| Size | Price | Stock | Qty |
|---|---|---|---|
| 50mg |
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| Other Sizes |
| Targets |
Phosphoglycerol transferase (competitive inhibitor); β-glucan-related enzymes. β-Gentiobiose is a competitive inhibitor of phosphoglycerol transferase. It is also used in binding assays to determine if proteins bind to β-1,3-glucans. As a disaccharide component of β-glucans, it is important for investigating the enzymatic breakdown and synthesis of β-glucans.
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|---|---|
| ln Vitro |
β-Gentiobiose is a competitive inhibitor of phosphoglycerol transferase. It is a natural oligosaccharide that promotes fruit ripening. The compound is used in binding assays to determine if coelomic cytolytic factor 1 (CCF-1) binds to β-1,3-glucans. Its in vitro activity includes serving as a substrate or inhibitor in enzymatic studies of carbohydrate metabolism.
|
| ln Vivo |
In vivo, β-gentiobiose promotes fruit ripening. It is a natural oligosaccharide found in various plant sources. The compound is used in research to study carbohydrate metabolism, β-glucan synthesis and degradation, and plant physiology. It is not administered as a therapeutic agent but serves as a research tool for studying carbohydrate-related processes.
|
| Enzyme Assay |
Non-cell-based assays for β-gentiobiose include enzyme inhibition studies using phosphoglycerol transferase or other carbohydrate-metabolizing enzymes. The compound is incubated with the enzyme and substrate, and enzyme activity is measured by detecting product formation. Competitive inhibition is determined by varying substrate concentrations in the presence of fixed inhibitor concentrations. Binding assays are used to study interactions with β-glucan-binding proteins.
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| Cell Assay |
Cell-based assays for β-gentiobiose are limited, as the compound is primarily used in biochemical and enzymatic studies. In plant cell culture models, the compound’s effects on fruit ripening and cell wall metabolism can be assessed. In microbial culture models, its effects on β-glucan metabolism and microbial growth can be studied.
|
| Animal Protocol |
In vivo studies of β-gentiobiose are conducted in plant models to study fruit ripening and carbohydrate metabolism. The compound may be applied to fruits or plants, and ripening parameters, sugar content, and enzyme activities are measured. In animal models, the compound’s effects on carbohydrate metabolism and gut microbiota can be studied.
|
| ADME/Pharmacokinetics |
β-Gentiobiose has a molecular formula of C₁₂H₂₂O₁₁ and a molecular weight of 342.30 g/mol. It is a natural disaccharide composed of two D-glucose units linked by a β-1,6-glycosidic bond. Purity is ≥97%. The compound should be stored under recommended conditions. It is intended for research use only.
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| Toxicity/Toxicokinetics |
β-Gentiobiose is generally considered to have low toxicity as a natural sugar. It is not intended for human therapeutic use. Standard laboratory safety practices should be followed when handling this compound.
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| References | |
| Additional Infomation |
6-ObD-glucopyranosyl-D-glucose has reportedly been found in Ascochyta medicaginicola, Gentiana straminea, and other organisms with available data.
β-Gentiobiose (gentiobiose) is a natural disaccharide composed of two D-glucose units linked by a β-1,6-glycosidic bond. It is a natural oligosaccharide that promotes fruit ripening and is extensively studied for its role in carbohydrate chemistry and biological research. The sugar is important for investigating the enzymatic breakdown and synthesis of β-glucans, key components of plant and fungal cell walls. It acts as a competitive inhibitor of phosphoglycerol transferase and is used in binding assays for β-glucan-binding proteins. It is for research use only. |
| Molecular Formula |
C12H22O11
|
|---|---|
| Molecular Weight |
342.30
|
| Exact Mass |
342.116
|
| CAS # |
554-91-6
|
| PubChem CID |
20056559
|
| Appearance |
White to off-white solid powder
|
| Density |
1.76 g/cm3
|
| Boiling Point |
662.8ºC at 760 mmHg
|
| Melting Point |
195-197 °C (dec.)
|
| Flash Point |
354.6ºC
|
| Index of Refraction |
9.5 ° (C=4, H2O)
|
| LogP |
-5
|
| Hydrogen Bond Donor Count |
8
|
| Hydrogen Bond Acceptor Count |
11
|
| Rotatable Bond Count |
8
|
| Heavy Atom Count |
23
|
| Complexity |
367
|
| Defined Atom Stereocenter Count |
9
|
| SMILES |
C([C@@H]1[C@H]([C@@H]([C@H]([C@@H](O1)OC[C@H]([C@H]([C@@H]([C@H](C=O)O)O)O)O)O)O)O)O
|
| InChi Key |
AYRXSINWFIIFAE-UDKQPYHCSA-N
|
| InChi Code |
InChI=1S/C12H22O11/c13-1-4(15)7(17)8(18)5(16)3-22-12-11(21)10(20)9(19)6(2-14)23-12/h1,4-12,14-21H,2-3H2/t4-,5+,6+,7+,8+,9+,10-,11+,12+/m0/s1
|
| Chemical Name |
(2R,3S,4R,5R)-2,3,4,5-tetrahydroxy-6-[(2R,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyhexanal
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O: 33.33 mg/mL (97.37 mM)
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9214 mL | 14.6071 mL | 29.2141 mL | |
| 5 mM | 0.5843 mL | 2.9214 mL | 5.8428 mL | |
| 10 mM | 0.2921 mL | 1.4607 mL | 2.9214 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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