| Size | Price | Stock | Qty |
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| ln Vitro |
Cyanamide (2-10 mM; 1-6 days) dose-dependently inhibited the growth, fresh weight accumulation, dry weight accumulation, and relative growth rate (RGR) of onion (Allium cepa L.) bulb roots. Complete recovery of growth was observed only after treatment with low concentrations (2 mM), while only partial or no recovery was observed after treatment with high concentrations [1]. Cyanamide (2-10 mM; 1, 6 days) increased H₂O₂ levels in a dose- and time-dependent manner, inducing oxidative stress in onion (Allium cepa L.) bulb roots, with the highest concentration (10 mM) causing a sharp increase in H₂O₂ levels after 1 day of treatment [1]. Cyanamide (2-10 mM; 1-6 days) altered the accumulation of superoxide anions in onion bulb roots. Specifically, treatment with 10 mM cyanamide for 1 day induced a large accumulation of superoxide anions, which in turn triggered tissue degradation [1]. Cyanamide (2-10 mM; 1-6 days) can alter the mitotic index of onion root tip cells, disrupt chromosome morphology and metaphase plate formation, and all mitotic activity and chromosome structure can be completely restored after removal of the compound [1]. Cyanamide (2-10 mM; 1-6 days) can alter the cell cycle distribution of onion root tip cells, increasing the proportion of 4C nuclei after short-term treatment and increasing the G2/G1 ratio during the recovery period [1]. Cyanamide (2-10 mM; 1 day) can disrupt the arrangement of microtubule cytoskeleton in onion root tip meristem cells at concentrations of 6 mM and 10 mM, while no significant effect on microtubule structure was observed at a concentration of 2 mM [1].
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| ln Vivo |
Cyanoamine (70 mg/kg; intraperitoneal injection; 15 minutes before cyclophosphamide administration) enhances the antitumor efficacy of cyclophosphamide in mouse models of hepatocellular carcinoma and lymphosarcoma, extending animal lifespan by at least 80% in the hepatocellular carcinoma model and reducing the cyclophosphamide dose by 2/3 in the lymphosarcoma model while maintaining the same efficacy [2].
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| Cell Assay |
Cell cycle analysis [1]
Cell Types: Onion (Allium cepa L.) root tip cells Tested Concentrations: 2, 6, 10 mM Incubation Duration: 6 days Experimental Results: After short-term treatment, the proportion of 4C cell nuclei increased, and the G2/G1 ratio increased during the recovery period, with the strongest effect at 2 mM. |
| Animal Protocol |
Animal/Disease Models: A/Sn mice (male, intramuscularly injected with HA-1 hepatocellular carcinoma transplantation); CBA mice (male, intramuscularly injected with RLS lymphosarcoma transplantation) [2]
Doses: 70 mg/kg Route of Administration: Intraperitoneal injection; 15 minutes before cyclophosphamide administration Experimental Results: In HA-1 hepatocellular carcinoma A/Sn mice, it significantly inhibited the growth of primary tumor lymph nodes and intrahepatic metastases. It extended the lifespan of HA-1 hepatocellular carcinoma A/Sn mice by at least 80%. In RLS lymphosarcoma CBA mice, its efficacy (significant inhibition of tumor growth and lifespan extension) was comparable to that of cyclophosphamide alone at 3 times the dose. No effect on tumor growth or animal lifespan was shown when administered alone in either model. |
| References |
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| Molecular Formula |
CH2N2
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|---|---|
| Molecular Weight |
42.04
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| CAS # |
420-04-2
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| Appearance |
Liquid (Density: 1.282 g/cm3)
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| SMILES |
N#CN
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: 请将本产品存放在密封且受保护的环境中（例如氮气下），避免暴露在潮湿和光照下。 |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 23.7869 mL | 118.9343 mL | 237.8687 mL | |
| 5 mM | 4.7574 mL | 23.7869 mL | 47.5737 mL | |
| 10 mM | 2.3787 mL | 11.8934 mL | 23.7869 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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