| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| ln Vitro |
ASM-IN-3 (40 μM, 0.5-4 h) can effectively penetrate the hCMEC/D3 cell membrane[1]. ASM-IN-3 (0.098-12.5 μM) is safe for NIH3T3 cells (IC50: 161 μM)[1].
|
|---|---|
| ln Vivo |
ASM-IN-3 (48 mg/kg, intraperitoneal injection) can well penetrate the blood-brain barrier of SD rats because its content in brain tissue is much higher than that in plasma[1]. ASM-IN-3 (6-24 mg/kg, intravenous injection, twice a day for 2 weeks) showed good antidepressant-like effects in Reserpine-induced depressed rats by reducing ASM activity in the hippocampus and restoring neurogenesis[1].
|
| Animal Protocol |
Animal/Disease Models: Reserpine-induced depressed SD rats (6−8 weeks, 200 g, female, were injected intraperitoneally with Reserpine (4 mg/kg) daily)[1]
Doses: 6, 12, 24 mg/kg Route of Administration: Intraperitoneal injection (i.p.), twice a day for 2 weeks Experimental Results: Improved sugar consumption in a dose dependent manner after 2 days of tap water drinking and following 20 h of water depletion and fasting. Decreased the total time in center of rats, and increased times passing the grid and frequency of grooming in the open-field test. Demonstrated a reduction of the cortical and hippocampal ASM activity by approximately 27% and 33%, respectively, compared to the model group at a dose of 24 mg/kg. Prevented the inhibitory effect of Reserpine on neurogenesis and restored neurogenesis. |
| References |
| Molecular Formula |
C17H13CLN2O4
|
|---|---|
| Molecular Weight |
344.75
|
| CAS # |
2342576-75-2
|
| Appearance |
Typically exists as solids at room temperature
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9007 mL | 14.5033 mL | 29.0065 mL | |
| 5 mM | 0.5801 mL | 2.9007 mL | 5.8013 mL | |
| 10 mM | 0.2901 mL | 1.4503 mL | 2.9007 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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