| Size | Price | Stock | Qty |
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| 5mg |
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| Other Sizes |
| Targets |
5-Hydroxy-7-acetoxy-8-methoxyflavone targets lipid peroxidation pathways, inhibiting the oxidative degradation of lipids. As a flavonoid, it may exert antioxidant effects by scavenging free radicals and chelating metal ions. Its potential anti-influenza virus activity suggests interactions with viral proteins or host cell factors involved in viral replication. The compound's methoxy and acetoxy substitutions on the flavonoid backbone contribute to its bioactivity profile.
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| ln Vitro |
In vitro, 5-Hydroxy-7-acetoxy-8-methoxyflavone inhibits lipid peroxidation. It may exhibit anti-influenza virus activity. As a flavonoid, it is expected to have antioxidant properties, though specific IC50 values are not detailed in the available literature. The compound's activity is typically assessed using standard antioxidant and antiviral assays. Further studies are needed to fully characterize its in vitro biological activities.
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| ln Vivo |
Specific in vivo data for 5-Hydroxy-7-acetoxy-8-methoxyflavone are not reported in the available literature. Given its in vitro inhibition of lipid peroxidation and potential anti-influenza virus activity, the compound has potential for in vivo studies in models of oxidative stress-related diseases and viral infections. However, specific published in vivo protocols for this compound are not available.
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| Enzyme Assay |
The lipid peroxidation inhibitory activity is assessed using standard in vitro assays. Liposomes or liver microsomes are incubated with a pro-oxidant (e.g., Fe²⁺/ascorbate or AAPH) and various concentrations of 5-Hydroxy-7-acetoxy-8-methoxyflavone. Lipid peroxidation is measured by the thiobarbituric acid reactive substances (TBARS) assay or by monitoring conjugated diene formation spectrophotometrically. The percentage inhibition is calculated compared to control samples without the compound.
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| Cell Assay |
For cellular studies, appropriate cell lines (e.g., hepatocytes, lung epithelial cells) are cultured in suitable media. Cells are treated with 5-Hydroxy-7-acetoxy-8-methoxyflavone at various concentrations (e.g., 1-100 μM) for 24-72 hours. Cell viability is assessed using MTT or similar assays. For antiviral studies, virus-infected cells are treated with the compound, and viral replication is measured by plaque assay or qPCR. The compound is typically dissolved in DMSO and diluted in culture media.
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| Animal Protocol |
In vivo studies for 5-Hydroxy-7-acetoxy-8-methoxyflavone would be conducted in appropriate animal models. For antiviral studies, influenza virus-infected mouse models would be used, with the compound administered via oral gavage or intraperitoneal injection. For oxidative stress studies, models of liver injury or inflammation would be employed. However, specific published protocols for this compound are not available.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for 5-Hydroxy-7-acetoxy-8-methoxyflavone are not reported. The compound has a molecular formula of C₁₈H₁₄O₆ and a molecular weight of 326.30 g/mol. It is typically stored as a solid at room temperature. Pharmacokinetic studies would be required to determine parameters such as absorption, distribution, metabolism, and excretion. The compound has a purity of ≥98%.
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| Toxicity/Toxicokinetics |
Toxicological data for 5-Hydroxy-7-acetoxy-8-methoxyflavone are not reported. As a flavonoid compound, it is generally considered to have a moderate safety profile based on the known safety of dietary flavonoids. However, comprehensive toxicology studies have not been published. The compound should be handled with appropriate safety precautions in laboratory settings.
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| References | |
| Additional Infomation |
5-Hydroxy-7-acetoxy-8-methoxyflavone is a flavonoid that inhibits lipid peroxidation and may exhibit anti-influenza virus activity. Molecular formula: C₁₈H₁₄O₆, molecular weight: 326.30. Purity ≥98%. No clinical trials exist. For research use only.
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| Molecular Formula |
C18H14O6
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|---|---|
| Molecular Weight |
326.30
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| Exact Mass |
326.079
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| CAS # |
95480-80-1
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| PubChem CID |
21721889
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| Appearance |
Typically exists as solid at room temperature
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| Density |
1.4±0.1 g/cm3
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| Boiling Point |
536.9±50.0 °C at 760 mmHg
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| Flash Point |
198.3±23.6 °C
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| Vapour Pressure |
0.0±1.5 mmHg at 25°C
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| Index of Refraction |
1.620
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| LogP |
2.23
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
24
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| Complexity |
522
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O=C1C2C(=C(C(=CC=2O)OC(C)=O)OC)OC(C2C=CC=CC=2)=C1
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| InChi Key |
QDRDFENYJDAHOT-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C18H14O6/c1-10(19)23-15-9-13(21)16-12(20)8-14(11-6-4-3-5-7-11)24-18(16)17(15)22-2/h3-9,21H,1-2H3
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| Chemical Name |
(5-hydroxy-8-methoxy-4-oxo-2-phenylchromen-7-yl) acetate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.0647 mL | 15.3233 mL | 30.6466 mL | |
| 5 mM | 0.6129 mL | 3.0647 mL | 6.1293 mL | |
| 10 mM | 0.3065 mL | 1.5323 mL | 3.0647 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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