| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| ln Vivo |
Magnesium curare (0.25–1 mg/10 g; via peritoneal lymph sac injection; single dose) can induce dose-dependent neuromuscular paralysis in frogs. A dose of 0.75 mg/10 g can cause complete respiratory paralysis (lasting 165 minutes), while a dose of 1 mg/10 g has no effect on spinal multineuronal reflexes [1]. Magnesium curare (5–15.63 mg/kg; intravenous injection; single dose) can induce dose-dependent neuromuscular blockade and respiratory effects in rabbits anesthetized with ethyl carbamate. A dose of 15 mg/kg can induce complete muscle paralysis and respiratory arrest, with animals fully recovering within 25–30 minutes [1].
|
|---|---|
| Animal Protocol |
Animal/Disease Models:Frogs collected in autumn [1]
Doses: 0.25 mg/10g; 0.5 mg/10g; 0.75 mg/10g; 1.0 mg/10g; 1 mg/10g Route of Administration: Ventral lymph sac; Single dose Experimental Results: At a dose of 0.25 mg/10g, the righting reflex was weakened but not completely eliminated. At a dose of 0.5 mg/10g, the righting reflex disappeared, but the skin reflex was weakened, and respiratory movements were visible. At a dose of 0.75 mg/10g, all movements (including respiration) stopped, and paralysis lasted for 165 minutes. At a dose of 1.0 mg/10g, complete paralysis was caused, lasting for 330 minutes. Twenty-five minutes after injection of 0.75 mg/10g of the drug into the unligated lower limb, the contractile response of the gastrocnemius muscle to sciatic nerve stimulation was completely blocked, while the ligated (untreated) lower limb remained responsive. At a dose of 1 mg/10g, the drug did not inhibit spinal multineuronal reflexes because even with complete limb paralysis, the gastrocnemius muscle of the untreated (ligated) lower limb could still contract upon sciatic nerve stimulation. Animal/Disease Models:Rabbits (2.0-2.5 kg; ethyl carbamate anesthesia) [1] Doses: 15.63 mg/kg (head drop); 5 mg/kg; 10 mg/kg; 15 mg/kg Route of Administration: Intravenous injection; single dose (within 5 seconds); intravenous injection; 2 mg/15 seconds infusion Experimental Results: The 15.63 mg/kg dose (2 mg/15 seconds administration) caused head drop. The 5 mg/kg dose reduced the strength of gastrocnemius muscle contraction for 5-8 minutes, resulting in early respiratory depression, followed by respiratory stimulation lasting 8-10 minutes. The 10 mg/kg dose significantly inhibited muscle spasms and respiration more than the 5 mg/kg dose, and for a longer duration. Within 1 minute after injection, it completely inhibits gastrocnemius muscle contraction and stops respiratory movement. Muscle response and spontaneous breathing begin to recover 7-8 minutes after injection, and complete recovery occurs within 25-30 minutes at a dose of 15 mg/kg. |
| References |
| Molecular Formula |
C19H24CLNO3
|
|---|---|
| Molecular Weight |
349.85
|
| CAS # |
75413-87-5
|
| Appearance |
Typically exists as solids at room temperature
|
| SMILES |
COC(C=C(CC[N+]1(C)C)C([C@H]1CC2=CC=C(C=C2)O)=C3)=C3O.[Cl-]
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.8584 mL | 14.2918 mL | 28.5837 mL | |
| 5 mM | 0.5717 mL | 2.8584 mL | 5.7167 mL | |
| 10 mM | 0.2858 mL | 1.4292 mL | 2.8584 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
