| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| ln Vitro |
EGFR/BRAFV600E-IN-7 (compound 7c) (50 μM; 4 days) showed no cytotoxicity against human breast epithelial cells MCF-10A, with a cell survival rate of 88%[1]. EGFR/BRAFV600E-IN-7 exhibited potent antiproliferative activity against human colon cancer HT-29 cells (IC50=8 μM), human breast cancer MCF-7 cells (IC50=4 μM), human lung cancer A-549 cells (IC50=6 μM), and human pancreatic cancer Panc-1 cells (IC50=7 μM), with an average GI50 of 6 μM[1]. EGFR/BRAFV600E-IN-7 can activate the intrinsic and extrinsic apoptosis pathways of human breast cancer MCF-7 cells, and compared with the untreated control group, the levels of caspase-3, caspase-8, and caspase-9 proteins increased by 8-fold, 15-fold, and 21-fold, respectively[1]. EGFR/BRAFV600E-IN-7 can also regulate the expression of apoptosis proteins in human breast cancer MCF-7 cells. Compared with the untreated control group, the level of pro-apoptotic protein Bax increased by 27 times and the level of anti-apoptotic protein Bcl-2 decreased by 6 times [1]. EGFR/BRAFV600E-IN-7 (10-100 μM) showed strong antioxidant activity, with DPPH free radical scavenging rate of 90.8% at 100 μM, 80.5% at 50 μM, and 68.4% at 10 μM [1].
|
|---|---|
| Cell Assay |
Cell viability assay [1]
Cell Types: Human mammary epithelial cells MCF-10A Tested Concentrations: 50 μM Incubation Duration: 4 days Experimental Results: 88% of the MCF-10A cells were retained. |
| References |
| Molecular Formula |
C28H21N5O3S
|
|---|---|
| Molecular Weight |
507.56
|
| CAS # |
3120155-78-1
|
| Appearance |
Typically exists as solids at room temperature
|
| SMILES |
O=C(NC1=CC=C(C2=CC(C3=CC=C(OC)C=C3)=C(C#N)C(N2)=O)C=C1)CSC4=NC5=CC=CC=C5N4
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.9702 mL | 9.8511 mL | 19.7021 mL | |
| 5 mM | 0.3940 mL | 1.9702 mL | 3.9404 mL | |
| 10 mM | 0.1970 mL | 0.9851 mL | 1.9702 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
