| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
| ln Vitro |
(Rac)-AAA (5-10 μM; 12 h) can reverse 20-HETE-induced decrease in GPR75 expression and increase basal GPR75 expression in PC-3 human prostate cancer cells [1]. (Rac)-AAA (5-10 μM; 2 h) can reduce 20-HETE-induced phosphorylation of EGFR, AKT and NF-κB and increase basal p38 phosphorylation in PC-3 human prostate cancer cells [1]. (Rac)-AAA (5-10 μM; 24 h) can reverse 20-HETE-induced epithelial-mesenchymal transition in PC-3 human prostate cancer cells by restoring E-cadherin expression and reducing or eliminating vimentin expression [1]. (Rac)-AAA (5-10 μM; 24 h) can reduce or eliminate 20-HETE-induced MMP-2 activity in conditioned medium of PC-3 human prostate cancer cells [1]. (Rac)-AAA (5 μM; 21 days) can eliminate 5,14-HEDGE-induced increase in non-anchor-dependent colony formation in PC-3 human prostate cancer cells [1].
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| Cell Assay |
Western Blot Analysis [1]
Cell Types: PC-3 human prostate cancer cells Tested Concentrations: 5 μM (co-incubated with 20-HETE); 10 μM (co-incubated with 20-HETE); 10 μM (incubated alone) Incubation Duration: 2 hours (all conditions) Experimental Results: Compared with 20-HETE alone, 20-HETE induced EGFR phosphorylation decreased by 44% (5 μM) and 68% (10 μM), respectively. Compared with 20-HETE alone, 20-HETE induced AKT phosphorylation decreased by 40% (5 μM) and 58% (10 μM), respectively. Compared with 20-HETE alone, 20-HETE induced a 66% reduction in NF-κB phosphorylation levels (at both 5 μM and 10 μM concentrations). Baseline p38 phosphorylation levels were increased by 248% compared with the control group. Western Blot Analysis [1] Cell Types: PC-3 human prostate cancer cells Tested Concentrations: 5 μM (co-incubated with 20-HETE); 10 μM (co-incubated with 20-HETE) Incubation Duration: 24 hours (all conditions) Experimental Results: Reversed 40% downregulation of E-cadherin induced by 20-HETE. Compared with 20-HETE alone, the 150% upregulation of vimentin induced by 20-HETE was reduced by 75% at 5 μM concentration, while this upregulation was completely inhibited at 10 μM concentration. Cell migration assay [1] Cell Types: PC-3 human prostate cancer cells Tested Concentrations: 5 μM (co-incubated with 20-HETE) Incubation Duration: 16 hours Experimental Results: Compared with 20-HETE alone, the wound healing rate induced by 20-HETE decreased from 48.9% to 36.0%. |
| References |
| Molecular Formula |
C24H39NNA2O6
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|---|---|
| Molecular Weight |
483.55
|
| CAS # |
3055022-23-3
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| Appearance |
White to off-white solid
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| SMILES |
O=C(C(NC(CCCC/C=C\CCCCCCC/C=C\CCCCO)=O)CC(O[Na])=O)O[Na]
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.0680 mL | 10.3402 mL | 20.6804 mL | |
| 5 mM | 0.4136 mL | 2.0680 mL | 4.1361 mL | |
| 10 mM | 0.2068 mL | 1.0340 mL | 2.0680 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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