| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| ln Vitro |
At concentrations ≤25 μg/mL, Ephemeranthol A (6.25-50 μg/mL; 25 h) showed no cytotoxicity to Raw 264.7 cells, but at a concentration of 50 μg/mL, it reduced cell viability [1]. Ephemeranthol A (6.25-50 μg/mL; 7-25 h) effectively inhibited LPS-induced production of NO, iNOS, COX-2, TNF-α, IL-6, and IL-1β in Raw 264.7 cells [1]. Ephemeranthol A (25 μg/mL; 1.5-2 h) blocked LPS-induced IκB degradation and inhibited nuclear translocation of NF-κB subunits p50 and p65 [1]. Centipede alcohol A (6.25-25 μg/mL; 1 h) inhibited LPS-induced phosphorylation of p38 and JNK in Raw 264.7 cells [1]. Centipede alcohol A (5-200 μM; 24-48 h) reduced the viability of human non-small cell lung cancer H460 cells in a concentration- and time-dependent manner, with an IC50 of >200 μM at 24 h and 150.5 μM at 48 h [2]. Centipede alcohol A (10-100 μM; 24 h) induced apoptosis in human non-small cell lung cancer H460 cells in a concentration-dependent manner [2]. Centipede alcohol A (50-100 μM; 48 h) induced apoptosis in human non-small cell lung cancer H460 cells within 48 h by reducing Bcl-2 levels and activating caspase-9, caspase-3 and PARP [2]. Centipede alcohol A (50-100 μM; 48 h) inhibited the activation of the FAK-Akt signaling pathway in human non-small cell lung cancer H460 cells within 48 h [2]. Centipede alcohol A (5-50 μM; 48 h) inhibited the anchorage-independent growth of human non-small cell lung cancer H460 cells[2]. Centipede alcohol A (10-100 μM; 24-48 h) inhibited the migration of human non-small cell lung cancer H460 cells[2]. Centipede alcohol A (5-100 μM; 24-48 h) inhibited epithelial-mesenchymal transition and induced epithelial morphological changes in human non-small cell lung cancer H460 cells[2].
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|---|---|
| Cell Assay |
Western Blot Analysis [1]
Cell Types: Raw 264.7 mouse macrophages Tested Concentrations: 25 μg/mL Incubation Duration: Pre-incubation for 1 hour; LPS stimulation for 6 hours Experimental Results: LPS-induced reduction in iNOS protein production was observed in Raw 264.7 cells. LPS-induced reduction in COX-2 protein production was observed in Raw 264.7 cells. RT-PCR[1] Cell Types: Raw 264.7 mouse macrophages Tested Concentrations: 25 μg/mL Incubation Duration: Pre-incubation for 1 hour; LPS stimulation for 6 hours Experimental Results: Significantly reduced LPS-induced mRNA levels of TNF-α, IL-6, and IL-1β. TNF-α and IL-6 mRNA levels were reduced to those of the unstimulated control group. ELISA detection [1] Cell Types: Raw 264.7 mouse macrophages Tested Concentrations: 25 μg/mL Incubation Duration: Pre-incubation for 1 hour; LPS stimulation for 24 hours Experimental Results: Significantly inhibited the production of LPS-induced TNF-α, IL-6 and IL-1β proteins in Raw 264.7 cells.
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| References |
|
| Molecular Formula |
C16H16O4
|
|---|---|
| Molecular Weight |
272.30
|
| CAS # |
135545-86-7
|
| Appearance |
Typically exists as solids at room temperature
|
| SMILES |
OC1=CC2=C(C3=C(C(OC)=C(C=C3CC2)OC)O)C=C1
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.6724 mL | 18.3621 mL | 36.7242 mL | |
| 5 mM | 0.7345 mL | 3.6724 mL | 7.3448 mL | |
| 10 mM | 0.3672 mL | 1.8362 mL | 3.6724 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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