| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
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| ln Vitro |
ATF4-IN-1 inhibits the expression of ATF4 in HEK-293T cells in a dose-dependent manner with an IC50 of 32.43 nM[1]. ATF4-IN-1 (0-1000 nM, 3 h) inhibits the protein expression of ATF4 in HEK-293T cells[1]. ATF4-IN-1 (200 nM, 3 h) inhibits the transcription of ATF4 mRNA in HeLa cells[1]. ATF4-IN-1 activates eIF2B with an EC50 of 5.844 nM in HEK-293T cells[1]. ATF4-IN-1 inhibits the proliferation of HEK-293T cells with an IC50 of 96 μM.
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|---|---|
| Cell Assay |
Western Blot Analysis[1]
Cell Types: HEK-293 T Tested Tested Concentrations: 0, 1, 10, 100, 500, 1000 nM Incubation Duration: 3 h Experimental Results: Inhibited ATF4 expression. Real Time qPCR[1] Cell Types: HeLa Tested Tested Concentrations: 200 nM Incubation Duration: 3 h Experimental Results: Inhibited mRNA transcription of ATF4. |
| References |
| Molecular Formula |
C24H21CL2N3O4
|
|---|---|
| Molecular Weight |
486.35
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| CAS # |
2991057-76-0
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| Appearance |
White to off-white solid powder
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~8.33 mg/mL (~17.13 mM; with ultrasonication (<60°C))
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| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.0561 mL | 10.2807 mL | 20.5613 mL | |
| 5 mM | 0.4112 mL | 2.0561 mL | 4.1123 mL | |
| 10 mM | 0.2056 mL | 1.0281 mL | 2.0561 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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