| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| ln Vitro |
MRS2690 (0.001-10 μM) disodium induced concentration-dependent contraction of isolated coronary arteries in porcines under basal tension (log EC50 = 6.30 M) [1]. MRS2690 (0.001-10 μM) disodium showed enhanced concentration-dependent contractile response (Rmax = 1.77 g) in porcine coronary arteries pretreated with Forskolin and U46619, which could be blocked by the P2Y14 antagonist PPTN but not by the P2Y6 antagonist MRS2578 [1]. MRS2690 (10 μM) disodium significantly reduced Forskolin-induced VASP phosphorylation (an indicator of cAMP levels) in porcine coronary arteries [1]. MRS2690 (0.0001-1000 nM) disodium induced intracellular calcium mobilization in RBL-2H3 cells in a concentration-dependent manner, with an EC50 of 538 nM[2]. MRS2690 (0.0001-100 nM) disodium stimulated [35S]GTPγS binding in the RBL-2H3 cell membrane in a concentration-dependent manner, with an EC50 of 8.1 nM[2]. MRS2690 (1 μM) disodium activated p38 mitogen-activated protein kinase (MAPK) in RBL-2H3 cells[2]. MRS2690 alone could not induce β-hexosaminease (HEX) release, but in RBL-2H3 cells pretreated with anti-DNP-BSA antibody, it enhanced antigen (DNP-BSA)-induced HEX release in a concentration-dependent manner, with an EC50 of 103 nM[2]. MRS2690 (1 μM, 50 min) disodium significantly enhanced the release of NP-BSA and C3a-induced β-hexosaminease (β-Hex) in LAD2 cells[3]. MRS2690 (10-1000 nM) disodium significantly induced platelet-dependent neutrophil chemotaxis to macrophage-derived chemokines (MDC, CCL22) in a concentration-dependent manner[4].
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|---|---|
| References |
| Molecular Formula |
C15H22N2NA2O16P2S
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|---|---|
| Molecular Weight |
626.33
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| Related CAS # |
MRS2690
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| Appearance |
Typically exists as solids at room temperature
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.5966 mL | 7.9830 mL | 15.9660 mL | |
| 5 mM | 0.3193 mL | 1.5966 mL | 3.1932 mL | |
| 10 mM | 0.1597 mL | 0.7983 mL | 1.5966 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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