Size | Price | |
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500mg | ||
1g | ||
Other Sizes |
Targets |
mGluR5
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ln Vitro |
AZD 2066 (1-10 μM) hydrochloride inhibits Ca2+ responses with IC50 values of 27.2, 3.56, 96.2 and 380 nM in mGlu5/HEK cells and striatal, hippocampal and cortical cultures, respectively[4]. AZD 2066 (1-10 μM) hydrochloride inhibits DHPG bath-induced Ca2+ oscillatory responses and blocks the effects of DHPG or Quis in mGlu5/HEK cells[4].
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ln Vivo |
AZD 2066 (0.3-30 mg/kg, oral, 60 min) hydrochloride has a discriminative effect in rats[2]. AZD-2066 (10 µM, brain slice perfusion) hydrochloride alleviates dihydroxyphenylglycine (DHPG)-induced long-term depression (LTD) in mice treated with chronic social defeat stress (CSDS) via the BDNF/trkB signaling pathway[5]. AZD-2066 (5 mg/kg, intraperitoneal injection, 2 × 12 h) hydrochloride can alleviate the depressive behavior induced by chronic social defeat stress (CSDS) in mice[5].
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Animal Protocol |
Animal/Disease Models: Male Wistar rats (weighing 240-250 g)[2]
Doses: 0.03, 0.1, 0.3, 1, 3, 10, 30 mg/kg Route of Administration: Oral gavage (p.o.) Experimental Results: Caused full and dose-dependent AZD9272-appropriate responding. Animal/Disease Models: Depressive model (C57BL/6 mice were subjected to physical defeats that were generated by a CD-1 aggressor for 5 min every day for 10 days)[5] Doses: 5 mg/kg Route of Administration: Intraperitoneal injection (i.p.), 2 × 12 h Experimental Results: Increased social interaction ratio. Reversed time spent in interaction zone. Increased sucrose preference scores. |
References |
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Molecular Formula |
C19H17CL2N5O2
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Molecular Weight |
418.28
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CAS # |
934338-70-2
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Related CAS # |
AZD 2066;934282-55-0
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Appearance |
Typically exists as solids at room temperature
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SMILES |
ClC1=CC=CC(=C1)C1=CC([C@@H](C)OC2=NN=C(C3C=CN=CC=3)N2C)=NO1.Cl
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80: Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 :Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.3907 mL | 11.9537 mL | 23.9074 mL | |
5 mM | 0.4781 mL | 2.3907 mL | 4.7815 mL | |
10 mM | 0.2391 mL | 1.1954 mL | 2.3907 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.