| Size | Price | Stock | Qty |
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| ln Vitro |
AA-BR-157 (0-30 μM; 24 hours) (compound 17) effectively degraded MT2A overexpressed in MDA-MB-231 cells, with a half-maximal lethal concentration (DC50) of 200 nM and a maximum lethal concentration (Dmax) of 90% [1]. AA-BR-157 (0-10 μM; 24 hours) inhibited the migration of MDA-MB-231 and U-87 MG cells, as well as the invasion of MDA-MB-231 cells [1]. AA-BR-157 (10 μM; 12-24 hours) increased the zinc level in MDA-MB-231 cells [1]. AA-BR-157 (0-10 μM; 24 hours) downregulated the expression of DIAPH3 in MDA-MB-231 cells [1]. AA-BR-157 (10 μM; 24 h) induced MDA-MB-231 cells to produce a non-polarized, non-migrating cytoskeleton morphology [1].
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| Cell Assay |
Western Blot Analysis [1]
Cell Types: MDA-MB-231 (MT2A OE) cells stably overexpressing MT2A-V5 Tested Concentrations: 0, 0.01, 0.03, 0.1, 0.3, 1, 3, 10, 30 μM (24-hour incubation); 10 μM (time-course analysis) Incubation Duration: 24 hours (dose-dependent assay); different times (time-course analysis) Experimental Results: MT2A levels were induced to decrease in a dose-dependent manner, with a half-maximal degradation concentration (DC50) of 200 nM and a maximum degradation rate (Dₘₐₓ) of 90%, starting at approximately 1 μM. No protein recovery was observed at high concentrations, indicating the absence of the Hooke effect. The half-maximum degradation time (D50) was 14.6 hours, and the maximum degradation rate was reached after 24 hours. Cell migration assay [1] Cell Types: MDA-MB-231 cells, U-87 MG cells Tested Concentrations: 0, 1, 3, 10 μM Incubation Duration: 24 hours Experimental Results: The concentration-dependent decrease in cell migration was observed. At 10 μM, the migration rates of both MDA-MB-231 and U-87 MG cells decreased by more than 60%, consistent with the effect of MT siRNA knockdown. At 10 μM, the invasion of MDA-MB-231 cells through Matrigel was inhibited. No cell death was detected by WST-1 assay, confirming that this effect was not caused by cytotoxicity. Western blot analysis [1] Cell Types: MDA-MB-231 cells Tested Concentrations: 0, 3, 10 μM Incubation Duration: 24 hours Experimental Results: Whole proteomics analysis showed that DIAPH3 expression was downregulated in the AA-BR-157/DMSO treatment group, with a ratio of 0.31, consistent with the knockdown effect of MT siRNA. Western blot detection confirmed that DIAPH3 level decreased by about 50% after treatment with 10 μM AA-BR-157, similar to the effect of MT siRNA. |
| References |
| Molecular Formula |
C56H70N8O11S2
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|---|---|
| Molecular Weight |
1095.33
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| Appearance |
White to off-white solid
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| SMILES |
O=C(NCC1=NC2=CC(C3=CC=CC(C(N4CCN(C(C=C)=O)CC4)=O)=C3)=CC=C2S1)CCOCCOCCOCCOCCC(N[C@@H](C(C)(C)C)C(N5[C@H](C(NCC6=CC=C(C7=C(C)N=CS7)C=C6)=O)C[C@@H](O)C5)=O)=O
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~91.30 mM; with sonication)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.9130 mL | 4.5648 mL | 9.1297 mL | |
| 5 mM | 0.1826 mL | 0.9130 mL | 1.8259 mL | |
| 10 mM | 0.0913 mL | 0.4565 mL | 0.9130 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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