| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| ln Vitro |
Antibiofilm agent-19 (compound 11f) (0.156–2.5 μM; 24 h) effectively inhibited biofilm formation in Pseudomonas aeruginosa PAO1-ΔwspF (0.156 μM) and wild-type PAO1 (2.5 μM) without affecting bacterial growth [1]. Antibiofilm agent-19 (0–2.5 μM; 12–16 h) reduced the production of extracellular polymers in Pseudomonas aeruginosa, with the most significant effect in PAO1-ΔwspF strains with high c-di-GMP levels [1]. Antibiofilm agent-19 (0.125–50 μM; 24 h) disrupted the iron homeostasis of Pseudomonas aeruginosa by promoting siderophore secretion, and this effect could be reversed by the addition of excess Fe3+ [1]. Antibiofilm agent-19 (6.25-200 μM; 24 hours) showed excellent biocompatibility and no significant cytotoxicity to Vero cells or RAW264.7 macrophages even at concentrations up to 200 μM [1].
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|---|---|
| ln Vivo |
Antibiofilm agent-19 (0.156–2.5 μM; intracavitary injection; single dose) in combination with tobramycin or ciprofloxacin can significantly improve the survival rate of large wax moth during infection with Pseudomonas aeruginosa PAO1/PAO1-ΔwspF[1].
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| Cell Assay |
Cytotoxicity assay [1]
Cell Types: Vero cells, RAW264.7 macrophages Tested Concentrations: 6.25, 12.5, 25, 50, 100 and 200 μM Incubation Duration: 24 hours Experimental Results: At all test concentrations, the survival rate of Vero cells and RAW264.7 macrophages was >90%, and no morphological changes were observed. |
| Animal Protocol |
Animal/Disease Models:Wax moths infected with Pseudomonas aeruginosa strain PAO1 (wild type) [1]
Dose: 2.5 μM (in combination with 0.001 mg/mL ciprofloxacin or 0.0025 mg/mL tobramycin) Route of Administration:Intracavitary injection; single administration Experimental Experimental Results:When combined with tobramycin, the larval survival rate on day 5 exceeded 70%, while the survival rate was 40% when tobramycin was used alone. When combined with ciprofloxacin, the larval survival rate on day 5 was close to 80%, which was statistically significantly higher than that when ciprofloxacin was used alone. Animal/Disease Models:Wax moths infected with Pseudomonas aeruginosa PAO1-ΔwspF strain (high biofilm mutant) [1] Doses: 0.156 μM (in combination with ciprofloxacin 0.001 mg/mL or tobramycin 0.0025 mg/mL) Route of Administration:Intraperitoneal injection; single administration Experimental Results:When combined with tobramycin or ciprofloxacin, the larval survival rate on day 5 was significantly higher than when the corresponding antibiotics were used alone. |
| References |
| Molecular Formula |
C19H21IN2O3S
|
|---|---|
| Molecular Weight |
484.35
|
| Appearance |
Typically exists as solids at room temperature
|
| SMILES |
C[N+]1=C(SC2=CC=CC=C21)/C=C/C(N(C=C3O)CCCOC)=CC3=O.[I-]
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.0646 mL | 10.3231 mL | 20.6462 mL | |
| 5 mM | 0.4129 mL | 2.0646 mL | 4.1292 mL | |
| 10 mM | 0.2065 mL | 1.0323 mL | 2.0646 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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