| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
Human Endogenous Metabolite
Not applicable. As a nucleotide, 3'-UMP disodium is not a drug with a specific pharmacological target. It is an endogenous metabolite used as an RNA component and an analytical standard. It may be involved in the sodium-potassium pump's activity and the de novo pyrimidine synthesis pathway. |
|---|---|
| ln Vitro |
3'-UMP disodium is a nucleotide that serves as a component of ribonucleic acid (RNA). It has been shown to be converted to uracil in the rat jejunum. As a biochemical reagent, its primary activity is in facilitating nucleic acid synthesis and serving as a substrate in metabolic pathways. It is recognized for its ability to enhance RNA synthesis, making it an essential component in molecular biology and genetic research.
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| ln Vivo |
A study in rat jejunum demonstrated that 3'-UMP is transported and metabolized, being converted to uracil. This indicates its bioavailability and metabolic processing in the gastrointestinal tract. However, no specific pharmacological in vivo data for therapeutic applications is available, as it is a biochemical reagent rather than an administered drug.
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| Enzyme Assay |
Not applicable. 3'-UMP disodium is an analytical standard and biochemical reagent, not typically used in non-cellular enzyme activity assays as a test compound. For nucleotide quantification, a standard protocol involves dissolving the compound in water or buffer to prepare calibration standards of known concentrations (e.g., 0.5, 1, 5, 10, 50, 100 uM), which are then analyzed by LC-MS/MS to generate standard curves for bioanalysis.
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| Cell Assay |
Not applicable. 3'-UMP disodium is a nucleotide reagent used for RNA synthesis and biochemical research, not as a modulator in live cell assays. In studies of nucleotide metabolism, the compound can be added to cell culture media (e.g., 50-500 uM) for studying pyrimidine metabolism or as a supplement for nucleotide salvage pathways. RNA synthesis enhancement may be measured by incorporation of radiolabeled precursors.
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| Animal Protocol |
Not applicable. 3'-UMP disodium is not administered to animals as a test drug. In a rat jejunum transport study, 3'-UMP was introduced into an in vitro gut sac preparation to investigate its uptake and metabolism, where it was found to be dephosphorylated and the uridine further metabolized to uracil. This is a study of nucleotide metabolism rather than a therapeutic efficacy study.
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| ADME/Pharmacokinetics |
Not applicable. Pharmacokinetic studies are not performed on 3'-UMP disodium as it is not a drug candidate. As an endogenous nucleotide, if administered exogenously, it would be rapidly dephosphorylated and metabolized, with the resulting uridine and uracil entering the pyrimidine salvage pathways. The compound is an analytical standard, not a drug for systemic administration.
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| Toxicity/Toxicokinetics |
Not applicable. 3'-UMP disodium is an endogenous nucleotide and biochemical reagent. Its sodium salt form is considered safe for laboratory handling under standard practices. Toxicological studies are not required for this research reagent. For research use only, not for human or veterinary consumption.
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| References | |
| Additional Infomation |
3'-Ump(2-) is a nucleoside 3'-phosphate(2) obtained by deprotonation of the phosphoryl hydroxyl group of uridine 3'-monophosphate (UMP). It is the predominant form at physiological pH. It is a human metabolite and the conjugate base of 3'-UMP.
3'-UMP disodium (Uridine 3'-monophosphate disodium salt) is supplied as a research-grade nucleotide with molecular formula C9H11N2Na2O9P and molecular weight 368.14. Purity ≥99%. Soluble in water (15 mg/mL) and DMSO. Store at -20degC for long-term stability. It is used in nucleic acid research, RNA synthesis studies, and as a standard for HPLC or LC-MS/MS-based metabolomics analyses. |
| Molecular Formula |
C9H11N2NA2O9P
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|---|---|
| Molecular Weight |
368.14
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| Exact Mass |
322.02
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| CAS # |
35170-03-7
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| PubChem CID |
23421206
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| Appearance |
Typically exists as solids at room temperature
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| Density |
1.88 g/cm3
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| Melting Point |
>215°C (dec.)
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| LogP |
0
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
9
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
21
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| Complexity |
506
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| Defined Atom Stereocenter Count |
4
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| SMILES |
C1=CN([C@H]2[C@@H]([C@@H]([C@@H](CO)O2)OP(=O)(O)[O-])O)C(=O)N=C1[O-].[Na+].[Na+]
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| InChi Key |
FOGRQMPFHUHIGU-XVFCMESISA-L
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| InChi Code |
InChI=1S/C9H13N2O9P/c12-3-4-7(20-21(16,17)18)6(14)8(19-4)11-2-1-5(13)10-9(11)15/h1-2,4,6-8,12,14H,3H2,(H,10,13,15)(H2,16,17,18)/p-2/t4-,6-,7-,8-/m1/s1
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| Chemical Name |
[(2R,3S,4R,5R)-5-(2,4-dioxopyrimidin-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] phosphate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7164 mL | 13.5818 mL | 27.1636 mL | |
| 5 mM | 0.5433 mL | 2.7164 mL | 5.4327 mL | |
| 10 mM | 0.2716 mL | 1.3582 mL | 2.7164 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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