevant endpoints including inflammatory markers, metabolic parameters, tumor volume, and histopathological analysis.
|
| ADME/Pharmacokinetics |
Delphinidin-3-O-arabinoside chloride has a molecular weight of 470.81 g/mol and a molecular formula of C₂₀H₁₉ClO₁₁. The SMILES notation is provided. The compound should be stored as a powder at -20°C for up to 3 years or in solvent at -80°C for 1 year. It can be shipped with blue ice or at ambient temperature. The compound is soluble in appropriate solvents for in vitro and in vivo studies. For in vivo formulation, standard protocols for anthocyanins can be used. The product can be stored for up to 24 months at 2-8°C if stored as stated on the product vial.
|
| Toxicity/Toxicokinetics |
Toxicological data for delphinidin-3-O-arabinoside chloride are limited. Anthocyanins are generally considered to have low toxicity and are widely consumed in the human diet as components of fruits and vegetables. No significant toxicity has been reported at dietary levels. High doses may cause gastrointestinal effects. The compound is intended for research use only. Standard laboratory safety practices should be followed when handling this compound, including the use of personal protective equipment.
|
| References | |
| Additional Infomation |
Delphinidin-3-O-arabinoside is a glycoside of flavonoids.
Delphinidin-3-O-arabinoside chloride is a naturally occurring anthocyanin found in various fruits and vegetables, including Saskatoon berries and other plant sources. Anthocyanins are water-soluble pigments responsible for the red, purple, and blue colors of many fruits and vegetables. They are known for their potent antioxidant and health-promoting properties. Delphinidin-3-O-arabinoside chloride is used as a reference standard for the identification and quantification of anthocyanins in plant extracts and food products. Its biological activities, including antioxidant, anti-inflammatory, and anticancer effects, make it a compound of interest for nutritional and pharmacological research. It is for research use only. |
| Molecular Formula |
C20H19CLO11
|
|---|---|
| Molecular Weight |
470.81
|
| Exact Mass |
470.061
|
| CAS # |
171370-55-1
|
| PubChem CID |
91810628
|
| Appearance |
Purple to purplish red solid
|
| Hydrogen Bond Donor Count |
8
|
| Hydrogen Bond Acceptor Count |
11
|
| Rotatable Bond Count |
3
|
| Heavy Atom Count |
32
|
| Complexity |
596
|
| Defined Atom Stereocenter Count |
4
|
| SMILES |
[Cl-].O1C([H])([H])[C@]([H])([C@]([H])([C@@]([H])([C@@]1([H])OC1=C([H])C2=C(C([H])=C(C([H])=C2[O+]=C1C1C([H])=C(C(=C(C=1[H])O[H])O[H])O[H])O[H])O[H])O[H])O[H])O[H]
|
| InChi Key |
BQQCUFJAUJKCKH-GOWHUIJJSA-N
|
| InChi Code |
InChI=1S/C20H18O11.ClH/c21-8-3-10(22)9-5-15(31-20-18(28)17(27)13(25)6-29-20)19(30-14(9)4-8)7-1-11(23)16(26)12(24)2-7;/h1-5,13,17-18,20,25,27-28H,6H2,(H4-,21,22,23,24,26);1H/t13-,17-,18+,20-;/m0./s1
|
| Chemical Name |
(2S,3R,4S,5S)-2-[5,7-dihydroxy-2-(3,4,5-trihydroxyphenyl)chromenylium-3-yl]oxyoxane-3,4,5-triol;chloride
|
| Synonyms |
Delphinidin-3-O-arabinoside chloride
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1240 mL | 10.6200 mL | 21.2400 mL | |
| 5 mM | 0.4248 mL | 2.1240 mL | 4.2480 mL | |
| 10 mM | 0.2124 mL | 1.0620 mL | 2.1240 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
