| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg | |||
| Other Sizes |
| ln Vitro |
Autophagy inducer 7 (72 h) inhibits the growth of human lung adenocarcinoma cell lines with IC50s of 1.95 μM (HOP-62), 4.72 μM (A549), and 3.05 μM (H1299)[1]. Autophagy inducer 7 (0-10 μM, 24 h) induced apoptosis in HOP-62, A549, and H1299 cells, but not as significantly as Sulindac sulfide and Staurosporine [1]. Autophagy inducer 7 (0-7.5 μM, 24 h) inhibits DNA synthesis in HOP-62, A549, and H1299 cells and increases the percentage of cells in the G0-G1 phase of the cell cycle [1]. Autophagy inducer 7 (0-7.5 μM, 24 h) induces autophagy and increases autophagic flux by inhibiting Akt/mTOR/p70S6k signaling in A549 cells [1].
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| Cell Assay |
Apoptosis Analysis[1]
Cell Types: HOP-62, A549, H1299 cells Tested Tested Concentrations: 2.5, 5, 7.5, 10 μM Incubation Duration: 24 h Experimental Results: Induced caspase3/7 activation dose-dependently. Induced cleaved PARP expression at 10 μM. Increased Annexin-V surface staining and PI labeling. Cell Autophagy Assay[1] Cell Types: HOP-62, A549, H1299 cells Tested Tested Concentrations: 2.5, 5, 7.5, 10 μM Incubation Duration: 24 h Experimental Results: Induced dose- and time-dependent cleavage of cytosolic LC3-I to autophagosome-associated LC3-II. Decreased p62 expression. Displayed a striking accumulation of vesicle-like structures within the cytoplasm. Increased both the number and size of acidic vesicles within the cytoplasm. |
| References |
| Molecular Formula |
C24H27FN2OS
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|---|---|
| Molecular Weight |
410.55
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| CAS # |
944159-20-0
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| Appearance |
Solid powder
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~121.79 mM; with ultrasonication)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4358 mL | 12.1788 mL | 24.3576 mL | |
| 5 mM | 0.4872 mL | 2.4358 mL | 4.8715 mL | |
| 10 mM | 0.2436 mL | 1.2179 mL | 2.4358 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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