| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| ln Vitro |
Carbonic anhydrase-IN-2 (Compound 20) (184-3060 nM, 18-20 h) can inhibit the proliferation of various vancomycin-resistant enterococcal (VRE) strains, including E. faecium NR-31909 and E. faecalis NR-31971, with a MIC50 of 184 nM and a MIC90 of 3060 nM[1]. Carbonic anhydrase-IN-2 can strongly inhibit α-carbonic anhydrase (Efα-CA) (Ki value of 31.6 nM) and γ-carbonic anhydrase (Efγ-CA) (Ki value of 62.3 nM) in E. faecium[1]. Carbonic anhydrase-IN-2 (24 h) has antibacterial activity against E. faecium NR-31909[1]. Carbonic anhydrase-IN-2 (18-20 h) showed no inhibitory activity against 12 intestinal symbiotic strains, including Lactobacillus, Bacteroides and Bifidobacterium, with a MIC greater than 784 μM[1]. Carbonic anhydrase-IN-2 (0-128 μg/mL, 24 h) showed no significant cytotoxicity in African green monkey kidney cells and maintained 95-100% cell viability[1].
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|---|---|
| ln Vivo |
Carbonic anHydrase-IN-2 (Compound 20) (20 mg/kg, interface, daily, for 3 consecutive days of infection, monitoring from 1 hour after the first infection) significantly improved the asphyxia rate in BALB/c patients infected with Enterococcus faecium R-31909 with acute peritonitis [1].
|
| Cell Assay |
Cell Viability Assay[1]
Cell Types: Vero cells Tested Concentrations: 0, 4, 8, 16, 32, 64 nd 128 μg/mL Incubation Duration: 24 h Experimental Results: Maintained cell viability at 95-100%. |
| Animal Protocol |
Animal/Disease Models: E. faecium NR-31909 septicemic peritonitis[1]
Doses: 20 mg/kg Route of Administration: Orally administration, daily for 3 days, starting 1 h post-infection Experimental Results: Reduced NR-31909 burden by 96.8% in kidney, 98.7% in liver, and 96.8% in spleen. |
| References |
| Molecular Formula |
C12H14N4O3S2
|
|---|---|
| Molecular Weight |
326.39
|
| Appearance |
Typically exists as solids at room temperature
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.0638 mL | 15.3191 mL | 30.6382 mL | |
| 5 mM | 0.6128 mL | 3.0638 mL | 6.1276 mL | |
| 10 mM | 0.3064 mL | 1.5319 mL | 3.0638 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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