| Size | Price | Stock | Qty |
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| 10mg |
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| Other Sizes |
| ln Vitro |
QBS10072S (30 min) dihydrochloride dose-dependently reduced cell viability in glioblastoma (GBM) cell lines (U251, LN229, 8MGBA, 42MGBA, DBTRG, AM38) with EC50 values ranging from 5.0 to 40 μM[1]. QBS10072S (16 h) dihydrochloride dose-dependently induced DNA damage in U251 and LN229 GBM cells after 16 h of treatment, but no DNA damage was observed in normal human astrocytes (NHA)[1]. QBS10072S (one week) dihydrochloride dose-dependently reduced cell viability in LAT1-positive H9, HH, MJ, and Hut78 T-cell lymphoma cell lines with IC50 values ranging from 4.4 μM (Hut78) to 51 μM (HH)[2].
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| ln Vivo |
QBS10072S (10 mg/kg; intravenous injection; once a week; 4–6 weeks) dihydrochloride inhibited the growth of U251 glioblastoma[1]. QBS10072S (2–8 mg/kg; intravenous injection; once every two weeks; three times in total) dihydrochloride inhibited the growth of LN229 glioblastoma[1]. QBS10072S (8 mg/kg) dihydrochloride showed significant antitumor activity in a mouse H9 T-cell lymphoma xenograft model[2].
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| Animal Protocol |
Animal/Disease Models:Athymic nude mice (female, 5 weeks old, intracranial injection of U251 human glioblastoma cells expressing luciferase) [1]
Doses: 10 mg/kg Route of Administration: Intravenous injection; once a week; for 4 and 6 weeks Experimental Results: 10 mg/kg weekly for 6 weeks achieved a 95% tumor growth inhibition rate (TGI) on day 35 and extended median survival from 37 days (vector group) to 70 days. 10 mg/kg weekly for 4 weeks achieved an 86% TGI on day 35 and extended median survival to 45 days. Animal/Disease Models:Athymic nude mice (female, 5 weeks old, intracranial injection of LN229 human glioblastoma cells expressing luciferase)[1] Doses: 2, 4 and 8 mg/kg Route of Administration: Intravenous injection; once every two weeks; three times in total Experimental Results: Induction of dose-dependent tumor growth inhibition: At day 53, the tumor growth inhibition rate was 72% in the 2 mg/kg group, 73% in the 4 mg/kg group and 84% in the 8 mg/kg group; median survival was extended from 56 days (vector) to 66.5 days (2 mg/kg), 71 days (4 mg/kg) and 74 days (8 mg/kg). |
| References |
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| Molecular Formula |
C15H24CL4N2O2
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|---|---|
| Molecular Weight |
406.18
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| CAS # |
1802735-31-4
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| Appearance |
Typically exists as solids at room temperature
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| SMILES |
ClCCN(C1=CC(C[C@H](N)CC(O)=O)=C(C=C1)C)CCCl.Cl.Cl
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4620 mL | 12.3098 mL | 24.6196 mL | |
| 5 mM | 0.4924 mL | 2.4620 mL | 4.9239 mL | |
| 10 mM | 0.2462 mL | 1.2310 mL | 2.4620 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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