| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg |
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| Other Sizes |
| Targets |
Not applicable. 4-Hydroxy alprazolam is a drug metabolite. The parent drug, alprazolam, acts as a positive allosteric modulator (PAM) of the GABA-A receptor, enhancing the inhibitory effects of GABA. The 4-hydroxy metabolite is generally considered to have significantly reduced activity at the GABA-A receptor compared to the parent compound. Its primary role is as a biomarker of alprazolam exposure.
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|---|---|
| ln Vitro |
No relevant in vitro pharmacological activity data is available for 4-Hydroxy alprazolam itself. It is an oxidative metabolite formed primarily by CYP3A4. As a research standard, it is used in analytical chemistry to study the metabolic clearance of alprazolam. Information about its own potency or efficacy is not needed for this application.
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| ln Vivo |
No specific in vivo data is applicable, as 4-Hydroxy alprazolam is not administered as a therapeutic agent. It is produced endogenously in the liver following the administration of alprazolam. Its levels in plasma or urine are used to determine the pharmacokinetics of alprazolam and to confirm patient compliance or toxicity.
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| Enzyme Assay |
Not applicable. 4-Hydroxy alprazolam is an analytical reference standard. A typical protocol for its use in LC-MS/MS: A calibration curve is generated by spiking known concentrations of 4-Hydroxy alprazolam into a blank biological matrix (e.g., serum, plasma, or urine). A stable isotope-labeled internal standard, such as 4-Hydroxy alprazolam-d4, is added to all samples. After sample clean-up by protein precipitation or SPE, the samples are analyzed by LC-MS/MS. The concentration in unknown samples is calculated from the calibration curve.
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| Cell Assay |
Not applicable. 4-Hydroxy alprazolam is not a drug and is not used as a research tool in cell-based assays. It can be used as an analytical standard to measure the metabolic capacity of cultured hepatocytes or microsomes. For example, human liver microsomes are incubated with alprazolam, and the resulting 4-Hydroxy alprazolam is quantified using a standard curve prepared from this material to determine the rate of metabolism.
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| Animal Protocol |
4-Hydroxy alprazolam is a primary metabolite of alprazolam. It is not administered directly to animals. Instead, the pharmacokinetics of alprazolam are assessed in animals (e.g., Sprague Dawley rats) by administering the parent drug. The resulting blood and urine samples are then analyzed for both parent drug and its metabolite, 4-Hydroxy alprazolam. This helps determine the compound's metabolic stability, clearance, and oral bioavailability.
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| ADME/Pharmacokinetics |
Metabolism / Metabolites
4-Hydroxyalprazolam is a known metabolite of alprazolam in the human body. No specific PK data was found for 4-Hydroxy alprazolam. As a drug metabolite, its pharmacokinetic profile is determined by the PK of the parent drug, alprazolam. Alprazolam has a peak plasma concentration (Cmax) around 1-2 hours and a half-life (t1/2) of approximately 11-12 hours. 4-Hydroxy alprazolam appears in plasma shortly after alprazolam administration and is eventually conjugated and excreted in urine. |
| Toxicity/Toxicokinetics |
No specific toxicity data was found for 4-Hydroxy alprazolam. It is considered a major metabolite of alprazolam and is typically not associated with significant toxicity or pharmacological activity. However, as with any analytical standard, it should be handled with care and proper laboratory precautions.
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| References | |
| Additional Infomation |
4-Hydroxyalprazolam is a triazolamide benzodiazepine. Alprazolam metabolites
4-Hydroxy alprazolam (CAS: 30896-57-2) has a molecular formula of C17H13ClN4O and a molecular weight of 324.76. Its IUPAC name is 8-chloro-1-methyl-6-phenyl-4H-[1,2,4]triazolo[4,3-a][1,4]benzodiazepin-4-ol. It is a primary metabolite of alprazolam, formed by CYP3A4 oxidation. |
| Molecular Formula |
C17H13CLN4O
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|---|---|
| Molecular Weight |
324.76
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| Exact Mass |
324.078
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| CAS # |
30896-57-2
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| PubChem CID |
182017
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| Appearance |
Typically exists as solids at room temperature
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| Density |
1.5±0.1 g/cm3
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| Boiling Point |
559.2±60.0 °C at 760 mmHg
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| Melting Point |
160-162ºC
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| Flash Point |
292.0±32.9 °C
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| Vapour Pressure |
0.0±1.6 mmHg at 25°C
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| Index of Refraction |
1.731
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| LogP |
1.73
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
23
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| Complexity |
468
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CC1=NN=C2C(N=C(C3=CC=CC=C3)C4=C(C=CC(=C4)Cl)N12)O
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| InChi Key |
CEYGCFLPQFNPST-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C17H13ClN4O/c1-10-20-21-16-17(23)19-15(11-5-3-2-4-6-11)13-9-12(18)7-8-14(13)22(10)16/h2-9,17,23H,1H3
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| Chemical Name |
8-chloro-1-methyl-6-phenyl-4H-[1,2,4]triazolo[4,3-a][1,4]benzodiazepin-4-ol
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| Synonyms |
4-Hydroxyalprazolam
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.0792 mL | 15.3960 mL | 30.7920 mL | |
| 5 mM | 0.6158 mL | 3.0792 mL | 6.1584 mL | |
| 10 mM | 0.3079 mL | 1.5396 mL | 3.0792 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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