| Size | Price | Stock | Qty |
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| 1mg |
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| Other Sizes |
| Targets |
Dicytidine 5'-triphosphate trisodium targets cellular transport processes and gene expression pathways. It inhibits the intracellular accumulation of trans-resveratrol and trans-piceid, suggesting interference with cellular uptake mechanisms or efflux transporters. The compound also induces the expression of CCR2 (C-C motif chemokine receptor 2), a key receptor involved in monocyte/macrophage recruitment and inflammation. Additionally, it upregulates the expression of PAL1 (phenylalanine ammonia-lyase) and 4CL1 (4-coumarate-CoA ligase), enzymes involved in phenylpropanoid metabolism.
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| ln Vitro |
Dicytidine 5'-triphosphate trisodium exhibits several in vitro biological activities. It inhibits the intracellular accumulation of trans-resveratrol and trans-piceid in cells, suggesting interference with cellular uptake or transport mechanisms. The compound induces the expression of CCR2, a chemokine receptor involved in monocyte recruitment and inflammation, making it a potential research tool for studying inflammatory pathways. Additionally, it upregulates the expression of PAL1 and 4CL1, which are key enzymes in the phenylpropanoid biosynthetic pathway. The compound is water-soluble (H2O : ~10 mg/mL, ~12.91 mM), which facilitates its use in cellular assays.
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| ln Vivo |
No specific in vivo activity data for Dicytidine 5'-triphosphate trisodium is available. As a dinucleotide polyphosphate, it has potential applications in studying nucleotide signaling, inflammation, and metabolic regulation. The induction of CCR2 expression suggests potential relevance in models of inflammation, monocyte recruitment, and immune response. Upregulation of PAL1 and 4CL1 indicates potential effects on phenylpropanoid metabolism, which is relevant in plant biology but may also have implications in mammalian systems through metabolic pathways.
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| Enzyme Assay |
Not applicable. Dicytidine 5'-triphosphate trisodium is a dinucleotide polyphosphate and is not a standard enzyme/receptor inhibitor in the classical pharmacological sense. Its biological activity can be assessed by measuring its effects on cellular accumulation of compounds (e.g., trans-resveratrol) using LC-MS/MS quantification. CCR2 expression can be measured by RT-qPCR or Western blotting of treated cells. PAL1 and 4CL1 expression can be similarly quantified. The compound is soluble in H2O and can be used in biochemical assays directly.
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| Cell Assay |
For cellular transport assays, cells (e.g., Caco-2, HepG2, or HEK293) are seeded in 6-well plates and cultured overnight. Dicytidine 5'-triphosphate trisodium (1-100 uM) is added for 30-60 min, followed by addition of trans-resveratrol (10-50 uM). After 1-4 h incubation, cells are harvested, lysed, and intracellular resveratrol concentration is quantified by LC-MS/MS. For CCR2 expression, cells are treated with Dicytidine 5'-triphosphate trisodium for 6-24 h, and CCR2 mRNA is measured by RT-qPCR. Cell viability is assessed by MTT assay in parallel to ensure that observed effects are not due to cytotoxicity.
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| Animal Protocol |
No specific in vivo animal protocol for Dicytidine 5'-triphosphate trisodium is available. For studying CCR2 induction in vivo, female BALB/c mice (6-8 wk, n=5/group) are administered Dicytidine 5'-triphosphate trisodium intraperitoneally (10-50 mg/kg) or intravenously. After 4-24 h, blood and tissues (liver, spleen, lung) are harvested. CCR2 expression is measured by RT-qPCR and immunohistochemistry. For inflammation models, the compound could be administered prior to LPS challenge, and inflammatory cytokine levels (TNF-alpha, IL-6, MCP-1) measured by ELISA. This protocol is hypothetical; no specific data for the compound is available.
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| ADME/Pharmacokinetics |
No specific PK data for Dicytidine 5'-triphosphate trisodium is available. As a dinucleotide polyphosphate (MW 774.30, highly negatively charged), it is unlikely to cross cell membranes passively. It is water-soluble and would require injection (IV or IP) for in vivo studies. Following IV administration, it would be rapidly cleared from circulation by renal filtration and possibly by cellular uptake mechanisms. It may be dephosphorylated by ectonucleotidases to form smaller nucleotides. The trisodium salt form enhances water solubility (H2O : ~10 mg/mL). Half-life in vivo is expected to be short (<1 hour).
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| Toxicity/Toxicokinetics |
For Dicytidine 5'-triphosphate trisodium, storage conditions: powder at -20degC for 3 years, 4degC for 2 years; in solvent at -80degC for 6 months, -20degC for 1 month. Store in a sealed and protected environment, avoid exposure to moisture. Shipping at room temperature (stable for a few days). The compound is for research use only, not for human use. Standard safety precautions for handling nucleotides apply: use PPE (gloves, lab coat, goggles), work in a fume hood, avoid inhalation and skin contact.
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| References | |
| Additional Infomation |
Dicytidine 5'-triphosphate trisodium (CAS# 1629163-10-5) is a research-grade dinucleotide polyphosphate used as a biochemical tool to study trans-resveratrol transport, CCR2 expression, and phenylpropanoid metabolism. It is not an FDA-approved drug. For research use only, not for diagnostic or therapeutic applications. Purity: 99.94%.
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| Molecular Formula |
C18H24N6NA3O18P3
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|---|---|
| Molecular Weight |
774.30
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| CAS # |
1629163-10-5
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| Related CAS # |
Dicytidine 5′-triphosphate
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| Appearance |
Solid powder
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| SMILES |
NC(C=CN1[C@H]2[C@H](O)[C@H](O)[C@@H](COP(OP(OP(OC[C@H]3O[C@@H](N4C=CC(N)=NC4=O)[C@H](O)[C@@H]3O)(O[Na])=O)(O[Na])=O)(O[Na])=O)O2)=NC1=O
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| Synonyms |
Cp3C trisodium
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~10 mg/mL (~12.91 mM; with sonication)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.2915 mL | 6.4574 mL | 12.9149 mL | |
| 5 mM | 0.2583 mL | 1.2915 mL | 2.5830 mL | |
| 10 mM | 0.1291 mL | 0.6457 mL | 1.2915 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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