| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| ln Vitro |
AlDeSense AM (1.5–2 μM; 30 min) selectively labels human chronic myeloid leukemia K562 cells with active ALDH1A1, which correspond to the CD34+/CD38−/CD133+ leukemia stem cell population [1]. AlDeSense AM (2 μM; 1 h) detected increased ALDH1A1 activity in MDA-MB-231 breast cancer mammary globules (rich in cancer stem cells), and the fluorescence signal gradually decreased as the mammary globules differentiated over 36 hours [1]. In cancer stem cell-rich B16F0 e-CSCs, the fluorescence signal of AlDeSense AM (2 μM; 1 h) was 11.3 times higher than in non-cancer stem cell B16F0 cells, confirming increased ALDH1A1 activity in e-CSCs [1].
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| ln Vivo |
AlDeSense AM (15 μM; lung perfusion; single dose) selectively detected increased ALDH1A1 activity in e-CSC-derived lung metastases in C57BL/6J mice, and the resulting fluorescence signal was significantly higher than that in non-CSC-derived metastases [1].
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| Cell Assay |
Immunofluorescence [1]
Cell Types: K562 human chronic myeloid leukemia cells Tested Concentrations: 1.5 μM (flow cytometry staining); 2 μM (confocal imaging staining) Incubation Duration: 30 minutes at room temperature (confocal imaging staining) Experimental Results: A group of bright fluorescent cells with CD34+/CD38-/CD133+ leukemia stem cell characteristics were identified. A small number of highly fluorescent cells were shown by confocal imaging. The fluorescence signal disappeared completely after ALDH1A1 siRNA knockdown. Immunofluorescence [1] Cell Types: MDA-MB-231 breast cancer mammary globules (rich in CSCs) and differentiating mammary globules Tested Concentrations: 2 μM Incubation Duration: 1 hour at room temperature (incubation was performed at 1, 6, 12, 24 and 36 hours during differentiation) Experimental Results: Compared with the control stained mammary globules, the fluorescence intensity of freshly isolated mammary globules increased by 3 times. During the 36-hour differentiation process, the fluorescence signal gradually weakened. Immunofluorescence [1] Cell Types: B16F0 mouse melanoma e-CSCs (CSCs enriched by spiral pattern hydrogel culture) and non-CSCs (standard culture) Tested Concentrations: 2 μM Incubation Duration: 1 hour at room temperature Experimental Results: The fluorescence intensity of e-CSCs was 11.3 times higher than that of non-CSCs. The fluorescence intensity of e-CSCs was 9.0 times higher than that of e-CSCs stained with the control reagent. Co-localization with the melanoma stem cell marker CD271 in e-CSCs. |
| Animal Protocol |
Animal/Disease Models:C57BL/6J (female, 6 to 8 weeks old) [1]
Doses: 15 μM Route of Administration: Lung perfusion; single dose Experimental Results: The average fluorescence signal in the lungs with e-CSC metastases was ~1×1011 au. The fluorescence intensity of the e-CSC metastases was significantly higher than that of the lungs without CSC metastases, which had an average fluorescence signal of ~7×1010 au. |
| References |
| Molecular Formula |
C24H16F2O6
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|---|---|
| Molecular Weight |
438.38
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| CAS # |
2243148-62-9
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| Appearance |
Typically exists as solids at room temperature
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| SMILES |
CC(OCOC1=C(C=C2C(OC3=CC(C(F)=CC3=C2C4=CC=C(C=C4C)C=O)=O)=C1)F)=O
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.2811 mL | 11.4056 mL | 22.8113 mL | |
| 5 mM | 0.4562 mL | 2.2811 mL | 4.5623 mL | |
| 10 mM | 0.2281 mL | 1.1406 mL | 2.2811 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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