| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| Other Sizes |
| Targets |
Daidzein metabolite
Daidzein-4',7-diglucoside does not have specific pharmacological targets, as it is a natural product used as a reference standard rather than a therapeutic agent. As an isoflavone diglycoside, it may share biological activities with other isoflavones, including estrogenic or anti-estrogenic effects. However, the diglycosylated form is expected to have limited biological activity due to poor cellular uptake. |
|---|---|
| ln Vitro |
Daidzein-4,7-diglucoside has been reported in Maackia amurensis.
Daidzein-4',7-diglucoside is not evaluated for traditional biological activity, as it is a natural product used as a reference standard. As an isoflavone diglycoside, it may serve as a reference for the complete profiling of soy isoflavones. The compound is utilized in the study of plant secondary metabolism and solubility. |
| ln Vivo |
The dose effect of pure daidzin on the suppression of ethanol intake in Syrian golden hamsters was compared with that of crude daidzin contained in a methanol extract of Radix puerariae (RP). EC50 values estimated from the graded dose-response curves for pure daidzin and RP extract daidzin are 23 and 2.3 mg per hamster per day, respectively. Apparently the antidipsotropic activity of the RP extract cannot be accounted for solely by its daidzin content (22 mg/g). In addition to daidzin, six other isoflavones were identified in the RP extract and quantified--namely, puerarin (160 mg per g of extract), genistin (3.7 mg/g), daidzein (2.6 mg/g), daidzein-4',7-diglucoside (1.2 mg/g), genistein (0.2 mg/g), and formononetin (0.16 mg/g). None of these, administered either alone or combined, contributes in any significant way to the antidipsotropic activity of the extract. Plasma daidzin concentration-time curves determined in hamsters administered various doses of pure daidzin or RP extract by i.p.injection indicate that the crude extract daidzin has approximately 10 times greater bioavailability than the pure compound. Reconstruction of the dose-response effects for pure and crude daidzin using bioavailable daidzin rather than administered dose gives a single curve. Synthetic daidzin added to the RP extract acquires the bioavailability of the endogenous daidzin that exists naturally in the extract. These results show that (i) daidzin is the major active principle in methanol extracts of RP, and (ii) additional constituents in the methanol extract of RP assist uptake of daidzin in golden hamsters.[1]
In vivo activity data for Daidzein-4',7-diglucoside are not available in the published literature. The compound has been primarily characterized as a natural product from Radix puerariae and other plants. No in vivo pharmacological studies have been reported for this compound. Its applications are limited to analytical and phytochemical research. |
| Enzyme Assay |
In vitro assays for Daidzein-4',7-diglucoside typically involve analytical methods for its detection and quantification in plant extracts or biological samples. HPLC or LC-MS methods are used to measure the compound's concentration. The compound is used as a reference standard for the profiling of soy isoflavones. Its structure can be confirmed by NMR and MS. Purity is assessed by HPLC.
|
| Cell Assay |
In vitro cellular assays for Daidzein-4',7-diglucoside are not typically performed, as the compound is used as a reference standard rather than a cellular modulator. It may be used in studies of isoflavone metabolism or transport. Cells are treated with the compound and its uptake, metabolism, or biological effects are assessed. However, such studies are not common for this compound.
|
| Animal Protocol |
In vivo animal experiments for Daidzein-4',7-diglucoside have not been reported in the published literature. The compound has been studied primarily as a natural product from Radix puerariae and other plants. No animal studies have been conducted to evaluate its pharmacological effects.
|
| ADME/Pharmacokinetics |
Pharmacokinetic properties of Daidzein-4',7-diglucoside have not been extensively characterized. The compound has a molecular weight of 578.52 g/mol and a molecular formula of C₂₇H₃₀O₁₄. As a diglycosylated isoflavone, it is expected to have very limited oral bioavailability due to its large size and hydrophilic nature. The compound may undergo deglycosylation in the gastrointestinal tract. Storage: 4°C, sealed, away from moisture and light.
|
| Toxicity/Toxicokinetics |
The toxicological profile of Daidzein-4',7-diglucoside has not been extensively documented. As a naturally occurring isoflavone diglycoside from edible plants, it is expected to have a favorable safety profile. Standard laboratory safety precautions should be followed when handling this compound.
|
| References | |
| Additional Infomation |
According to reports, Maackia amurensis contains daidzein-4,7-diglucoside, and there is relevant data available.
Daidzein-4',7-diglucoside (CAS# 53681-67-7, molecular formula C₂₇H₃₀O₁₄, molecular weight 578.52) is a naturally occurring diglycosylated isoflavone from Radix puerariae and other plants. Used as a reference standard for soy isoflavone profiling. No clinical trials or regulatory approvals have been identified. |
| Molecular Formula |
C27H30O14
|
|---|---|
| Molecular Weight |
578.52
|
| Exact Mass |
578.164
|
| CAS # |
53681-67-7
|
| PubChem CID |
171292
|
| Appearance |
White to off-white solid powder
|
| Source |
Radix puerariae
|
| LogP |
-1.1
|
| Hydrogen Bond Donor Count |
8
|
| Hydrogen Bond Acceptor Count |
14
|
| Rotatable Bond Count |
7
|
| Heavy Atom Count |
41
|
| Complexity |
924
|
| Defined Atom Stereocenter Count |
10
|
| SMILES |
O1[C@H]([C@@H]([C@H]([C@@H]([C@H]1CO)O)O)O)OC1=CC=C2C(C(=COC2=C1)C1C=CC(=CC=1)O[C@H]1[C@@H]([C@H]([C@@H]([C@@H](CO)O1)O)O)O)=O
|
| InChi Key |
VWEWSCDQMVNOJP-IPOZFMEPSA-N
|
| InChi Code |
InChI=1S/C27H30O14/c28-8-17-20(31)22(33)24(35)26(40-17)38-12-3-1-11(2-4-12)15-10-37-16-7-13(5-6-14(16)19(15)30)39-27-25(36)23(34)21(32)18(9-29)41-27/h1-7,10,17-18,20-29,31-36H,8-9H2/t17-,18-,20-,21-,22+,23+,24-,25-,26-,27-/m1/s1
|
| Chemical Name |
7-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-3-[4-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyphenyl]chromen-4-one
|
| Synonyms |
Daidzein-4,7-diglucoside; 53681-67-7; daidzein-4',7-diglucoside; 7-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-3-[4-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyphenyl]chromen-4-one; Daidzein 7,4'-di-O-glucoside; DTXSID00201934;
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.7285 mL | 8.6427 mL | 17.2855 mL | |
| 5 mM | 0.3457 mL | 1.7285 mL | 3.4571 mL | |
| 10 mM | 0.1729 mL | 0.8643 mL | 1.7285 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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