| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| ln Vitro |
TBE56 (100 nM, 3 h) significantly reduced BACH1 protein levels in HaCaT cells, with an EC50 of 44 nM [1]. TBE56 (100 nM, 16 h) induced HMOX1 mRNA expression in HaCaT cells [1]. TBE56 (0.05-5 μM, 5-16 h) induced HMOX1 mRNA expression in A549, H1299, MDA-MB-231, and MDA-MB-468 cells, promoted BACH1 degradation, and reduced nuclear BACH1 levels in a concentration-dependent manner [1]. TBE56 (100 nM, 6 h) reduced the migration and invasion abilities of wild-type MDA-MB-231 cells [1]. TBE56 (110 nM, 3 h pretreatment) significantly reduced the accumulation of Fe2+ and ROS generation in Prominin-2-overexpressed BMSCs[2]. TBE56 (110 nM, 3 h pretreatment) significantly increased the GSH/GSSG ratio of Prominin-2-overexpressed BMSCs and reduced MDA content[2]. TBE56 (110 nM, 16 h pretreatment) significantly improved the activity of degenerative NPCs and reduced LDH release[2].
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| ln Vivo |
TBE56 (in the case of bone marrow mesenchymal stem cells (BMSCs) overexpressing Prominin-2, 1×10⁴ cells/μL, 10 μL/support, intradiscal injection, once a week for 2 months) significantly improved the rescue of the SD intervertebral disc degeneration (IVDD) model [1].
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| Cell Assay |
Western Blot Analysis[1]
Cell Types: HaCaT cells Tested Concentrations: 100 nM Incubation Duration: 3 h Experimental Results: Reduced BACH1 protein levels with an EC50 of 44 nM in HaCaT cells. |
| Animal Protocol |
Animal/Disease Models: Four-week-old male Sprague-Dawley (SD) rats (160–180 g): A needle was punctured into the center of lumbar IVD segments to induce disc degeneration, and SD rats’ forelimbs were amputated with the food trough raised post-surgery for 1-week recovery, establishing a puncture-induced SD rat IVDD model[1]
Doses: Pretreatment of Prominin-2-overexpressed BMSCs, 1×104 cells/μL, 10 μL per rat, Route of Administration: Intradiscal injection, every 2 weeks for 2 months Experimental Results: Achieved enhanced retention of transplanted Prominin-2-overexpressed BMSCs in degenerated IVDs of SD rats. Showed no significant changes in body weight. Reduced the histological scores of IVD degeneration in SD rats. Appeared elevated disc height index (DHI) percentage. Showed greater amelioration of IVD degeneration with downregulated protein levels of MMP-9, ADAMTS5, and MMP-13 in degenerated IVD tissues of SD rats. Achieved improved histological morphology of IVDs. Reduced lipid peroxidation and oxidative stress in degenerated IVD microenvironment. |
| References |
| Molecular Formula |
C37H43N5O6S
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|---|---|
| Molecular Weight |
685.83
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| Appearance |
Typically exists as solids at room temperature
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.4581 mL | 7.2904 mL | 14.5809 mL | |
| 5 mM | 0.2916 mL | 1.4581 mL | 2.9162 mL | |
| 10 mM | 0.1458 mL | 0.7290 mL | 1.4581 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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