| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg | |||
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| Targets |
Poly-D-lysine hydrobromide does not have a specific biological target as a drug; it is a cell culture reagent. Its positively charged surface interacts electrostatically with negatively charged cell membranes, promoting cell attachment and spreading on solid substrates. It is used to coat culture surfaces to enhance cell adhesion for a variety of cell types, including neurons, primary cells, and cell lines. The polymer is not intended to interact with specific receptors or enzymes.
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| ln Vitro |
Only low doses of poly-D-lysine injections cause immunogenicity in rabbits [1]. 50 nM, or about 0.8 μg/mL, is the estimated concentration of poly-D-lysine (PDL) needed to cut the amount of PrPres by 50% [2].
Poly-D-lysine hydrobromide is not evaluated for in vitro biological activity as a drug; it is a cell culture reagent. Its utility is assessed by its ability to promote cell adhesion, spreading, and proliferation on coated surfaces. Cells cultured on poly-D-lysine-coated surfaces show improved attachment and survival compared to uncoated surfaces. The compound does not have specific pharmacological activity. |
| ln Vivo |
Poly-D-lysine hydrobromide is not evaluated for in vivo biological activity as a drug; it is a cell culture reagent. It is not administered as a therapeutic agent. In research settings, it is used to coat implantable devices or scaffolds to promote cell adhesion and tissue integration. However, it is not intended for systemic administration.
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| Enzyme Assay |
There are no specific in vitro enzyme or receptor binding assays for Poly-D-lysine hydrobromide as a drug target. As a polymer, its properties are characterized by its molecular weight, polydispersity, and ability to promote cell adhesion. Cell adhesion assays are performed by coating culture plates with the polymer, seeding cells, and measuring cell attachment, spreading, and proliferation after 1-24 hours.
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| Cell Assay |
There are no specific in vitro cellular assays for Poly-D-lysine hydrobromide as a pharmacologically active compound. As a cell culture reagent, it is used to coat culture surfaces. Cells (e.g., neurons, fibroblasts, epithelial cells) are seeded onto poly-D-lysine-coated plates and cultured in appropriate media. Cell attachment is assessed by microscopy, and cell viability is assessed using MTT or Live/Dead assays. The polymer's effectiveness is evaluated by comparing cell behavior on coated vs. uncoated surfaces.
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| Animal Protocol |
There are no specific in vivo animal studies for Poly-D-lysine hydrobromide as a pharmacologically active compound. As a cell culture reagent, it is not administered to animals. In tissue engineering research, poly-D-lysine-coated scaffolds may be implanted in animal models to assess tissue integration and biocompatibility. However, specific protocols are not extensively reported.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of Poly-D-lysine hydrobromide are not applicable, as it is not a drug. As a high-molecular-weight polymer (MW 30,000-70,000), it is not absorbed systemically and does not distribute to tissues. The compound is used as a surface coating reagent and is not intended for administration.
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| Toxicity/Toxicokinetics |
The toxicity profile of Poly-D-lysine hydrobromide is generally considered low when used as a cell culture reagent. The compound is not intended for human therapeutic use. At high concentrations, polycationic polymers can be cytotoxic due to membrane disruption. Standard safety data indicate that the compound is relatively non-toxic at concentrations typically used for surface coating (1-100 μg/mL). No specific genotoxicity data are reported.
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| References | |
| Additional Infomation |
Poly-D-lysine hydrobromide (CAS 27964-99-4, PDL·HBr, MW 30,000-70,000) is a water-soluble, positively charged synthetic polymer used as a cell attachment factor in cell culture. It promotes cell adhesion to solid substrates through electrostatic interactions. The compound is supplied as a hydrobromide salt to aid solubility. It is available for research use only and is not intended for therapeutic applications.
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| Molecular Formula |
(C6H14N2O2)X.XHBR
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|---|---|
| Molecular Weight |
30000-70000
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| Exact Mass |
226.031
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| CAS # |
27964-99-4
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| Related CAS # |
Poly-D-lysine hydrobromide (MW 70000-150000);27964-99-4
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| PubChem CID |
87493163
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| Appearance |
White to yellow solid powder
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| Boiling Point |
343.7±0.0 °C at 760 mmHg
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| Flash Point |
161.7±0.0 °C
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| Vapour Pressure |
0.0±0.0 mmHg at 25°C
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| LogP |
0
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
11
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| Complexity |
106
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| Defined Atom Stereocenter Count |
1
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| SMILES |
C(CCN)C[C@H](C(=O)O)N.Br
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| InChi Key |
MEXAGTSTSPYCEP-NUBCRITNSA-N
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| InChi Code |
InChI=1S/C6H14N2O2.BrH/c7-4-2-1-3-5(8)6(9)10;/h5H,1-4,7-8H2,(H,9,10);1H/t5-;/m1./s1
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| Chemical Name |
(2R)-2,6-diaminohexanoic acid;hydrobromide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~50 mg/mL
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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