| Size | Price | Stock | Qty |
|---|---|---|---|
| 10mg |
|
||
| 1g | |||
| Other Sizes |
| Animal Protocol |
Toxicity test (acute toxicity): Adult male Swiss white mice (approx. 25-28 g) were fasted for 20 hours before injection. Primin was diluted in Tween 80 (two drops) and saline (0.9%). A single intraperitoneal injection was given, and general effects were observed for 60 minutes and continued for about 72 hours after injection. The control group received solvent only. Doses tested for Primin were 14 mg/kg, 10 mg/kg, and 9 mg/kg. [2]
Antitumour activity test: Female mice (approx. 20-25 g) were divided into groups. Transplants of tumour fragments (about 1.5-2 mm) of sarcoma S-180 and Ehrlich carcinoma were made subcutaneously in the lateral thoracic wall of 6 mice. Starting 48 hours later, injections of test compounds were administered intraperitoneally once daily for 8 days. After treatment, tumours were removed and measured. The efficacy of treatment was estimated by the ellipsoid formula, and the volume of the control group was taken as 100%. [2] |
|---|---|
| Toxicity/Toxicokinetics |
In acute toxicity tests in mice, Primin caused deaths at the following doses: 14 mg/kg (3 out of 6 mice died), 10 mg/kg (2 out of 6 mice died), and 9 mg/kg (1 out of 6 mice died). The LD50 of Primin was determined to be 14 mg/kg. Observed behavioural effects included lethal hydric retention, labored breathing, and decreased muscle tone in some animals. [2]
|
| References | |
| Additional Infomation |
Primin is a 1,4-benzoquinone compound with a methoxy substituent at position 2 and an pentyl substituent at position 6. It possesses multiple functions, including hapten, metabolite, antibacterial agent, antifeedant, and allergen. Primin has been reported to be found in Cophinforma mamane, Primula halleri, and other organisms with relevant data. See also: Flower (part) of Primula veris.
Primin is known for its allergenic properties and as a strong sensitiser that induces contact dermatitis. The hydroquinone form of Primin, miconidin (2), was about three times less active than Primin in antitumour assays against sarcoma 180 and Ehrlich carcinoma. The quinone group is considered important for cytotoxic activity via formation of semiquinone radical anion. [2] |
| Molecular Formula |
C12H16O3
|
|---|---|
| Exact Mass |
208.109
|
| CAS # |
15121-94-5
|
| PubChem CID |
84800
|
| Appearance |
Typically exists as solid at room temperature
|
| Density |
1.1±0.1 g/cm3
|
| Boiling Point |
316.9±31.0 °C at 760 mmHg
|
| Melting Point |
77-79 °C
|
| Flash Point |
137.7±24.9 °C
|
| Vapour Pressure |
0.0±0.7 mmHg at 25°C
|
| Index of Refraction |
1.496
|
| LogP |
2.99
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
3
|
| Rotatable Bond Count |
5
|
| Heavy Atom Count |
15
|
| Complexity |
324
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
O(C([H])([H])[H])C1=C([H])C(C([H])=C(C1=O)C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H])=O
|
| InChi Key |
WLWIMKWZMGJRBS-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C12H16O3/c1-3-4-5-6-9-7-10(13)8-11(15-2)12(9)14/h7-8H,3-6H2,1-2H3
|
| Chemical Name |
2-methoxy-6-pentylcyclohexa-2,5-diene-1,4-dione
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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